CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-032186
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 6-phosphofructo-2-kinase 
Protein Synonyms/Alias
 cDNA FLJ54731, highly similar to 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 3 
Gene Name
 PFKFB3 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
125LAALRDVKSYLAKEGubiquitination[1, 2, 3]
130DVKSYLAKEGGQIAVubiquitination[4]
228DRDLSLIKVIDVGRRubiquitination[1, 2]
306FVEEQNLKDLRVWTSubiquitination[1, 2, 3, 4, 5, 6]
316RVWTSQLKSTIQTAEubiquitination[1, 2, 3, 4, 7, 8, 9, 10]
366YALREQDKYYYRYPTubiquitination[2]
Reference
 [1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [3] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [4] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [5] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [6] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
 [7] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [8] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [9] Proteome-wide identification of ubiquitylation sites by conjugation of engineered lysine-less ubiquitin.
 Oshikawa K, Matsumoto M, Oyamada K, Nakayama KI.
 J Proteome Res. 2012 Feb 3;11(2):796-807. [PMID: 22053931]
 [10] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789
Functional Description
  
Sequence Annotation
  
Keyword
 Complete proteome; Kinase; Reference proteome; Transferase. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 534 AA 
Protein Sequence
MGEGGQKEGD SQQAGALPLL CQLDTFSPKA TVFGVSINPA CGPKLTNSPT VIVMVGLPAR 60
GKTYISKKLT RYLNWIGVPT KVFNVGEYRR EAVKQYSSYN FFRPDNEEAM KVRKQCALAA 120
LRDVKSYLAK EGGQIAVFDA TNTTRERRHM ILHFAKENDF KAFFIESVCD DPTVVASNIM 180
EVKISSPDYK DCNSAEAMDD FMKRISCYEA SYQPLDPDKC DRDLSLIKVI DVGRRFLVNR 240
VQDHIQSRIV YYLMNIHVQP RTIYLCRHGE NEHNLQGRIG GDSGLSSRGK KFASALSKFV 300
EEQNLKDLRV WTSQLKSTIQ TAEALRLPYE QWKALNEIDA GVCEELTYEE IRDTYPEEYA 360
LREQDKYYYR YPTGESYQDL VQRLEPVIME LERQENVLVI CHQAVLRCLL AYFLDKSAEE 420
MPYLKCPLHT VLKLTPVAYG CRVESIYLNV ESVCTHRERS EDAKKGPNPL MRRNSVTPLA 480
SPEPTKKPRI NSFEEHVAST SAALPSCLPP EVPTQLPGQN MKGSRSSADS SRKH 534 
Gene Ontology
 GO:0003873; F:6-phosphofructo-2-kinase activity; IEA:InterPro.
 GO:0005524; F:ATP binding; IEA:InterPro.
 GO:0004331; F:fructose-2,6-bisphosphate 2-phosphatase activity; IEA:InterPro.
 GO:0006003; P:fructose 2,6-bisphosphate metabolic process; IEA:InterPro.
 GO:0006000; P:fructose metabolic process; IEA:InterPro. 
Interpro
 IPR003094; 6Pfruct_kin.
 IPR013079; 6Phosfructo_kin.
 IPR016260; Bifunct_6PFK/fruc_bisP_Ptase.
 IPR013078; His_Pase_superF_clade-1.
 IPR027417; P-loop_NTPase.
 IPR001345; PG/BPGM_mutase_AS. 
Pfam
 PF01591; 6PF2K
 PF00300; His_Phos_1 
SMART
 SM00855; PGAM 
PROSITE
 PS00175; PG_MUTASE 
PRINTS
 PR00991; 6PFRUCTKNASE.