UniProt Accession

 HIRA_HUMAN; P54198; Q05BU9; Q8IXN2 

Genbank Protein ID

 X81844; X77633; X89887; X91501; CR456503; BC032721; BC039835; X75296 

Genbank Nucleotide ID

 CAA57436.1; CAA54721.1; CAA61979.1; CAA62800.1; CAG30389.1; AAH32721.1; AAH39835.1; CAA53044.1 

Protein Name

 Protein HIRA 

Protein Synonyms/Alias

 TUP1-like enhancer of split protein 1 

Gene Name

 HIRA 

Gene Synonyms/Alias

 DGCR1; HIR; TUPLE1 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
27	DIHPDGTKFATGGQG	ubiquitination	[1, 2]
114	TVFGSSGKLANVEQW	ubiquitination	[3]
178	RGHSGLVKGLTWDPV	ubiquitination	[1, 2, 3]
187	LTWDPVGKYIASQAD	ubiquitination	[3, 4]
214	QLETSITKPFDECGG	ubiquitination	[3]
259	IIEREGWKTNMDFVG	ubiquitination	[3]
275	RKAVTVVKFNPKIFK	ubiquitination	[3]
279	TVVKFNPKIFKKKQK	ubiquitination	[3]
292	QKNGSSAKPSCPYCC	ubiquitination	[3]
381	IHQSTYGKSLAIMTE	ubiquitination	[3]
416	QQQQLDQKSAATREM	ubiquitination	[3]
728	LKCNREGKEWETVLT	ubiquitination	[3]
754	VVCVACEKRMLSVFS	ubiquitination	[3]
860	TWNLVSDKQDSLAQC	ubiquitination	[3]
988	LRKRELLKELLPVIG	ubiquitination	[3]

Reference

 [1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [2] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [3] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [4] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983] 

Functional Description

 Cooperates with ASF1A to promote replication-independent chromatin assembly. Required for the periodic repression of histone gene transcription during the cell cycle. Required for the formation of senescence-associated heterochromatin foci (SAHF) and efficient senescence-associated cell cycle exit. 

Sequence Annotation

 REPEAT 11 53 WD 1.
 REPEAT 68 107 WD 2.
 REPEAT 129 168 WD 3.
 REPEAT 172 211 WD 4.
 REPEAT 220 263 WD 5.
 REPEAT 266 322 WD 6.
 REPEAT 326 367 WD 7.
 REPEAT 772 809 WD 8.
 REGION 421 729 Interaction with CCNA1.
 REGION 421 479 Interaction with ASF1A.
 REGION 439 475 Required for repression of histone gene
 REGION 593 826 Interaction with histone H2B.
 REGION 594 739 Interaction with PAX3 (By similarity).
 REGION 738 1017 Interaction with histone H4.
 REGION 740 828 Interaction with PAX3 (By similarity).
 MOD_RES 549 549 Phosphoserine.
 MOD_RES 555 555 Phosphothreonine; by CDK2.
 MOD_RES 557 557 Phosphoserine.
 MOD_RES 576 576 Phosphothreonine.
 MOD_RES 584 584 Phosphoserine.
 MOD_RES 586 586 Phosphothreonine.
 MOD_RES 610 610 Phosphoserine.
 MOD_RES 611 611 Phosphoserine.
 MOD_RES 612 612 Phosphoserine.
 MOD_RES 614 614 Phosphoserine.
 MOD_RES 661 661 Phosphoserine.
 MOD_RES 687 687 Phosphoserine.  

Keyword

 3D-structure; Alternative splicing; Chromatin regulator; Complete proteome; Nucleus; Phosphoprotein; Reference proteome; Repeat; Repressor; Transcription; Transcription regulation; Ubl conjugation; WD repeat. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 1017 AA 

Protein Sequence

Gene Ontology

 GO:0000790; C:nuclear chromatin; IEA:Compara.
 GO:0005634; C:nucleus; TAS:ProtInc.
 GO:0016605; C:PML body; IEA:UniProtKB-SubCell.
 GO:0003682; F:chromatin binding; IEA:InterPro.
 GO:0003700; F:sequence-specific DNA binding transcription factor activity; TAS:ProtInc.
 GO:0003714; F:transcription corepressor activity; TAS:ProtInc.
 GO:0009653; P:anatomical structure morphogenesis; TAS:ProtInc.
 GO:0016568; P:chromatin modification; IEA:UniProtKB-KW.
 GO:0007369; P:gastrulation; IEA:Compara.
 GO:0042692; P:muscle cell differentiation; IEA:Compara.
 GO:0001649; P:osteoblast differentiation; IEA:Compara.
 GO:0006357; P:regulation of transcription from RNA polymerase II promoter; TAS:ProtInc.
 GO:0006351; P:transcription, DNA-dependent; IEA:UniProtKB-KW. 

Interpro

 IPR011494; Hira.
 IPR019015; HIRA_B_motif.
 IPR015943; WD40/YVTN_repeat-like_dom.
 IPR001680; WD40_repeat.
 IPR019775; WD40_repeat_CS.
 IPR017986; WD40_repeat_dom. 

Pfam

 PF07569; Hira
 PF09453; HIRA_B
 PF00400; WD40 

SMART

 SM00320; WD40 

PROSITE

 PS00678; WD_REPEATS_1
 PS50082; WD_REPEATS_2
 PS50294; WD_REPEATS_REGION 

PRINTS