CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-013466
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Rho GTPase-activating protein 15 
Protein Synonyms/Alias
 ArhGAP15; Rho-type GTPase-activating protein 15 
Gene Name
 ARHGAP15 
Gene Synonyms/Alias
 BM-024; BM-030; BM-046 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
51MILTDVGKVTEPISRubiquitination[1]
69NHSQHILKDVIPPLEubiquitination[1]
82LEQLMVEKEGYLQKAubiquitination[1]
88EKEGYLQKAKIADGGubiquitination[1]
118SRRIEFYKESKQQALacetylation[2]
118SRRIEFYKESKQQALubiquitination[1]
121IEFYKESKQQALSNMubiquitination[1]
129QQALSNMKTGHKPESubiquitination[1]
133SNMKTGHKPESVDLCubiquitination[1]
206SRNLELFKIQRSSSTubiquitination[1, 3]
232EQKPEHRKSLMFRLHubiquitination[1]
266ITRRPSLKTLQEKGLubiquitination[1]
271SLKTLQEKGLIKDQIubiquitination[1, 4]
275LQEKGLIKDQIFGSHubiquitination[1]
285IFGSHLHKVCERENSubiquitination[1]
299STVPWFVKQCIEAVEubiquitination[1]
307QCIEAVEKRGLDVDGubiquitination[1]
327GNLATIQKLRFIVNQubiquitination[1]
392NTRIEAVKSLVQKLPubiquitination[1]
415VLFGHLTKIVAKASKubiquitination[1]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
 Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
 Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861]
 [3] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [4] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302] 
Functional Description
 GTPase activator for the Rho-type GTPases by converting them to an inactive GDP-bound state. Has activity toward RAC1. Overexpression results in an increase in actin stress fibers and cell contraction. 
Sequence Annotation
 DOMAIN 79 189 PH.
 DOMAIN 281 470 Rho-GAP.
 MOD_RES 43 43 Phosphoserine.
 MOD_RES 103 103 Phosphoserine.  
Keyword
 3D-structure; Complete proteome; Cytoplasm; GTPase activation; Membrane; Phosphoprotein; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 475 AA 
Protein Sequence
MQKSTNSDTS VETLNSTRQG TGAVQMRIKN ANSHHDRLSQ SKSMILTDVG KVTEPISRHR 60
RNHSQHILKD VIPPLEQLMV EKEGYLQKAK IADGGKKLRK NWSTSWIVLS SRRIEFYKES 120
KQQALSNMKT GHKPESVDLC GAHIEWAKEK SSRKNVFQIT TVSGNEFLLQ SDIDFIILDW 180
FHAIKNAIDR LPKDSSCPSR NLELFKIQRS SSTELLSHYD SDIKEQKPEH RKSLMFRLHH 240
SASDTSDKNR VKSRLKKFIT RRPSLKTLQE KGLIKDQIFG SHLHKVCERE NSTVPWFVKQ 300
CIEAVEKRGL DVDGIYRVSG NLATIQKLRF IVNQEEKLNL DDSQWEDIHV VTGALKMFFR 360
ELPEPLFPYS FFEQFVEAIK KQDNNTRIEA VKSLVQKLPP PNRDTMKVLF GHLTKIVAKA 420
SKNLMSTQSL GIVFGPTLLR AENETGNMAI HMVYQNQIAE LMLSEYSKIF GSEED 475 
Gene Ontology
 GO:0005829; C:cytosol; TAS:Reactome.
 GO:0016020; C:membrane; IEA:UniProtKB-SubCell.
 GO:0005543; F:phospholipid binding; IEA:InterPro.
 GO:0030675; F:Rac GTPase activator activity; IDA:MGI.
 GO:0008360; P:regulation of cell shape; IDA:MGI.
 GO:0007264; P:small GTPase mediated signal transduction; TAS:Reactome. 
Interpro
 IPR011993; PH_like_dom.
 IPR001849; Pleckstrin_homology.
 IPR015767; Rho_GTPase_activating.
 IPR008936; Rho_GTPase_activation_prot.
 IPR000198; RhoGAP_dom. 
Pfam
 PF00169; PH
 PF00620; RhoGAP 
SMART
 SM00233; PH
 SM00324; RhoGAP 
PROSITE
 PS50003; PH_DOMAIN
 PS50238; RHOGAP 
PRINTS