CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-023225
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Nucleolar protein 7 
Protein Synonyms/Alias
 Nucleolar protein of 27 kDa 
Gene Name
 NOL7 
Gene Synonyms/Alias
 C6orf90; NOP27 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
170VQSVSQNKSYLAVRLubiquitination[1, 2, 3]
210TNRTTVNKFLSLANKubiquitination[4, 5, 6]
Reference
 [1] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [3] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [4] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [5] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
 [6] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789
Functional Description
  
Sequence Annotation
 MOD_RES 133 133 Phosphoserine (By similarity).  
Keyword
 Alternative splicing; Coiled coil; Complete proteome; Nucleus; Phosphoprotein; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 257 AA 
Protein Sequence
MVQLRPRASR APASAEAMVD EGQLASEEEE AEHGLLLGQP SSGAAAEPLE EDEEGDDEFD 60
DEAPEELTFA SAQAEAREEE RRVRETVRRD KTLLKEKRKR REELFIEQKK RKLLPDTILE 120
KLTTASQTNI KKSPGKVKEV NLQKKNEDCE KGNDSKKVKV QKVQSVSQNK SYLAVRLKDQ 180
DLRDSRQQAA QAFIHNSLYG PGTNRTTVNK FLSLANKRLP VKRAAVQFLN NAWGIQKKQN 240
AKRFKRRWMV RKMKTKK 257 
Gene Ontology
 GO:0005739; C:mitochondrion; IDA:HPA.
 GO:0005730; C:nucleolus; IDA:HPA. 
Interpro
 IPR012579; NUC129. 
Pfam
 PF08157; NUC129 
SMART
  
PROSITE
  
PRINTS