UniProt Accession

 ACLY_RAT; P16638; Q497C7; Q8VIQ1 

Genbank Protein ID

 J05210; BC100618; AH011205 

Genbank Nucleotide ID

 AAA74463.1; AAI00619.1; AAL34316.1 

Protein Name

 ATP-citrate synthase 

Protein Synonyms/Alias

 ATP-citrate (pro-S-)-lyase; Citrate cleavage enzyme 

Gene Name

 Acly 

Gene Synonyms/Alias

  

Created Date

 July 27, 2013 

Organism

 Rattus norvegicus (Rat) 

NCBI Taxa ID

 10116 

Lysine Modification

Position	Peptide	Type	References
12	AISEQTGKELLYKYI	ubiquitination	[1]
68	QLIKRRGKLGLVGVN	ubiquitination	[1]
230	ATADYICKVKWGDIE	ubiquitination	[1]
259	YIADLDAKSGASLKL	ubiquitination	[1]
265	AKSGASLKLTLLNPK	ubiquitination	[1]
272	KLTLLNPKGRIWTMV	ubiquitination	[1]
537	YPFTGDHKQKFYWGH	ubiquitination	[1]
545	QKFYWGHKEILIPVF	ubiquitination	[1]
615	LIKKADQKGVTIIGP	ubiquitination	[1]
629	PATVGGIKPGCFKIG	ubiquitination	[1]
634	GIKPGCFKIGNTGGM	ubiquitination	[2]
649	LDNILASKLYRPGSV	ubiquitination	[1]
779	VAKNQALKEAGVFVP	ubiquitination	[1]
835	RELGLIRKPASFMTS	ubiquitination	[1]
917	IICARAGKDLVSSLT	ubiquitination	[1]
943	GALDAAAKMFSKAFD	ubiquitination	[1]
947	AAAKMFSKAFDSGII	ubiquitination	[1]
961	IPMEFVNKMKKEGKL	ubiquitination	[1]
977	MGIGHRVKSINNPDM	ubiquitination	[1]
990	DMRVQILKDFVKQHF	ubiquitination	[1]

Reference

 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023]
 [2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961] 

Functional Description

 ATP citrate-lyase is the primary enzyme responsible for the synthesis of cytosolic acetyl-CoA in many tissues. Has a central role in de novo lipid synthesis. In nervous tissue it may be involved in the biosynthesis of acetylcholine. 

Sequence Annotation

 NP_BIND 700 720 ATP (By similarity).
 NP_BIND 751 777 ATP (By similarity).
 REGION 778 788 CoA-binding (Potential).
 ACT_SITE 759 759 Tele-phosphohistidine intermediate.
 METAL 717 717 Magnesium (By similarity).
 BINDING 346 346 Citrate; via amide nitrogen (By
 BINDING 348 348 Citrate (By similarity).
 BINDING 379 379 Citrate (By similarity).
 MOD_RES 131 131 Phosphotyrosine (By similarity).
 MOD_RES 446 446 Phosphothreonine.
 MOD_RES 450 450 Phosphoserine.
 MOD_RES 454 454 Phosphoserine; by PKA and PKB/AKT1 or
 MOD_RES 480 480 Phosphoserine (By similarity).
 MOD_RES 545 545 N6-acetyllysine (By similarity).
 MOD_RES 553 553 N6-acetyllysine (By similarity).
 MOD_RES 638 638 Phosphothreonine (By similarity).
 MOD_RES 662 662 Phosphoserine (By similarity).
 MOD_RES 681 681 Phosphotyrosine (By similarity).
 MOD_RES 838 838 Phosphoserine (By similarity).
 MOD_RES 947 947 N6-acetyllysine (By similarity).
 MOD_RES 967 967 N6-acetyllysine (By similarity).
 MOD_RES 1076 1076 N6-acetyllysine (By similarity).
 MOD_RES 1099 1099 Phosphoserine (By similarity).  

Keyword

 Acetylation; Alternative splicing; ATP-binding; Complete proteome; Cytoplasm; Direct protein sequencing; Lipid biosynthesis; Lipid metabolism; Magnesium; Metal-binding; Nucleotide-binding; Phosphoprotein; Reference proteome; Transferase; Ubl conjugation. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 1100 AA 

Protein Sequence

Gene Ontology

 GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0003878; F:ATP citrate synthase activity; IDA:RGD.
 GO:0048037; F:cofactor binding; IEA:InterPro.
 GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
 GO:0004775; F:succinate-CoA ligase (ADP-forming) activity; IEA:InterPro.
 GO:0006085; P:acetyl-CoA biosynthetic process; NAS:RGD.
 GO:0044262; P:cellular carbohydrate metabolic process; IEA:InterPro.
 GO:0006101; P:citrate metabolic process; IDA:RGD.
 GO:0006633; P:fatty acid biosynthetic process; IDA:RGD. 

Interpro

 IPR014608; ATP-citrate_synthase.
 IPR013650; ATP-grasp_succ-CoA_synth-type.
 IPR013816; ATP_grasp_subdomain_2.
 IPR017440; Cit_synth/succinyl-CoA_lig_AS.
 IPR016143; Citrate_synth-like_sm_a-sub.
 IPR002020; Citrate_synthase-like.
 IPR016141; Citrate_synthase-like_core.
 IPR003781; CoA-bd.
 IPR005810; CoA_lig_alpha.
 IPR005811; CoA_ligase.
 IPR016040; NAD(P)-bd_dom.
 IPR017866; Succ-CoA_synthase_bsu_CS.
 IPR016102; Succinyl-CoA_synth-like. 

Pfam

 PF08442; ATP-grasp_2
 PF00285; Citrate_synt
 PF02629; CoA_binding
 PF00549; Ligase_CoA 

SMART

  

PROSITE

 PS01216; SUCCINYL_COA_LIG_1
 PS00399; SUCCINYL_COA_LIG_2
 PS01217; SUCCINYL_COA_LIG_3 

PRINTS