UniProt Accession

 GNAI2_RAT; P04897 

Genbank Protein ID

 M12672; M17528; BC078806 

Genbank Nucleotide ID

 AAA41260.1; AAA40824.1; AAH78806.1 

Protein Name

 Guanine nucleotide-binding protein G(i) subunit alpha-2 

Protein Synonyms/Alias

 Adenylate cyclase-inhibiting G alpha protein 

Gene Name

 Gnai2 

Gene Synonyms/Alias

 Gnai-2 

Created Date

 July 27, 2013 

Organism

 Rattus norvegicus (Rat) 

NCBI Taxa ID

 10116 

Lysine Modification

Position	Peptide	Type	References
35	EKAAREVKLLLLGAG	acetylation	[1]
35	EKAAREVKLLLLGAG	ubiquitination	[2]
307	AASYIQSKFEDLNKR	acetylation	[1]

Reference

 [1] Proteomic analysis of lysine acetylation sites in rat tissues reveals organ specificity and subcellular patterns.
 Lundby A, Lage K, Weinert BT, Bekker-Jensen DB, Secher A, Skovgaard T, Kelstrup CD, Dmytriyev A, Choudhary C, Lundby C, Olsen JV.
 Cell Rep. 2012 Aug 30;2(2):419-31. [PMID: 22902405]
 [2] Synaptic protein ubiquitination in rat brain revealed by antibody-based ubiquitome analysis.
 Na CH, Jones DR, Yang Y, Wang X, Xu Y, Peng J.
 J Proteome Res. 2012 Sep 7;11(9):4722-32. [PMID: 22871113] 

Functional Description

 Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems. The G(i) proteins are involved in hormonal regulation of adenylate cyclase: they inhibit the cyclase in response to beta-adrenergic stimuli. May play a role in cell division. 

Sequence Annotation

 NP_BIND 40 47 GTP (By similarity).
 NP_BIND 176 182 GTP (By similarity).
 NP_BIND 201 205 GTP (By similarity).
 NP_BIND 270 273 GTP (By similarity).
 METAL 47 47 Magnesium (By similarity).
 METAL 182 182 Magnesium (By similarity).
 BINDING 327 327 GTP; via amide nitrogen (By similarity).
 LIPID 2 2 N-myristoyl glycine (By similarity).
 LIPID 3 3 S-palmitoyl cysteine (By similarity).  

Keyword

 Cell cycle; Cell division; Cell membrane; Complete proteome; Cytoplasm; Cytoskeleton; Direct protein sequencing; GTP-binding; Lipoprotein; Magnesium; Membrane; Metal-binding; Myristate; Nucleotide-binding; Palmitate; Polymorphism; Reference proteome; Transducer. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 355 AA 

Protein Sequence

Gene Ontology

 GO:0005813; C:centrosome; ISS:UniProtKB.
 GO:0005829; C:cytosol; IDA:RGD.
 GO:0005834; C:heterotrimeric G-protein complex; IBA:RefGenome.
 GO:0045121; C:membrane raft; IDA:RGD.
 GO:0030496; C:midbody; ISS:UniProtKB.
 GO:0005634; C:nucleus; IDA:RGD.
 GO:0031683; F:G-protein beta/gamma-subunit complex binding; IBA:RefGenome.
 GO:0001664; F:G-protein coupled receptor binding; IBA:RefGenome.
 GO:0005525; F:GTP binding; IEA:UniProtKB-KW.
 GO:0003924; F:GTPase activity; IBA:RefGenome.
 GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
 GO:0004871; F:signal transducer activity; IBA:RefGenome.
 GO:0000186; P:activation of MAPKK activity; IMP:RGD.
 GO:0001973; P:adenosine receptor signaling pathway; IDA:RGD.
 GO:0007193; P:adenylate cyclase-inhibiting G-protein coupled receptor signaling pathway; IBA:RefGenome.
 GO:0007049; P:cell cycle; IEA:UniProtKB-KW.
 GO:0051301; P:cell division; ISS:UniProtKB.
 GO:0008283; P:cell proliferation; IEA:Compara.
 GO:0007213; P:G-protein coupled acetylcholine receptor signaling pathway; IEA:Compara.
 GO:0007214; P:gamma-aminobutyric acid signaling pathway; IDA:RGD.
 GO:0007243; P:intracellular protein kinase cascade; IMP:RGD.
 GO:0050805; P:negative regulation of synaptic transmission; IDA:RGD.
 GO:0008284; P:positive regulation of cell proliferation; IMP:RGD.
 GO:0051924; P:regulation of calcium ion transport; IDA:RGD. 

Interpro

 IPR001408; Gprotein_alpha_I.
 IPR001019; Gprotein_alpha_su.
 IPR011025; GproteinA_insert.
 IPR027417; P-loop_NTPase. 

Pfam

 PF00503; G-alpha 

SMART

 SM00275; G_alpha 

PROSITE

  

PRINTS

 PR00318; GPROTEINA.
 PR00441; GPROTEINAI.