CPLM-006658

Genbank Nucleotide ID

7,8-dihydro-8-oxoguanine triphosphatase

Protein Synonyms/Alias

2-hydroxy-dATP diphosphatase; 8-oxo-dGTPase; Nucleoside diphosphate-linked moiety X motif 1; Nudix motif 1

Homo sapiens (Human)

Position	Peptide	Type	References
79	RWNGFGGKVQEGETI	acetylation	[1]
79	RWNGFGGKVQEGETI	ubiquitination	[2, 3, 4, 5]

[1] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
PLoS One. 2012;7(12):e50545. [PMID: 23236377]
[2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
[3] Global identification of modular cullin-RING ligase substrates.
Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
[4] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
[5] Integrated proteomic analysis of post-translational modifications by serial enrichment.
Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]

Functional Description

Antimutagenic. Acts as a sanitizing enzyme for oxidized nucleotide pools, thus suppressing cell dysfunction and death induced by oxidative stress. Hydrolyzes 8-oxo-dGTP, 8-oxo-dATP and 2-OH-dATP, thus preventing misincorporation of oxidized purine nucleoside triphosphates into DNA and subsequently preventing A:T to C:G and G:C to T:A transversions. Able to hydrolyze also the corresponding ribonucleotides, 2-OH-ATP, 8-oxo-GTP and 8-oxo-ATP. Does not play a role in U8 snoRNA decapping activity. Binds U8 snoRNA.

DOMAIN 44 173 Nudix hydrolase.
REGION 76 79 Substrate binding (Probable).
REGION 158 161 Substrate binding.
MOTIF 78 99 Nudix box.
METAL 78 78 Magnesium; via carbonyl oxygen (By
METAL 93 93 Magnesium (By similarity).
METAL 96 96 Magnesium (By similarity).
METAL 97 97 Magnesium (By similarity).
BINDING 49 49 Substrate; via carbonyl oxygen.
BINDING 68 68 Substrate.
BINDING 74 74 Substrate.

3D-structure; Alternative initiation; Complete proteome; Cytoplasm; Direct protein sequencing; Hydrolase; Magnesium; Metal-binding; Mitochondrion; Nucleus; Polymorphism; Reference proteome; RNA-binding; Transit peptide.

UniProt (SWISSPROT/TrEMBL); GenBank; EMBL

GO:0005829; C:cytosol; TAS:Reactome.
GO:0005759; C:mitochondrial matrix; IDA:UniProtKB.
GO:0005634; C:nucleus; IDA:UniProtKB.
GO:0035539; F:8-oxo-7,8-dihydrodeoxyguanosine triphosphate pyrophosphatase activity; IDA:UniProtKB.
GO:0008413; F:8-oxo-7,8-dihydroguanosine triphosphate pyrophosphatase activity; IDA:UniProtKB.
GO:0047693; F:ATP diphosphatase activity; IDA:UniProtKB.
GO:0003924; F:GTPase activity; TAS:ProtInc.
GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
GO:0030515; F:snoRNA binding; ISS:UniProtKB.
GO:0006200; P:ATP catabolic process; IDA:UniProtKB.
GO:0006203; P:dGTP catabolic process; IDA:UniProtKB.
GO:0042262; P:DNA protection; IDA:UniProtKB.
GO:0006281; P:DNA repair; IEA:InterPro.
GO:0034656; P:nucleobase-containing small molecule catabolic process; TAS:Reactome.
GO:0006979; P:response to oxidative stress; TAS:ProtInc.

IPR020476; Nudix_hydrolase.
IPR020084; NUDIX_hydrolase_CS.
IPR000086; NUDIX_hydrolase_dom.
IPR015797; NUDIX_hydrolase_dom-like.
IPR003563; OxG-triPHTase.

PS51462; NUDIX
PS00893; NUDIX_BOX

PR01403; 8OXTPHPHTASE.
PR00502; NUDIXFAMILY.