CPLM-001217

Genbank Nucleotide ID

Barrier-to-autointegration factor

Protein Synonyms/Alias

Breakpoint cluster region protein 1; Barrier-to-autointegration factor, N-terminally processed

Homo sapiens (Human)

Position	Peptide	Type	References
32	GIGEVLGKKLEERGF	ubiquitination	[1, 2]
41	LEERGFDKAYVVLGQ	ubiquitination	[3]
54	GQFLVLKKDEDLFRE	ubiquitination	[4]
64	DLFREWLKDTCGANA	acetylation	[4, 5, 6]
64	DLFREWLKDTCGANA	ubiquitination	[4]
72	DTCGANAKQSRDCFG	ubiquitination	[7, 8]

[1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
[2] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
Chen Z, Zhou Y, Song J, Zhang Z.
Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
[3] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
[4] Integrated proteomic analysis of post-translational modifications by serial enrichment.
Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
[5] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861]
[6] Proteomic investigations reveal a role for RNA processing factor THRAP3 in the DNA damage response.
Beli P, Lukashchuk N, Wagner SA, Weinert BT, Olsen JV, Baskcomb L, Mann M, Jackson SP, Choudhary C.
Mol Cell. 2012 Apr 27;46(2):212-25. [PMID: 22424773]
[7] Systematic and quantitative assessment of the ubiquitin-modified proteome.
Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
[8] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]

Functional Description

Plays fundamental roles in nuclear assembly, chromatin organization, gene expression and gonad development. May potently compress chromatin structure and be involved in membrane recruitment and chromatin decondensation during nuclear assembly. Contains 2 non-specific dsDNA-binding sites which may promote DNA cross-bridging. Exploited by retroviruses for inhibiting self- destructing autointegration of retroviral DNA, thereby promoting integration of viral DNA into the host chromosome. EMD and BAF are cooperative cofactors of HIV-1 infection. Association of EMD with the viral DNA requires the presence of BAF and viral integrase. The association of viral DNA with chromatin requires the presence of BAF and EMD.

MOD_RES 1 1 N-acetylmethionine.
MOD_RES 2 2 N-acetylthreonine; in Barrier-to-
MOD_RES 2 2 Phosphothreonine; by VRK1 and VRK2.
MOD_RES 3 3 Phosphothreonine; by VRK1 and VRK2.
MOD_RES 4 4 Phosphoserine; by VRK1 and VRK2.

3D-structure; Acetylation; Chromosome; Complete proteome; Cytoplasm; Disease mutation; DNA-binding; Host-virus interaction; Nucleus; Phosphoprotein; Reference proteome.

UniProt (SWISSPROT/TrEMBL); GenBank; EMBL

GO:0005694; C:chromosome; IEA:UniProtKB-SubCell.
GO:0005829; C:cytosol; TAS:Reactome.
GO:0005654; C:nucleoplasm; TAS:Reactome.
GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
GO:0015074; P:DNA integration; IEA:Compara.
GO:0075713; P:establishment of integrated proviral latency; TAS:Reactome.
GO:0007077; P:mitotic nuclear envelope disassembly; TAS:Reactome.
GO:0007084; P:mitotic nuclear envelope reassembly; TAS:Reactome.
GO:0009615; P:response to virus; TAS:ProtInc.

IPR004122; BAF_prot.