CPLM-020929

Genbank Nucleotide ID

Cytochrome b-c1 complex subunit 2, mitochondrial

Protein Synonyms/Alias

Complex III subunit 2; Core protein II; Ubiquinol-cytochrome-c reductase complex core protein 2

Mus musculus (Mouse)

Position	Peptide	Type	References
42	PQDLEFTKLPNGLVI	acetylation	[1]
66	SRIGLFVKAGSRYED	acetylation	[1, 2, 3, 4, 5]
66	SRIGLFVKAGSRYED	succinylation	[5]
92	LASSLTTKGASSFKI	acetylation	[2, 5]
92	LASSLTTKGASSFKI	succinylation	[5]
92	LASSLTTKGASSFKI	ubiquitination	[6]
98	TKGASSFKITRGIEA	acetylation	[1, 2, 3, 4]
159	AALRSQLKIDKAVAF	acetylation	[1, 2, 3, 4, 7]
199	CPDYRMGKITSEELH	acetylation	[1, 4, 5]
199	CPDYRMGKITSEELH	succinylation	[5]
231	GVSHSVLKQVAEQFL	acetylation	[2]
250	GLGLAGAKAKYRGGE	acetylation	[2, 4, 5, 7]
250	GLGLAGAKAKYRGGE	succinylation	[5]
315	LLSQSVAKGSHQPFD	acetylation	[1]
375	SADVQAAKNKLKAGY	ubiquitination	[6]
435	AKKFVSGKKSMAASG	acetylation	[1, 3]

[1] Quantification of mitochondrial acetylation dynamics highlights prominent sites of metabolic regulation.
Still AJ, Floyd BJ, Hebert AS, Bingman CA, Carson JJ, Gunderson DR, Dolan BK, Grimsrud PA, Dittenhafer-Reed KE, Stapleton DS, Keller MP, Westphall MS, Denu JM, Attie AD, Coon JJ, Pagliarini DJ.
J Biol Chem. 2013 Jul 17;. [PMID: 23864654]
[2] Quantitative assessment of the impact of the gut microbiota on lysine epsilon-acetylation of host proteins using gnotobiotic mice.
Simon GM, Cheng J, Gordon JI.
Proc Natl Acad Sci U S A. 2012 Jul 10;109(28):11133-8. [PMID: 22733758]
[3] Calorie restriction and SIRT3 trigger global reprogramming of the mitochondrial protein acetylome.
Hebert AS, Dittenhafer-Reed KE, Yu W, Bailey DJ, Selen ES, Boersma MD, Carson JJ, Tonelli M, Balloon AJ, Higbee AJ, Westphall MS, Pagliarini DJ, Prolla TA, Assadi-Porter F, Roy S, Denu JM, Coon JJ.
Mol Cell. 2013 Jan 10;49(1):186-99. [PMID: 23201123]
[4] Label-free quantitative proteomics of the lysine acetylome in mitochondria identifies substrates of SIRT3 in metabolic pathways.
Rardin MJ, Newman JC, Held JM, Cusack MP, Sorensen DJ, Li B, Schilling B, Mooney SD, Kahn CR, Verdin E, Gibson BW.
Proc Natl Acad Sci U S A. 2013 Apr 16;110(16):6601-6. [PMID: 23576753]
[5] SIRT5-Mediated Lysine Desuccinylation Impacts Diverse Metabolic Pathways.
Park J, Chen Y, Tishkoff DX, Peng C, Tan M, Dai L, Xie Z, Zhang Y, Zwaans BM, Skinner ME, Lombard DB, Zhao Y.
Mol Cell. 2013 Jun 27;50(6):919-30. [PMID: 23806337]
[6] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023]
[7] Substrate and functional diversity of lysine acetylation revealed by a proteomics survey.
Kim SC, Sprung R, Chen Y, Xu Y, Ball H, Pei J, Cheng T, Kho Y, Xiao H, Xiao L, Grishin NV, White M, Yang XJ, Zhao Y.
Mol Cell. 2006 Aug;23(4):607-18. [PMID: 16916647]

Functional Description

This is a component of the ubiquinol-cytochrome c reductase complex (complex III or cytochrome b-c1 complex), which is part of the mitochondrial respiratory chain. The core protein 2 is required for the assembly of the complex (By similarity).

MOD_RES 159 159 N6-acetyllysine.
MOD_RES 250 250 N6-acetyllysine.

Acetylation; Complete proteome; Direct protein sequencing; Electron transport; Membrane; Mitochondrion; Mitochondrion inner membrane; Reference proteome; Respiratory chain; Transit peptide; Transport.

UniProt (SWISSPROT/TrEMBL); GenBank; EMBL

GO:0005743; C:mitochondrial inner membrane; IDA:MGI.
GO:0005750; C:mitochondrial respiratory chain complex III; IEA:Compara.
GO:0046872; F:metal ion binding; IEA:InterPro.
GO:0004222; F:metalloendopeptidase activity; IEA:InterPro.
GO:0022900; P:electron transport chain; IEA:UniProtKB-KW.
GO:0006508; P:proteolysis; IEA:InterPro.

IPR011249; Metalloenz_LuxS/M16.
IPR011237; Pept_M16_dom.
IPR011765; Pept_M16_N.
IPR001431; Pept_M16_Zn_BS.
IPR007863; Peptidase_M16_C.

PF00675; Peptidase_M16
PF05193; Peptidase_M16_C