UniProt Accession

 G3P1_YEAST; P00360; D6VWD1 

Genbank Protein ID

 V01302; Z49327; AY693001; BK006943 

Genbank Nucleotide ID

 CAA24609.1; CAA89343.1; AAT93020.1; DAA08747.1 

Protein Name

 Glyceraldehyde-3-phosphate dehydrogenase 1 

Protein Synonyms/Alias

 GAPDH 1 

Gene Name

 TDH1 

Gene Synonyms/Alias

 GPD1; SSS2; YJL052W; J1154 

Created Date

 July 27, 2013 

Organism

 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 

NCBI Taxa ID

 559292 

Lysine Modification

Position	Peptide	Type	References
63	GTVSHDDKHIIIDGV	acetylation	[1]
71	HIIIDGVKIATYQER	acetylation	[1]
101	VDSTGVFKELDTAQK	acetylation	[1]
137	VVGVNHTKYTPDKKI	acetylation	[1]
184	HSMTATQKTVDGPSH	acetylation	[2]
249	DLTVKLEKEATYDQI	acetylation	[1]
257	EATYDQIKKAVKAAA	acetylation	[1]
331	DLIEYVAKA******	acetylation	[1]

Reference

 [1] Proteome-wide analysis of lysine acetylation suggests its broad regulatory scope in Saccharomyces cerevisiae.
 Henriksen P, Wagner SA, Weinert BT, Sharma S, Bacinskaja G, Rehman M, Juffer AH, Walther TC, Lisby M, Choudhary C.
 Mol Cell Proteomics. 2012 Nov;11(11):1510-22. [PMID: 22865919]
 [2] Role of lysine-183 in D-glyceraldehyde-3-phosphate dehydrogenases. Properties of the N-acetylated yeast, sturgeon muscle and rabbit muscle enzymes.
 Foucault G, Nakano M, Pudles J.
 Eur J Biochem. 1978 Feb 1;83(1):113-23. [PMID: 342241] 

Functional Description

  

Sequence Annotation

 NP_BIND 11 12 NAD (By similarity).
 REGION 149 151 Glyceraldehyde 3-phosphate binding (By
 REGION 209 210 Glyceraldehyde 3-phosphate binding (By
 ACT_SITE 150 150 Nucleophile (By similarity).
 BINDING 33 33 NAD (By similarity).
 BINDING 78 78 NAD; via carbonyl oxygen (By similarity).
 BINDING 180 180 Glyceraldehyde 3-phosphate (By
 BINDING 232 232 Glyceraldehyde 3-phosphate (By
 BINDING 314 314 NAD (By similarity).  

Keyword

 Complete proteome; Cytoplasm; Direct protein sequencing; Glycolysis; NAD; Oxidoreductase; Reference proteome. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 332 AA 

Protein Sequence

Gene Ontology

 GO:0009277; C:fungal-type cell wall; IDA:SGD.
 GO:0005811; C:lipid particle; IDA:SGD.
 GO:0005739; C:mitochondrion; IDA:SGD.
 GO:0005886; C:plasma membrane; IDA:SGD.
 GO:0004365; F:glyceraldehyde-3-phosphate dehydrogenase (NAD+) (phosphorylating) activity; IDA:SGD.
 GO:0051287; F:NAD binding; IEA:InterPro.
 GO:0050661; F:NADP binding; IEA:InterPro.
 GO:0006094; P:gluconeogenesis; IEP:SGD.
 GO:0006096; P:glycolysis; IEP:SGD. 

Interpro

 IPR020831; GlycerAld/Erythrose_P_DH.
 IPR020830; GlycerAld_3-P_DH_AS.
 IPR020829; GlycerAld_3-P_DH_cat.
 IPR020828; GlycerAld_3-P_DH_NAD(P)-bd.
 IPR006424; Glyceraldehyde-3-P_DH_1.
 IPR016040; NAD(P)-bd_dom. 

Pfam

 PF02800; Gp_dh_C
 PF00044; Gp_dh_N 

SMART

 SM00846; Gp_dh_N 

PROSITE

 PS00071; GAPDH 

PRINTS

 PR00078; G3PDHDRGNASE.