CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-019504
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Crossover junction endonuclease MUS81 
Protein Synonyms/Alias
  
Gene Name
 MUS81 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
45RTRFVFQKALRSLRRubiquitination[1]
64LRSGKEAKILQHFGDubiquitination[1]
123MPVPAQPKAGGSGSYubiquitination[1]
335LDHIVERKRLDDLCSubiquitination[1]
406VKRTADIKESAAYLAubiquitination[1]
489VRGVSGEKAAALVDRubiquitination[1]
524ETLLSTIKCGRLQRNubiquitination[1]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961] 
Functional Description
 Interacts with EME1 and EME2 to form a DNA structure- specific endonuclease with substrate preference for branched DNA structures with a 5'-end at the branch nick. Typical substrates include 3'-flap structures, replication forks and nicked Holliday junctions. May be required in mitosis for the processing of stalled or collapsed replication forks. 
Sequence Annotation
 DOMAIN 270 372 ERCC4.
 REGION 125 244 Interaction with BLM.  
Keyword
 3D-structure; Complete proteome; DNA damage; DNA recombination; DNA repair; Endonuclease; Hydrolase; Magnesium; Metal-binding; Nuclease; Nucleus; Polymorphism; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 551 AA 
Protein Sequence
MAAPVRLGRK RPLPACPNPL FVRWLTEWRD EATRSRRRTR FVFQKALRSL RRYPLPLRSG 60
KEAKILQHFG DGLCRMLDER LQRHRTSGGD HAPDSPSGEN SPAPQGRLAE VQDSSMPVPA 120
QPKAGGSGSY WPARHSGARV ILLVLYREHL NPNGHHFLTK EELLQRCAQK SPRVAPGSAR 180
PWPALRSLLH RNLVLRTHQP ARYSLTPEGL ELAQKLAESE GLSLLNVGIG PKEPPGEETA 240
VPGAASAELA SEAGVQQQPL ELRPGEYRVL LCVDIGETRG GGHRPELLRE LQRLHVTHTV 300
RKLHVGDFVW VAQETNPRDP ANPGELVLDH IVERKRLDDL CSSIIDGRFR EQKFRLKRCG 360
LERRVYLVEE HGSVHNLSLP ESTLLQAVTN TQVIDGFFVK RTADIKESAA YLALLTRGLQ 420
RLYQGHTLRS RPWGTPGNPE SGAMTSPNPL CSLLTFSDFN AGAIKNKAQS VREVFARQLM 480
QVRGVSGEKA AALVDRYSTP ASLLAAYDAC ATPKEQETLL STIKCGRLQR NLGPALSRTL 540
SQLYCSYGPL T 551 
Gene Ontology
 GO:0005730; C:nucleolus; IEA:UniProtKB-SubCell.
 GO:0048257; F:3'-flap endonuclease activity; IMP:UniProtKB.
 GO:0003677; F:DNA binding; IEA:InterPro.
 GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
 GO:0006310; P:DNA recombination; IEA:UniProtKB-KW.
 GO:0006281; P:DNA repair; IMP:UniProtKB. 
Interpro
 IPR010996; DNA_pol_b-like_N.
 IPR020819; DNA_repair_nuc_XPF/helicase.
 IPR006166; ERCC4_domain.
 IPR003583; Hlx-hairpin-Hlx_DNA-bd_motif.
 IPR027420; PolB_N.
 IPR011335; Restrct_endonuc-II-like. 
Pfam
 PF02732; ERCC4 
SMART
 SM00891; ERCC4
 SM00278; HhH1 
PROSITE
  
PRINTS