UniProt Accession

 IF4E_MOUSE; P63073; P20415 

Genbank Protein ID

 M61731; BC010759; BC085087 

Genbank Nucleotide ID

 AAA37545.1; AAH10759.1; AAH85087.1 

Protein Name

 Eukaryotic translation initiation factor 4E 

Protein Synonyms/Alias

 eIF-4E; eIF4E; mRNA cap-binding protein; eIF-4F 25 kDa subunit 

Gene Name

 Eif4e 

Gene Synonyms/Alias

  

Created Date

 July 27, 2013 

Organism

 Mus musculus (Mouse) 

NCBI Taxa ID

 10090 

Lysine Modification

Position	Peptide	Type	References
119	RWLITLNKQQRRSDL	acetylation	[1]
119	RWLITLNKQQRRSDL	ubiquitination	[2]

Reference

 [1] Quantitative acetylome analysis reveals the roles of SIRT1 in regulating diverse substrates and cellular pathways.
 Chen Y, Zhao W, Yang JS, Cheng Z, Luo H, Lu Z, Tan M, Gu W, Zhao Y.
 Mol Cell Proteomics. 2012 Oct;11(10):1048-62. [PMID: 22826441]
 [2] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023] 

Functional Description

 Recognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in the initiation of protein synthesis and facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures. May play an important role in spermatogenesis through translational regulation of stage- specific mRNAs during germ cell development (By similarity). Its translation stimulation activity is repressed by binding to the complex CYFIP1-FMR1. Component of the CYFIP1-EIF4E-FMR1 complex which binds to the mRNA cap and mediates translational repression. In the CYFIP1-EIF4E-FMR1 complex this subunit mediates the binding to the mRNA cap. 

Sequence Annotation

 REGION 37 40 EIF4EBP1/2/3 binding.
 REGION 56 57 7-methylguanosine-containing mRNA cap
 REGION 73 77 EIF4EBP1/2/3 binding.
 REGION 102 103 7-methylguanosine-containing mRNA cap
 REGION 132 139 EIF4EBP1/2/3 binding.
 REGION 157 162 7-methylguanosine-containing mRNA cap
 REGION 205 207 7-methylguanosine-containing mRNA cap
 MOD_RES 2 2 N-acetylalanine (By similarity).
 MOD_RES 209 209 Phosphoserine; by PKC and MKNK2.  

Keyword

 3D-structure; Acetylation; Complete proteome; Cytoplasm; Initiation factor; Phosphoprotein; Protein biosynthesis; Reference proteome; RNA-binding; Translation regulation. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 217 AA 

Protein Sequence

Gene Ontology

 GO:0033391; C:chromatoid body; IDA:MGI.
 GO:0000932; C:cytoplasmic mRNA processing body; IEA:UniProtKB-SubCell.
 GO:0010494; C:cytoplasmic stress granule; ISS:UniProtKB.
 GO:0016281; C:eukaryotic translation initiation factor 4F complex; IEA:Compara.
 GO:0005845; C:mRNA cap binding complex; IDA:UniProtKB.
 GO:0016442; C:RNA-induced silencing complex; IEA:Compara.
 GO:0003743; F:translation initiation factor activity; IDA:UniProtKB.
 GO:0000082; P:G1/S transition of mitotic cell cycle; IEA:Compara.
 GO:0030324; P:lung development; IEA:Compara.
 GO:0045931; P:positive regulation of mitotic cell cycle; IEA:Compara.
 GO:0006417; P:regulation of translation; IEA:UniProtKB-KW. 

Interpro

 IPR023398; TIF_eIF4e-like_dom.
 IPR001040; TIF_eIF_4E.
 IPR019770; TIF_eIF_4E_CS. 

Pfam

 PF01652; IF4E 

SMART

  

PROSITE

 PS00813; IF4E 

PRINTS