UniProt Accession

 H13_HUMAN; P16402; B2R751; Q2M2I2 

Genbank Protein ID

 M60747; AF531303; AK312845; AL031777; CH471087; BC104874; BC111971 

Genbank Nucleotide ID

 AAA63186.1; AAN06703.1; BAG35698.1; CAB39189.1; EAW55548.1; AAI04875.1; AAI11972.1 

Protein Name

 Histone H1.3 

Protein Synonyms/Alias

 Histone H1c; Histone H1s-2 

Gene Name

 HIST1H1D 

Gene Synonyms/Alias

 H1F3 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
17	TIPAPAEKTPVKKKA	acetylation	[1, 2, 3]
17	TIPAPAEKTPVKKKA	ubiquitination	[4, 5]
21	PAEKTPVKKKAKKAG	ubiquitination	[3, 4, 5]
22	AEKTPVKKKAKKAGA	ubiquitination	[5]
35	GATAGKRKASGPPVS	acetylation	[1]
35	GATAGKRKASGPPVS	glycation	[6]
47	PVSELITKAVAASKE	acetylation	[1]
47	PVSELITKAVAASKE	glycation	[6]
47	PVSELITKAVAASKE	ubiquitination	[1]
53	TKAVAASKERSGVSL	acetylation	[1]
65	VSLAALKKALAAAGY	acetylation	[1]
86	SRIKLGLKSLVSKGT	acetylation	[1]
91	GLKSLVSKGTLVQTK	acetylation	[1]
98	KGTLVQTKGTGASGS	acetylation	[1]
169	PATAAGTKKVAKSAK	acetylation	[1]

Reference

 [1] Mass spectrometric mapping of linker histone H1 variants reveals multiple acetylations, methylations, and phosphorylation as well as differences between cell culture and tissue.
 Wisniewski JR, Zougman A, Krüger S, Mann M.
 Mol Cell Proteomics. 2007 Jan;6(1):72-87. [PMID: 17043054]
 [2] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
 Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
 Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861]
 [3] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [4] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [5] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [6] Ex vivo detection of histone H1 modified with advanced glycation end products.
 Pashikanti S, Boissonneault GA, Cervantes-Laurean D.
 Free Radic Biol Med. 2011 May 15;50(10):1410-6. [PMID: 21315148] 

Functional Description

 Histone H1 protein binds to linker DNA between nucleosomes forming the macromolecular structure known as the chromatin fiber. Histones H1 are necessary for the condensation of nucleosome chains into higher-order structured fibers. Acts also as a regulator of individual gene transcription through chromatin remodeling, nucleosome spacing and DNA methylation (By similarity). 

Sequence Annotation

 DOMAIN 37 110 H15.
 MOD_RES 2 2 N-acetylserine.
 MOD_RES 2 2 Phosphoserine.
 MOD_RES 18 18 Phosphothreonine.
 MOD_RES 37 37 Phosphoserine (By similarity).
 MOD_RES 105 105 Phosphoserine; by PKC (By similarity).  

Keyword

 Acetylation; Chromosome; Complete proteome; Direct protein sequencing; DNA-binding; Nucleus; Phosphoprotein; Polymorphism; Reference proteome. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 221 AA 

Protein Sequence

Gene Ontology

 GO:0000790; C:nuclear chromatin; IDA:UniProtKB.
 GO:0000786; C:nucleosome; NAS:UniProtKB.
 GO:0003677; F:DNA binding; NAS:UniProtKB.
 GO:0006334; P:nucleosome assembly; NAS:UniProtKB.
 GO:0016584; P:nucleosome positioning; IEA:Compara. 

Interpro

 IPR005818; Histone_H1/H5.
 IPR005819; Histone_H5.
 IPR011991; WHTH_DNA-bd_dom. 

Pfam

 PF00538; Linker_histone 

SMART

 SM00526; H15 

PROSITE

 PS51504; H15 

PRINTS

 PR00624; HISTONEH5.