CPLM-021009

Genbank Nucleotide ID

Trans-2-enoyl-CoA reductase, mitochondrial

Protein Synonyms/Alias

Mus musculus (Mouse)

Position	Peptide	Type	References
56	GNHGDPAKVVQLKNL	acetylation	[1, 2]
61	PAKVVQLKNLELTAV	acetylation	[3, 4]
61	PAKVVQLKNLELTAV	succinylation	[4]
222	VRDRPDIKKLTDRLK	acetylation	[1]
229	KKLTDRLKDLGADYV	acetylation	[2]
248	ELRMPETKTIFKDLP	acetylation	[1, 4, 5, 6, 7]
248	ELRMPETKTIFKDLP	succinylation	[4]
252	PETKTIFKDLPLPRL	acetylation	[1, 2, 3, 4, 6, 8]
252	PETKTIFKDLPLPRL	succinylation	[4]
267	ALNCVGGKSSTELLR	acetylation	[1, 2, 4]
267	ALNCVGGKSSTELLR	succinylation	[4]
316	GFWLSQWKKNHSPDE	acetylation	[4]
316	GFWLSQWKKNHSPDE	succinylation	[4]
362	QALEASMKPFVSSKQ	acetylation	[2]

[1] Calorie restriction and SIRT3 trigger global reprogramming of the mitochondrial protein acetylome.
Hebert AS, Dittenhafer-Reed KE, Yu W, Bailey DJ, Selen ES, Boersma MD, Carson JJ, Tonelli M, Balloon AJ, Higbee AJ, Westphall MS, Pagliarini DJ, Prolla TA, Assadi-Porter F, Roy S, Denu JM, Coon JJ.
Mol Cell. 2013 Jan 10;49(1):186-99. [PMID: 23201123]
[2] Quantification of mitochondrial acetylation dynamics highlights prominent sites of metabolic regulation.
Still AJ, Floyd BJ, Hebert AS, Bingman CA, Carson JJ, Gunderson DR, Dolan BK, Grimsrud PA, Dittenhafer-Reed KE, Stapleton DS, Keller MP, Westphall MS, Denu JM, Attie AD, Coon JJ, Pagliarini DJ.
J Biol Chem. 2013 Jul 17;. [PMID: 23864654]
[3] Label-free quantitative proteomics of the lysine acetylome in mitochondria identifies substrates of SIRT3 in metabolic pathways.
Rardin MJ, Newman JC, Held JM, Cusack MP, Sorensen DJ, Li B, Schilling B, Mooney SD, Kahn CR, Verdin E, Gibson BW.
Proc Natl Acad Sci U S A. 2013 Apr 16;110(16):6601-6. [PMID: 23576753]
[4] SIRT5-Mediated Lysine Desuccinylation Impacts Diverse Metabolic Pathways.
Park J, Chen Y, Tishkoff DX, Peng C, Tan M, Dai L, Xie Z, Zhang Y, Zwaans BM, Skinner ME, Lombard DB, Zhao Y.
Mol Cell. 2013 Jun 27;50(6):919-30. [PMID: 23806337]
[5] Mitochondrial acetylome analysis in a mouse model of alcohol-induced liver injury utilizing SIRT3 knockout mice.
Fritz KS, Galligan JJ, Hirschey MD, Verdin E, Petersen DR.
J Proteome Res. 2012 Mar 2;11(3):1633-43. [PMID: 22309199]
[6] Quantitative acetylome analysis reveals the roles of SIRT1 in regulating diverse substrates and cellular pathways.
Chen Y, Zhao W, Yang JS, Cheng Z, Luo H, Lu Z, Tan M, Gu W, Zhao Y.
Mol Cell Proteomics. 2012 Oct;11(10):1048-62. [PMID: 22826441]
[7] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
PLoS One. 2012;7(12):e50545. [PMID: 23236377]
[8] Quantitative assessment of the impact of the gut microbiota on lysine epsilon-acetylation of host proteins using gnotobiotic mice.
Simon GM, Cheng J, Gordon JI.
Proc Natl Acad Sci U S A. 2012 Jul 10;109(28):11133-8. [PMID: 22733758]

Functional Description

Catalyzes the reduction of trans-2-enoyl-CoA to acyl-CoA with chain length from C6 to C16 in an NADPH-dependent manner with preference to medium chain length substrate. May have a role in the mitochondrial synthesis of fatty acids (By similarity).

Complete proteome; Fatty acid biosynthesis; Fatty acid metabolism; Lipid biosynthesis; Lipid metabolism; Mitochondrion; NADP; Oxidoreductase; Reference proteome; Transit peptide.

UniProt (SWISSPROT/TrEMBL); GenBank; EMBL

GO:0005829; C:cytosol; ISO:MGI.
GO:0005739; C:mitochondrion; IDA:MGI.
GO:0005634; C:nucleus; ISO:MGI.
GO:0016922; F:ligand-dependent nuclear receptor binding; ISO:MGI.
GO:0019166; F:trans-2-enoyl-CoA reductase (NADPH) activity; IEA:EC.
GO:0008270; F:zinc ion binding; IEA:InterPro.
GO:0006633; P:fatty acid biosynthetic process; IEA:UniProtKB-KW.

IPR013149; ADH_C.
IPR013154; ADH_GroES-like.
IPR002085; ADH_SF_Zn-type.
IPR011032; GroES-like.
IPR016040; NAD(P)-bd_dom.

PF08240; ADH_N
PF00107; ADH_zinc_N