CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-022942
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Inositol hexakisphosphate kinase 2 
Protein Synonyms/Alias
 InsP6 kinase 2; P(i)-uptake stimulator; PiUS 
Gene Name
 IP6K2 
Gene Synonyms/Alias
 IHPK2; TCCCIA00113 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
15MDVEPRAKGVLLEPFubiquitination[1]
42FNETTLCKPLVPREHubiquitination[1]
61TLPAEMRKFTPQYKGubiquitination[1]
67RKFTPQYKGVVSVRFubiquitination[1, 2]
90CLIAYPLKGDHGIVDubiquitination[1]
129LETEKTPKDWVRQHRubiquitination[1]
145EEKMKSHKLEEEFEWubiquitination[1]
154EEEFEWLKKSEVLYYubiquitination[2]
155EEFEWLKKSEVLYYTubiquitination[1]
166LYYTVEKKGNISSQLubiquitination[1]
174GNISSQLKHYNPWSMubiquitination[1]
182HYNPWSMKCHQQQLQubiquitination[1]
202AKHRNQYKFILLENLubiquitination[2]
222VPCVLDLKMGTRQHGubiquitination[1, 3, 4]
236GDDASEEKAANQIRKubiquitination[1]
243KAANQIRKCQQSTSAubiquitination[1]
279MNKYHGRKLSVQGFKubiquitination[1, 5]
309ELLGPVLKKLTELKAubiquitination[1]
310LLGPVLKKLTELKAVubiquitination[1]
315LKKLTELKAVLERQEubiquitination[1]
370SAGAYAYKPIGASSVubiquitination[2]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [3] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [4] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [5] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302] 
Functional Description
 Converts inositol hexakisphosphate (InsP6) to diphosphoinositol pentakisphosphate (InsP7/PP-InsP5). Converts 1,3,4,5,6-pentakisphosphate (InsP5) to PP-InsP4. 
Sequence Annotation
 REGION 216 224 Substrate binding (By similarity).  
Keyword
 Alternative splicing; ATP-binding; Complete proteome; Kinase; Nucleotide-binding; Nucleus; Reference proteome; Transferase. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 426 AA 
Protein Sequence
MSPAFRAMDV EPRAKGVLLE PFVHQVGGHS CVLRFNETTL CKPLVPREHQ FYETLPAEMR 60
KFTPQYKGVV SVRFEEDEDR NLCLIAYPLK GDHGIVDIVD NSDCEPKSKL LRWTTNKKHH 120
VLETEKTPKD WVRQHRKEEK MKSHKLEEEF EWLKKSEVLY YTVEKKGNIS SQLKHYNPWS 180
MKCHQQQLQR MKENAKHRNQ YKFILLENLT SRYEVPCVLD LKMGTRQHGD DASEEKAANQ 240
IRKCQQSTSA VIGVRVCGMQ VYQAGSGQLM FMNKYHGRKL SVQGFKEALF QFFHNGRYLR 300
RELLGPVLKK LTELKAVLER QESYRFYSSS LLVIYDGKER PEVVLDSDAE DLEDLSEESA 360
DESAGAYAYK PIGASSVDVR MIDFAHTTCR LYGEDTVVHE GQDAGYIFGL QSLIDIVTEI 420
SEESGE 426 
Gene Ontology
 GO:0045111; C:intermediate filament cytoskeleton; IDA:HPA.
 GO:0005654; C:nucleoplasm; TAS:Reactome.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0052723; F:inositol hexakisphosphate 1-kinase activity; IEA:EC.
 GO:0052724; F:inositol hexakisphosphate 3-kinase activity; IEA:EC.
 GO:0000832; F:inositol hexakisphosphate 5-kinase activity; IEA:EC.
 GO:0008440; F:inositol-1,4,5-trisphosphate 3-kinase activity; IEA:InterPro.
 GO:0043647; P:inositol phosphate metabolic process; TAS:Reactome.
 GO:0030308; P:negative regulation of cell growth; IMP:UniProtKB.
 GO:0006817; P:phosphate ion transport; IEA:Compara.
 GO:0046854; P:phosphatidylinositol phosphorylation; IDA:UniProtKB.
 GO:0043065; P:positive regulation of apoptotic process; IMP:UniProtKB.
 GO:0060337; P:type I interferon-mediated signaling pathway; TAS:Reactome. 
Interpro
 IPR005522; IPK. 
Pfam
 PF03770; IPK 
SMART
  
PROSITE
  
PRINTS