CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-043428
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
  
Protein Name
 Microtubule-actin cross-linking factor 1, isoforms 1/2/3/5 
Protein Synonyms/Alias
  
Gene Name
 MACF1 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
587LKAEPLTKATHSSSTubiquitination[1]
844LQDSIKRKYSCDHNTubiquitination[1]
1677SVVEAIEKRIISETVubiquitination[1]
1734ESYLRTSKNLIDPNTubiquitination[1]
1744IDPNTAEKIGLLDLMubiquitination[1]
1763VHQESGFKLLPVKQLubiquitination[1]
1768GFKLLPVKQLAGGMVubiquitination[1]
1778AGGMVSLKSGRKVSIubiquitination[1]
1782VSLKSGRKVSIFRAVubiquitination[1]
1938TTEILSWKKAIESGIubiquitination[1]
1939TEILSWKKAIESGILubiquitination[1]
1991PCSKSHPKATASQSEubiquitination[1]
2023QRLLLLDKELMETLTubiquitination[1]
2041EYQTSPPKVVEIGHQubiquitination[1]
2051EIGHQRQKTPEGLQEubiquitination[1]
2086FQFSSQNKEYPDREDubiquitination[1]
2101CTTEKGKKTTVETEDubiquitination[1]
2123QDLFVEQKERNPNIDubiquitination[1]
2139LKVINKVKLEVQRQLubiquitination[1]
2264NVHPLDKKEMLKKTFubiquitination[1]
2274LKKTFLAKDDHKESQubiquitination[1]
2361VPSHTAVKLMEKLNMubiquitination[1]
2405HNVLMADKAISGVLDubiquitination[1]
2422TQTLCSVKDAVTVGLubiquitination[1]
2432VTVGLLDKETATRILubiquitination[1]
2452TGGIIDLKRGKKVSVubiquitination[1]
2537EAVRLLTKQVVDGGIubiquitination[1]
2571IGEDLAEKLKRVENLubiquitination[1]
2596KENISLPKAIKLDLIubiquitination[1]
2599ISLPKAIKLDLITSDubiquitination[1]
2608DLITSDLKREIQEVQubiquitination[1]
2636RLTLAEAKKEGLLTNubiquitination[1]
2637LTLAEAKKEGLLTNEubiquitination[1]
2671VPYSELVKKCKIDIEubiquitination[1]
2672PYSELVKKCKIDIESubiquitination[1]
2674SELVKKCKIDIESGQubiquitination[1]
2693VIPFSDIKDGVSDKVubiquitination[1]
2719VDFASTLKVLEAQANubiquitination[1]
2737IIDTATGKRLTLASAubiquitination[1]
2785TLVEAIEKRLISPELubiquitination[1]
2862ENQSAQEKVKVRVSDubiquitination[1]
2899PRMSSDAKEFISIINubiquitination[1]
2913NPHNLKGKSLGQVSLubiquitination[1]
3051DEHDSHIKSQPREMTubiquitination[1]
3104ALTLFSSKQANEGKVubiquitination[1]
3311LGSFLPEKLFKGVSQubiquitination[1]
3319LFKGVSQKENTGQQNubiquitination[1]
3422RNIEMRTKQIQPLELubiquitination[1]
3490KDAKNLQKSLSSVSDubiquitination[1]
3538LRAWVGNKNLILNSKubiquitination[1]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961
Functional Description
  
Sequence Annotation
  
Keyword
 Actin-binding; Calcium; Complete proteome; Reference proteome; Repeat. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 7592 AA 
Protein Sequence
MSSSDEETLS ERSCRSERSC RSERSYRSER SGSLSPCPPG DTLPWNLPLH EQKKRKSQDS 60
VLDPAERAVV RVADERDRVQ KKTFTKWVNK HLMKVRKHIN DLYEDLRDGH NLISLLEVLS 120
GIKLEPAGLK TLRLVSMPSW CHTNNEEQAE EDDDDVPREK GRMRFHRLQN VQIALDFLKQ 180
RQVKLVNIRN DDITDGNPKL TLGLIWTIIL HFQISDIYIS GESGDMSAKE KLLLWTQKVT 240
AGYTGIKCTN FSSCWSDGKM FNALIHRYRP DLVDMERVQI QSNRENLEQA FEVAERLGVT 300
RLLDAEDVDV PSPDEKSVIT YVSSIYDAFP KVPEGGEGIS ATEVDSRWQE YQSRVDSLIP 360
WIKQHTILMS DKTFPQNPVE LKALYNQYIH FKETEILAKE REKGRIEELY KLLEVWIEFG 420
RIKLPQGYHP NDVEEEWGKL IIEMLEREKS LRPAVERLEL LLQIANKIQN GALNCEEKLT 480
LAKNTLQADA AHLESGQPVQ CESDVIMYIQ ECEGLIRQLQ VDLQILRDEN YYQLEELAFR 540
VMRLQDELVT LRLECTNLYR KGHFTSLELV PPSTLTTTHL KAEPLTKATH SSSTSWFRKP 600
MTRAELVAIS SSEDEGNLRF VYELLSWVEE MQMKLERAEW GNDLPSVELQ LETQQHIHTS 660
VEELGSSVKE ARLYEGKMSQ NFHTSYAETL GKLETQYCKL KETSSFRMRH LQSLHKFVSR 720
ATAELIWLNE KEEEELAYDW SDNNSNISAK RNYFSELTME LEEKQDVFRS LQDTAELLSL 780
ENHPAKQTVE AYSAAVQSQL QWMKQLCLCV EQHVKENTAY FQFFSDAREL ESFLRNLQDS 840
IKRKYSCDHN TSLSRLEDLL QDSMDEKEQL IQSKSSVASL VGRSKTIVQL KPRSPDHVLK 900
NTISVKAVCD YRQIEITICK NDECVLEDNS QRTKWKVISP TGNEAMVPSV CFLIPPPNKD 960
AIEMASRVEQ SYQKVMALWH QLHVNTKSLI SWNYLRKDLD LVQTWNLEKL RSSAPGECHQ 1020
IMKNLQAHYE DFLQDSRDSV LFSVADRLRL EEEVEACKAR FQHLMKSMEN EDKEETVAKM 1080
YISELKNIRL RLEEYEQRVV KRIQSLASSR TDRDAWQDNA LRIAEQEHTQ EDLQQLRSDL 1140
DAVSMKCDSF LHQSPSSSSV PTLRSELNLL VEKMDHVYGL STVYLNKLKT VDVIVRSIQD 1200
AELLVKGYEI KLSQEEVVLA DLSALEAHWS TLRHWLSDVK DKNSVFSVLD EEIAKAKVVA 1260
EQMSRLTPER NLDLERYQEK GSQLQERWHR VIAQLEIRQS ELESIQEVLG DYRACHGTLI 1320
KWIEETTAQQ EMMKPGQAED SRVLSEQLSQ QTALFAEIER NQTKLDQCQK FSQQYSTIVK 1380
DYELQLMTYK AFVESQQKSP GKRRRMLSSS DAITQEFMDL RTRYTALVTL TTQHVKYISD 1440
ALRRLEEEEK VVEEEKQEHV EKVKELLGWV STLARNTQGK ATSSETKEST DIEKAILEQQ 1500
VLSEELTTKK EQVSEAIKTS QIFLAKHGHK LSEKEKKQIS EQLNALNKAY HDLCDGSANQ 1560
LQQLQSQLAH QTEQKECRAV AGVIDLGTVE IFPIFKAMQK GLLDQDTGLV LLESQVIMSG 1620
LIAPETGENL SLEEGIARNL INPQMYQQLR ELQDALALIS RLTESRGPLS VVEAIEKRII 1680
SETVGLKILE AHLATGGFSL SPSENCINLE EAFHQGLISA WLHSVLESYL RTSKNLIDPN 1740
TAEKIGLLDL MQRCIVHQES GFKLLPVKQL AGGMVSLKSG RKVSIFRAVQ EGLIDRQVTV 1800
RLLEAQLFAG GIVDPRTGHR LTVEEAVRHN LIDQDMACAI LIRQLQTGGI IDTVTGQRLT 1860
IDEAVSNDLV AAKIALVILE SLWSFMGLLW PESGEILPIT DALEQGIVST ELAHKILSNR 1920
QHIKALFLPA TTEILSWKKA IESGILDRDL ANNLKSICIP DVMPHMQLAD SAEQNINPGA 1980
AVLPCSKSHP KATASQSENL LFQLMTHSYI NVQNGQRLLL LDKELMETLT SRDEYQTSPP 2040
KVVEIGHQRQ KTPEGLQESA NVKISGTFSS GWTVRLPEFQ FSSQNKEYPD REDCTTEKGK 2100
KTTVETEDSS VENPEQDLFV EQKERNPNID ALKVINKVKL EVQRQLIGTQ REDQTAVSVR 2160
ENASRGHLLT IPPAEAEGVP LVVDKDVFSV ETPKKEHQPL RNTSFTCQNE QAHTLETEYI 2220
HDETGGSHIK PQSKKLQVQV KKTLGIKLEL KSETDGNVHP LDKKEMLKKT FLAKDDHKES 2280
QEAQNIAGGS MMMSEKTDEE DSGREIFLSC SHPLELLEEA TLNVLSAQLL DGGIFHEQTG 2340
QKLLLNEAIS RGIVPSHTAV KLMEKLNMFQ GFFDSQTCES LTTEEVINEG LMDEKLLHNV 2400
LMADKAISGV LDPRTQTLCS VKDAVTVGLL DKETATRILE RQVVTGGIID LKRGKKVSVT 2460
LASTLGLVDV ADQPELINLE KASKGRDAEK TVRERLISLQ METTGLIDPD SKAPLTVVQS 2520
IDRGLLEREE AVRLLTKQVV DGGIIHHISG MRLSVDNAFR HGLIGEDLAE KLKRVENLNI 2580
HQIFNPETKE NISLPKAIKL DLITSDLKRE IQEVQAFTGN FVDLISGQRL TLAEAKKEGL 2640
LTNEAVLSPG MMHGIVDPEN CRIVPYSELV KKCKIDIESG QRYLEVIPFS DIKDGVSDKV 2700
LTLSQAIQLG KVDFASTLKV LEAQANTGGI IDTATGKRLT LASALEEKLV DENMVRIIAS 2760
HQVLNGGIVD IFSDQRVTLV EAIEKRLISP ELANMIQIDS SEFSDHRAQI EKQEGIEVCA 2820
LQNEFLGKDM LIACNQTAEM SCNKVEESER LFQVENQSAQ EKVKVRVSDG EQAKKSREIS 2880
LKEFGCKDQR KPRMSSDAKE FISIINPHNL KGKSLGQVSL THPYSECDFK LKEVARNNMG 2940
NDTNEEQEKA VTKIEIISHM KQSTSCLDSE EIRENQGEVI LEVQETYCET SGKLPSEQVL 3000
QQPMNARVKS KREKREVIVE ESIRTCKPAF LSEEKLYQET AIRDEHDSHI KSQPREMTSS 3060
EKGKEADTEM GFSITFKIEE SSSQVVPQGI SVKHLDALTL FSSKQANEGK VNNLSLCLTL 3120
KPEENLSREI ACGAQSEPFP CMTPRPEGLH YQESDGKAQV TGPSQISKTD KSFQGTTRQE 3180
TNYQDSWVTS KTKETKHQIS SSNECKEKSY QEVSFDPARG LKLEEITVSR PDSKEVRYLE 3240
FSDRKDLHHQ GSKSDDKLCG TLKSEIATQE LTGEKFLEMA NPNVAGLEAG SIEDIVTQRG 3300
SRVLGSFLPE KLFKGVSQKE NTGQQNAIIS PTVLETSEEK TVSLTVCSAV KTEKTPQEKL 3360
RESPGSEQTP FMTAPEGKGN GGVNPEPFRA TQNVFTRQLC LEHDEKLVSY LSLLRNIEMR 3420
TKQIQPLELN LAELQDLLCQ AKVLERELKD LTTLVSQELE CVNQIIISQP QEVPAQLLKA 3480
LEKDAKNLQK SLSSVSDTWN SRLLHFQNAV EIEKTKVLNQ HTQLEGRLQD LRAWVGNKNL 3540
ILNSKGSNSE IDVDSLNLCL QQYEDLKQPM AERKAQLDAL AFDIQFFISE HAQDLSPQQN 3600
RQMLRLLNEL QRSFQDILEQ TAAQVDALQG HLQQMEQEAL VKTLQKQQNT CHQQLEDLCS 3660
WVGQAERALA GHQGRTTQQD LSALQKNQSD LKDLQDDIQN RATSFATVVK DIEGFMEENQ 3720
TKLSPRELTA LREKLHQAKE QYEALQEETR VAQKELEEAV TSALQQETEK SKAAKELAEN 3780
KKKIDALLDW VTSVGSSGGQ LLTNLPGMEQ LSGASLEKGA LDTTDGYMGV NQAPEKLDKQ 3840
CEMMKARHQE LLSQQQNFIL ATQSAQAFLD QHGHNLTPEE QQMLQQKLGE LKEQYSTSLA 3900
QSEAELKQVQ TLQDELQKFL QDHKEFESWL ERSEKELENM HKGGSSPETL PSLLKRQGSF 3960
SEDVISHKGD LRFVTISGQK VLDMENSFKE GKEPSEIGNL VKDKLKDATE RYTALHSKCT 4020
RLGSHLNMLL GQYHQFQNSA DSLQAWMQAC EANVEKLLSD TVASDPGVLQ EQLATTKQLQ 4080
EELAEHQVPV EKLQKVARDI MEIEGEPAPD HRHVQETTDS ILSHFQSLSY SLAERSSLLQ 4140
KAIAQSQSVQ ESLESLLQSI GEVEQNLEGK QVSSLSSGVI QEALATNMKL KQDIARQKSS 4200
LEATREMVTR FMETADSTTA AVLQGKLAEV SQRFEQLCLQ QQEKESSLKK LLPQAEMFEH 4260
LSGKLQQFME NKSRMLASGN QPDQDITHFF QQIQELNLEM EDQQENLDTL EHLVTELSSC 4320
GFALDLCQHQ DRVQNLRKDF TELQKTVKER EKDASSCQEQ LDEFRKLVRT FQKWLKETEG 4380
SIPPTETSMS AKELEKQIEH LKSLLDDWAS KGTLVEEINC KGTSLENLIM EITAPDSQGK 4440
TGSILPSVGS SVGSVNGYHT CKDLTEIQCD MSDVNLKYEK LGGVLHERQE SLQAILNRME 4500
EVHKEANSVL QWLESKEEVL KSMDAMSSPT KTETVKAQAE SNKAFLAELE QNSPKIQKVK 4560
EALAGLLVTY PNSQEAENWK KIQEELNSRW ERATEVTVAR QRQLEESASH LACFQAAESQ 4620
LRPWLMEKEL MMGVLGPLSI DPNMLNAQKQ QVQFMLKEFE ARRQQHEQLN EAAQGILTGP 4680
GDVSLSTSQV QKELQSINQK WVELTDKLNS RSSQIDQAIV KSTQYQELLQ DLSEKVRAVG 4740
QRLSVQSAIS TQPEAVKQQL EETSEIRSDL EQLDHEVKEA QTLCDELSVL IGEQYLKDEL 4800
KKRLETVALP LQGLEDLAAD RINRLQAALA STQQFQQMFD ELRTWLDDKQ SQQAKNCPIS 4860
AKLERLQSQL QENEEFQKSL NQHSGSYEVI VAEGESLLLS VPPGEEKRTL QNQLVELKNH 4920
WEELSKKTAD RQSRLKDCMQ KAQKYQWHVE DLVPWIEDCK AKMSELRVTL DPVQLESSLL 4980
RSKAMLNEVE KRRSLLEILN SAADILINSS EADEDGIRDE KAGINQNMDA VTEELQAKTG 5040
SLEEMTQRLR EFQESFKNIE KKVEGAKHQL EIFDALGSQA CSNKNLEKLR AQQEVLQALE 5100
PQVDYLRNFT QGLVEDAPDG SDASQLLHQA EVAQQEFLEV KQRVNSGCVM MENKLEGIGQ 5160
FHCRVREMFS QLADLDDELD GMGAIGRDTD SLQSQIEDVR LFLNKIHVLK LDIEASEAEC 5220
RHMLEEEGTL DLLGLKRELE ALNKQCGKLT ERGKARQEQL ELTLGRVEDF YRKLKGLNDA 5280
TTAAEEAEAL QWVVGTEVEI INQQLADFKM FQKEQVDPLQ MKLQQVNGLG QGLIQSAGKD 5340
CDVQGLEHDM EEINARWNTL NKKVAQRIAQ LQEALLHCGK FQDALEPLLS WLADTEELIA 5400
NQKPPSAEYK VVKAQIQEQK LLQRLLDDRK ATVDMLQAEG GRIAQSAELA DREKITGQLE 5460
SLESRWTELL SKAAARQKQL EDILVLAKQF HETAEPISDF LSVTEKKLAN SEPVGTQTAK 5520
IQQQIIRHKA LEEDIENHAT DVHQAVKIGQ SLSSLTSPAE QGVLSEKIDS LQARYSEIQD 5580
RCCRKAALLD QALSNARLFG EDEVEVLNWL AEVEDKLSSV FVKDFKQDVL HRQHADHLAL 5640
NEEIVNRKKN VDQAIKNGQA LLKQTTGEEV LLIQEKLDGI KTRYADITVT SSKALRTLEQ 5700
ARQLATKFQS TYEELTGWLR EVEEELATSG GQSPTGEQIP QFQQRQKELK KEVMEHRLVL 5760
DTVNEVSRAL LELVPWRARE GLDKLVSDAN EQYKLVSDTI GQRVDEIDAA IQRSQQYEQA 5820
ADAELAWVAE TKRKLMALGP IRLEQDQTTA QLQVQKAFSI DIIRHKDSMD ELFSHRSEIF 5880
GTCGEEQKTV LQEKTESLIQ QYEAISLLNS ERYARLERAQ VLVNQFWETY EELSPWIEET 5940
RALIAQLPSP AIDHEQLRQQ QEEMRQLRES IAEHKPHIDK LLKIGPQLKE LNPEEGEMVE 6000
EKYQKAENMY AQIKEEVRQR ALALDEAVSQ STQFHDKIEP MLETLENLSS RLRMPPLIPA 6060
EVDKIRECIS DNKSATVELE KLQPSFEALK RRGEELIGRS QGADKDLAAK EIQDKLDQMV 6120
FFWEDIKARA EEREIKFLDV LELAEKFWYD MAALLTTIKD TQDIVHDLES PGIDPSIIKQ 6180
QVEAAETIKE ETDGLHEELE FIRILGADLI FACGETEKPE VRKSIDEMNN AWENLNKTWK 6240
ERLEKLEDAM QAAVQYQDTL QAMFDWLDNT VIKLCTMPPV GTDLNTVKDQ LNEMKEFKVE 6300
VYQQQIEMEK LNHQGELMLK KATDETDRDI IREPLTELKH LWENLGEKIA HRQHKLEGAL 6360
LALGQFQHAL EELMSWLTHT EELLDAQRPI SGDPKVIEVE LAKHHVLKND VLAHQATVET 6420
VNKAGNELLE SSAGDDASSL RSRLEAMNQC WESVLQKTEE REQQLQSTLQ QAQGFHSEIE 6480
DFLLELTRME SQLSASKPTG GLPETAREQL DTHMELYSQL KAKEETYNQL LDKGRLMLLS 6540
RDDSGSGSKT EQSVALLEQK WHVVSSKMEE RKSKLEEALN LATEFQNSLQ EFINWLTLAE 6600
QSLNIASPPS LILNTVLSQI EEHKVFANEV NAHRDQIIEL DQTGNQLKFL SQKQDVVLIK 6660
NLLVSVQSRW EKVVQRSIER GRSLDDARKR AKQFHEAWKK LIDWLEDAES HLDSELEISN 6720
DPDKIKLQLS KHKEFQKTLG GKQPVYDTTI RTGRALKEKT LLPEDSQKLD NFLGEVRDKW 6780
DTVCGKSVER QHKLEEALLF SGQFMDALQA LVDWLYKVEP QLAEDQPVHG DLDLVMNLMD 6840
AHKVFQKELG KRTGTVQVLK RSGRELIENS RDDTTWVKGQ LQELSTRWDT VCKLSVSKQS 6900
RLEQALKQAE VFRDTVHMLL EWLSEAEQTL RFRGALPDDT EALQSLIDTH KEFMKKVEEK 6960
RVDVNSAVAM GEVILAVCHP DCITTIKHWI TIIRARFEEV LTWAKQHQQR LETALSELVA 7020
NAELLEELLA WIQWAETTLI QRDQEPIPQN IDRVKALIAE HQTFMEEMTR KQPDVDRVTK 7080
TYKRKNIEPT HAPFIEKSRS GGRKSLSQPT PPPMPILSQS EAKNPRINQL SARWQQVWLL 7140
ALERQRKLND ALDRLEELKE FANFDFDVWR KKYMRWMNHK KSRVMDFFRR IDKDQDGKIT 7200
RQEFIDGILA SKFPTTKLEM TAVADIFDRD GDGYIDYYEF VAALHPNKDA YRPTTDADKI 7260
EDEVTRQVAQ CKCAKRFQVE QIGENKYRFF LGNQFGDSQQ LRLVRILRST VMVRVGGGWM 7320
ALDEFLVKND PCRVHHPGSK IKRSDSSSSI SSQSPIARGR TNIELREKFI LPEGASQGMT 7380
PFRSRGRRSK PSSRAASPTR SSSSASQSNH SCTSMPSSPA TPASGTKTSL QFSRCYDKPW 7440
LVNSKAGTPI RDSHSPDLQL PTPEVIPSSG SKLKRPTPTF HSSRTSLAGD TSNSSSPAST 7500
GAKTNRADPK KSASRPGSRA GSRAGSRASS RRGSDASDFD LLETQSACSD TSESSAAGGQ 7560
GNSRRGLNKP SKIPTMSKKT TTASPRTPGP KR 7592 
Gene Ontology
 GO:0005856; C:cytoskeleton; IEA:InterPro.
 GO:0005509; F:calcium ion binding; IEA:InterPro.
 GO:0007050; P:cell cycle arrest; IEA:InterPro. 
Interpro
 IPR001589; Actinin_actin-bd_CS.
 IPR001715; CH-domain.
 IPR011992; EF-hand-like_dom.
 IPR018247; EF_Hand_1_Ca_BS.
 IPR002048; EF_hand_dom.
 IPR003108; GAS2_dom.
 IPR001101; Plectin_repeat.
 IPR018159; Spectrin/alpha-actinin.
 IPR002017; Spectrin_repeat. 
Pfam
 PF00307; CH
 PF13499; EF_hand_5
 PF02187; GAS2
 PF00681; Plectin
 PF00435; Spectrin 
SMART
 SM00033; CH
 SM00054; EFh
 SM00243; GAS2
 SM00250; PLEC
 SM00150; SPEC 
PROSITE
 PS00019; ACTININ_1
 PS00020; ACTININ_2
 PS50021; CH
 PS00018; EF_HAND_1
 PS50222; EF_HAND_2
 PS51460; GAR 
PRINTS