CPLM-012444

Genbank Nucleotide ID

Condensin complex subunit 2

Protein Synonyms/Alias

Barren homolog protein 1; Chromosome-associated protein H; hCAP-H; Non-SMC condensin I complex subunit H; XCAP-H homolog

BRRN; BRRN1; CAPH; KIAA0074

Homo sapiens (Human)

Position	Peptide	Type	References
41	FPMPLPRKAPLNIPG	ubiquitination	[1]
122	IKLSTENKITTKNAF	ubiquitination	[2]
126	TENKITTKNAFGLHL	ubiquitination	[1]
142	DFMSEILKQKDTEPT	ubiquitination	[1]
144	MSEILKQKDTEPTNF	ubiquitination	[2]
152	DTEPTNFKVAAGTLD	ubiquitination	[2]
163	GTLDASTKIYAVRVD	ubiquitination	[2]
185	RVLGGLGKDAPSLEE	ubiquitination	[2]
210	TEMGTTKKAVKPKKK	ubiquitination	[2]
238	NVSEADRKCEIDPMF	ubiquitination	[2]
394	HEEFRSWKEPCQVQS	ubiquitination	[2]
424	MCPLLSMKPGEYSYF	ubiquitination	[2, 3]
488	DVYFRKTKAATILTK	ubiquitination	[2]
495	KAATILTKSTLENQN	ubiquitination	[4, 5]
532	KPGTRLLKMAQGHRV	ubiquitination	[2]
637	KIEIHYAKTAKKMDM	acetylation	[6]
637	KIEIHYAKTAKKMDM	ubiquitination	[2]
640	IHYAKTAKKMDMKKL	ubiquitination	[2]
648	KMDMKKLKQSMWSLL	ubiquitination	[2]
674	ANHREAGKEAALAEV	ubiquitination	[1, 2]
685	LAEVADEKMLSGLTK	ubiquitination	[2]
725	LANEKNLKLEGTEDL	ubiquitination	[1, 2]

[1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
[2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
[3] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
[4] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
[5] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
Chen Z, Zhou Y, Song J, Zhang Z.
Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
[6] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861]

Functional Description

Regulatory subunit of the condensin complex, a complex required for conversion of interphase chromatin into mitotic-like condense chromosomes. The condensin complex probably introduces positive supercoils into relaxed DNA in the presence of type I topoisomerases and converts nicked DNA into positive knotted forms in the presence of type II topoisomerases.

MOD_RES 15 15 Phosphoserine.
MOD_RES 25 25 Phosphoserine.
MOD_RES 49 49 Phosphothreonine.
MOD_RES 70 70 Phosphoserine.
MOD_RES 78 78 Phosphoserine.
MOD_RES 81 81 Phosphoserine.
MOD_RES 87 87 Phosphoserine.
MOD_RES 92 92 Phosphoserine.
MOD_RES 96 96 Phosphoserine.
MOD_RES 201 201 Phosphoserine.
MOD_RES 432 432 Phosphoserine.
MOD_RES 598 598 Phosphothreonine.
MOD_RES 605 605 Phosphothreonine.
MOD_RES 637 637 N6-acetyllysine.

Acetylation; Cell cycle; Cell division; Chromosome; Complete proteome; Cytoplasm; DNA condensation; Mitosis; Nucleus; Phosphoprotein; Polymorphism; Reference proteome.

UniProt (SWISSPROT/TrEMBL); GenBank; EMBL

GO:0000796; C:condensin complex; IDA:UniProtKB.
GO:0005829; C:cytosol; TAS:Reactome.
GO:0015630; C:microtubule cytoskeleton; IDA:HPA.
GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
GO:0051301; P:cell division; IEA:UniProtKB-KW.
GO:0000278; P:mitotic cell cycle; TAS:Reactome.
GO:0007076; P:mitotic chromosome condensation; IDA:UniProtKB.

IPR022816; Condensin_barren_su2.