UniProt Accession

 PDX3_YEAST; P38075; D6VQ35 

Genbank Protein ID

 X76078; X76992; Z35904; AY557712; BK006936 

Genbank Nucleotide ID

 CAA53690.1; CAA54295.1; CAA84977.1; AAS56038.1; DAA07155.1 

Protein Name

 Pyridoxamine 5'-phosphate oxidase 

Protein Synonyms/Alias

 PNP/PMP oxidase; PNPOx 

Gene Name

 PDX3 

Gene Synonyms/Alias

 YBR035C; YBR0321 

Created Date

 July 27, 2013 

Organism

 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 

NCBI Taxa ID

 559292 

Lysine Modification

Position	Peptide	Type	References
10	KQAEETQKPIIFAPE	acetylation	[1]
29	DKFTLNEKQLTDDPI	acetylation	[1]
29	DKFTLNEKQLTDDPI	ubiquitination	[2, 3, 4]
78	SSRILLFKELDHRGF	acetylation	[1]
158	SRQSDVIKNREELDE	acetylation	[1]

Reference

 [1] Proteome-wide analysis of lysine acetylation suggests its broad regulatory scope in Saccharomyces cerevisiae.
 Henriksen P, Wagner SA, Weinert BT, Sharma S, Bacinskaja G, Rehman M, Juffer AH, Walther TC, Lisby M, Choudhary C.
 Mol Cell Proteomics. 2012 Nov;11(11):1510-22. [PMID: 22865919]
 [2] A proteomics approach to understanding protein ubiquitination.
 Peng J, Schwartz D, Elias JE, Thoreen CC, Cheng D, Marsischky G, Roelofs J, Finley D, Gygi SP.
 Nat Biotechnol. 2003 Aug;21(8):921-6. [PMID: 12872131]
 [3] Computational identification of ubiquitylation sites from protein sequences.
 Tung CW, Ho SY.
 BMC Bioinformatics. 2008 Jul 15;9:310. [PMID: 18625080]
 [4] Identification, analysis, and prediction of protein ubiquitination sites.
 Radivojac P, Vacic V, Haynes C, Cocklin RR, Mohan A, Heyen JW, Goebl MG, Iakoucheva LM.
 Proteins. 2010 Feb 1;78(2):365-80. [PMID: 19722269] 

Functional Description

 Catalyzes the oxidation of either pyridoxine 5'- phosphate (PNP) or pyridoxamine 5'-phosphate (PMP) into pyridoxal 5'-phosphate (PLP). 

Sequence Annotation

 NP_BIND 88 89 FMN.
 NP_BIND 153 154 FMN.
 REGION 205 207 Substrate binding (By similarity).
 BINDING 73 73 FMN.
 BINDING 76 76 FMN; via amide nitrogen.
 BINDING 78 78 Substrate (By similarity).
 BINDING 96 96 FMN.
 BINDING 136 136 Substrate (By similarity).
 BINDING 140 140 Substrate (By similarity).
 BINDING 144 144 Substrate (By similarity).
 CROSSLNK 29 29 Glycyl lysine isopeptide (Lys-Gly)  

Keyword

 3D-structure; Complete proteome; Flavoprotein; FMN; Isopeptide bond; Oxidoreductase; Pyridoxine biosynthesis; Reference proteome; Ubl conjugation. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 228 AA 

Protein Sequence

Gene Ontology

 GO:0005758; C:mitochondrial intermembrane space; IDA:SGD.
 GO:0010181; F:FMN binding; IEA:InterPro.
 GO:0004733; F:pyridoxamine-phosphate oxidase activity; IMP:SGD.
 GO:0006631; P:fatty acid metabolic process; IMP:SGD.
 GO:0008615; P:pyridoxine biosynthetic process; IEA:UniProtKB-KW. 

Interpro

 IPR000659; Pyridox_Oxase.
 IPR019740; Pyridox_Oxase_CS.
 IPR011576; Pyridox_Oxase_FMN-bd.
 IPR019576; Pyridoxamine_oxidase_dimer_C.
 IPR012349; Split_barrel_FMN-bd. 

Pfam

 PF10590; PNPOx_C
 PF01243; Pyridox_oxidase 

SMART

  

PROSITE

 PS01064; PYRIDOX_OXIDASE 

PRINTS