CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-000927
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 TBC1 domain family member 4 
Protein Synonyms/Alias
 Akt substrate of 160 kDa; AS160 
Gene Name
 TBC1D4 
Gene Synonyms/Alias
 AS160; KIAA0603 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
68EIRRRSQKPEAGGCGubiquitination[1]
477GLYPPRAKLVIQRHLacetylation[2]
1255IRTLEQEKMAYQKTVubiquitination[1]
1267KTVEQLRKLLPADALubiquitination[1]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
 Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
 Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861] 
Functional Description
 May act as a GTPase-activating protein for RAB2A, RAB8A, RAB10 and RAB14. Isoform 2 promotes insulin-induced glucose transporter SLC2A4/GLUT4 translocation at the plasma membrane, thus increasing glucose uptake. 
Sequence Annotation
 DOMAIN 53 209 PID 1.
 DOMAIN 312 468 PID 2.
 DOMAIN 918 1112 Rab-GAP TBC.
 MOD_RES 1 1 N-acetylmethionine.
 MOD_RES 318 318 Phosphoserine; by PKB/AKT1 (By
 MOD_RES 341 341 Phosphoserine.
 MOD_RES 344 344 Phosphoserine.
 MOD_RES 477 477 N6-acetyllysine.
 MOD_RES 566 566 Phosphoserine.
 MOD_RES 568 568 Phosphothreonine.
 MOD_RES 570 570 Phosphoserine.
 MOD_RES 588 588 Phosphoserine; by PKB/AKT1.
 MOD_RES 591 591 Phosphoserine.
 MOD_RES 642 642 Phosphothreonine; by PKB/AKT1.
 MOD_RES 751 751 Phosphoserine; by PKB/AKT1 (By  
Keyword
 3D-structure; Acetylation; Alternative splicing; Complete proteome; Cytoplasm; GTPase activation; Phosphoprotein; Polymorphism; Reference proteome; Repeat. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1298 AA 
Protein Sequence
MEPPSCIQDE PFPHPLEPEP GVSAQPGPGK PSDKRFRLWY VGGSCLDHRT TLPMLPWLMA 60
EIRRRSQKPE AGGCGAPAAR EVILVLSAPF LRCVPAPGAG ASGGTSPSAT QPNPAVFIFE 120
HKAQHISRFI HNSHDLTYFA YLIKAQPDDP ESQMACHVFR ATDPSQVPDV ISSIRQLSKA 180
AMKEDAKPSK DNEDAFYNSQ KFEVLYCGKV TVTHKKAPSS LIDDCMEKFS LHEQQRLKIQ 240
GEQRGPDPGE DLADLEVVVP GSPGDCLPEE ADGTDTHLGL PAGASQPALT SSRVCFPERI 300
LEDSGFDEQQ EFRSRCSSVT GVQRRVHEGS QKSQPRRRHA SAPSHVQPSD SEKNRTMLFQ 360
VGRFEINLIS PDTKSVVLEK NFKDISSCSQ GIKHVDHFGF ICRESPEPGL SQYICYVFQC 420
ASESLVDEVM LTLKQAFSTA AALQSAKTQI KLCEACPMHS LHKLCERIEG LYPPRAKLVI 480
QRHLSSLTDN EQADIFERVQ KMKPVSDQEE NELVILHLRQ LCEAKQKTHV HIGEGPSTIS 540
NSTIPENATS SGRFKLDILK NKAKRSLTSS LENIFSRGAN RMRGRLGSVD SFERSNSLAS 600
EKDYSPGDSP PGTPPASPPS SAWQTFPEED SDSPQFRRRA HTFSHPPSST KRKLNLQDGR 660
AQGVRSPLLR QSSSEQCSNL SSVRRMYKES NSSSSLPSLH TSFSAPSFTA PSFLKSFYQN 720
SGRLSPQYEN EIRQDTASES SDGEGRKRTS STCSNESLSV GGTSVTPRRI SWRQRIFLRV 780
ASPMNKSPSA MQQQDGLDRN ELLPLSPLSP TMEEEPLVVF LSGEDDPEKI EERKKSKELR 840
SLWRKAIHQQ ILLLRMEKEN QKLEASRDEL QSRKVKLDYE EVGACQKEVL ITWDKKLLNC 900
RAKIRCDMED IHTLLKEGVP KSRRGEIWQF LALQYRLRHR LPNKQQPPDI SYKELLKQLT 960
AQQHAILVDL GRTFPTHPYF SVQLGPGQLS LFNLLKAYSL LDKEVGYCQG ISFVAGVLLL 1020
HMSEEQAFEM LKFLMYDLGF RKQYRPDMMS LQIQMYQLSR LLHDYHRDLY NHLEENEISP 1080
SLYAAPWFLT LFASQFSLGF VARVFDIIFL QGTEVIFKVA LSLLSSQETL IMECESFENI 1140
VEFLKNTLPD MNTSEMEKII TQVFEMDISK QLHAYEVEYH VLQDELQESS YSCEDSETLE 1200
KLERANSQLK RQNMDLLEKL QVAHTKIQAL ESNLENLLTR ETKMKSLIRT LEQEKMAYQK 1260
TVEQLRKLLP ADALVNCDLL LRDLNCNPNN KAKIGNKP 1298 
Gene Ontology
 GO:0030659; C:cytoplasmic vesicle membrane; TAS:Reactome.
 GO:0005097; F:Rab GTPase activator activity; IEA:InterPro.
 GO:0016044; P:cellular membrane organization; TAS:Reactome.
 GO:0032869; P:cellular response to insulin stimulus; IMP:UniProtKB.
 GO:0032851; P:positive regulation of Rab GTPase activity; IEA:GOC.
 GO:0016192; P:vesicle-mediated transport; IMP:UniProtKB. 
Interpro
 IPR021785; DUF3350.
 IPR011993; PH_like_dom.
 IPR006020; PTyr_interaction_dom.
 IPR000195; Rab-GTPase-TBC_dom. 
Pfam
 PF11830; DUF3350
 PF00640; PID
 PF00566; RabGAP-TBC 
SMART
 SM00462; PTB
 SM00164; TBC 
PROSITE
 PS01179; PID
 PS50086; TBC_RABGAP 
PRINTS