CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-031190
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Poly(A)-specific ribonuclease PARN 
Protein Synonyms/Alias
 cDNA FLJ58618, highly similar to Poly(A)-specific ribonuclease PARN (EC 3.1.13.4) 
Gene Name
 PARN 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
84REQQKHAKEQEELNDubiquitination[1]
151FPRLLDTKLMASTQPubiquitination[1, 2, 3]
238IHVSARSKLIEPFFNubiquitination[1, 4]
246LIEPFFNKLFLMRVMubiquitination[1]
324KIAVNTSKYAESYRIacetylation[4, 5, 6]
324KIAVNTSKYAESYRIubiquitination[1]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [3] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [4] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [5] Proteomic investigations reveal a role for RNA processing factor THRAP3 in the DNA damage response.
 Beli P, Lukashchuk N, Wagner SA, Weinert BT, Olsen JV, Baskcomb L, Mann M, Jackson SP, Choudhary C.
 Mol Cell. 2012 Apr 27;46(2):212-25. [PMID: 22424773]
 [6] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
 Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
 PLoS One. 2012;7(12):e50545. [PMID: 23236377] 
Functional Description
  
Sequence Annotation
  
Keyword
 Complete proteome; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 464 AA 
Protein Sequence
MEIIRSNFKS NLHKVYQAIE EADFFAIDGE FSGISDGPSV SALTNGFDTP EERYQKLKKK 60
ERYIVISKVD EEERKRREQQ KHAKEQEELN DAVGFSRVIH AIANSGKLVI GHNMLLDVMH 120
TVHQFYCPLP ADLSEFKEMT TCVFPRLLDT KLMASTQPFK DIINNTSLAE LEKRLKETPF 180
NPPKVESAEG FPSYDTASEQ LHEAGYDAYI TGLCFISMAN YLGSFLSPPK IHVSARSKLI 240
EPFFNKLFLM RVMDIPYLNL EGPDLQPKRD HVLHVTFPKE WKTSDLYQLF SAFGNIQISW 300
IDDTSAFVSL SQPEQVKIAV NTSKYAESYR IQTYAEYMGR KQEEKQIKRK WTEDSWKEAD 360
SKRLNPQCIP YTLQNHYYRN NSFTAPSTVG KRNLSPSQEE AGLEDGVSGE ISDTELEQTD 420
SCAEPLSEGR KKAKKLKRMK KELSPAGSIS KNSPATLFEV PDTW 464 
Gene Ontology
 GO:0005737; C:cytoplasm; IEA:InterPro.
 GO:0005634; C:nucleus; IEA:InterPro.
 GO:0046872; F:metal ion binding; IEA:InterPro.
 GO:0000166; F:nucleotide binding; IEA:InterPro.
 GO:0004535; F:poly(A)-specific ribonuclease activity; IEA:InterPro.
 GO:0003723; F:RNA binding; IEA:InterPro.
 GO:0006402; P:mRNA catabolic process; IEA:InterPro. 
Interpro
 IPR012677; Nucleotide-bd_a/b_plait.
 IPR014789; PolyA-riboNase_RNA_binding.
 IPR006941; RNase_CAF1.
 IPR012337; RNaseH-like_dom. 
Pfam
 PF04857; CAF1
 PF08675; RNA_bind 
SMART
  
PROSITE
  
PRINTS