UniProt Accession

 GATY_ECOLI; P0C8J6; P37192; P76415 

Genbank Protein ID

 U00096; AP009048 

Genbank Nucleotide ID

 AAC75157.2; BAA15966.2 

Protein Name

 D-tagatose-1,6-bisphosphate aldolase subunit GatY 

Protein Synonyms/Alias

 TBPA; TagBP aldolase; D-tagatose-bisphosphate aldolase class II; Tagatose-bisphosphate aldolase 

Gene Name

 gatY 

Gene Synonyms/Alias

 yegF; b2096; JW5343 

Created Date

 July 27, 2013 

Organism

 Escherichia coli (strain K12) 

NCBI Taxa ID

 83333 

Lysine Modification

Position	Peptide	Type	References
86	IHLDHHTKFDDIAQK	acetylation	[1, 2]
93	KFDDIAQKVRSGVRS	acetylation	[2]
118	AQNISRVKEVVDFCH	acetylation	[1]
216	GASGLSTKDIQQTIK	acetylation	[1, 2]
223	KDIQQTIKLGICKIN	acetylation	[1, 2]
236	INVATELKNAFSQAL	acetylation	[1, 2]
264	RDYLQSAKSAMRDVV	acetylation	[2]
273	AMRDVVSKVIADCGC	acetylation	[1]

Reference

 [1] Comprehensive profiling of protein lysine acetylation in Escherichia coli.
 Zhang K, Zheng S, Yang JS, Chen Y, Cheng Z.
 J Proteome Res. 2013 Feb 1;12(2):844-51. [PMID: 23294111]
 [2] Acetyl-Phosphate Is a Critical Determinant of Lysine Acetylation in E. coli.
 Weinert BT, Iesmantavicius V, Wagner SA, Schölz C, Gummesson B, Beli P, Nyström T, Choudhary C.
 Mol Cell. 2013 Jul 25;51(2):265-72. [PMID: 23830618] 

Functional Description

 Catalytic subunit of the tagatose-1,6-bisphosphate aldolase GatYZ, which catalyzes the reversible aldol condensation of dihydroxyacetone phosphate (DHAP or glycerone-phosphate) with glyceraldehyde 3-phosphate (G3P) to produce tagatose 1,6- bisphosphate (TBP). Requires GatZ subunit for full activity and stability. Is involved in the catabolism of galactitol. 

Sequence Annotation

 REGION 209 211 Dihydroxyacetone phosphate binding (By
 REGION 230 233 Dihydroxyacetone phosphate binding (By
 ACT_SITE 82 82 Proton donor (By similarity).
 METAL 83 83 Zinc; catalytic (By similarity).
 METAL 180 180 Zinc; catalytic (By similarity).
 METAL 208 208 Zinc; catalytic (By similarity).
 BINDING 181 181 Dihydroxyacetone phosphate; via amide  

Keyword

 Complete proteome; Direct protein sequencing; Galactitol metabolism; Lyase; Metal-binding; Reference proteome; Zinc. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 284 AA 

Protein Sequence

Gene Ontology

 GO:0009025; F:tagatose-bisphosphate aldolase activity; IEA:HAMAP.
 GO:0008270; F:zinc ion binding; IEA:HAMAP.
 GO:2001059; P:D-tagatose 6-phosphate catabolic process; IEA:UniProtKB-UniPathway.
 GO:0019404; P:galactitol catabolic process; IEA:HAMAP. 

Interpro

 IPR013785; Aldolase_TIM.
 IPR000771; Ketose_bisP_aldolase_II.
 IPR011288; TagBP_ald_KbaY/GatY.
 IPR023955; TagBP_aldolase_GatY. 

Pfam

 PF01116; F_bP_aldolase 

SMART

  

PROSITE

 PS00602; ALDOLASE_CLASS_II_1
 PS00806; ALDOLASE_CLASS_II_2 

PRINTS