CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-019733
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Plakophilin-4 
Protein Synonyms/Alias
 p0071 
Gene Name
 PKP4 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
518PSIDSIQKDPREFAWubiquitination[1, 2, 3, 4, 5]
573VCRLGGIKHLVDLLDubiquitination[1, 4]
611DENKIAMKNVGGIPAubiquitination[6, 7]
625ALLRLLRKSIDAEVRacetylation[8]
683FDDDHKIKFQTSLVLubiquitination[1, 4, 9]
911RNKELIGKYAMRDLVubiquitination[1]
982RSSLKVVKAAAQVLNmethylation[10]
1175YVDFYSTKRPSYRAEubiquitination[1, 4]
Reference
 [1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [3] Proteome-wide identification of ubiquitylation sites by conjugation of engineered lysine-less ubiquitin.
 Oshikawa K, Matsumoto M, Oyamada K, Nakayama KI.
 J Proteome Res. 2012 Feb 3;11(2):796-807. [PMID: 22053931]
 [4] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
 [5] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [6] Tryptic digestion of ubiquitin standards reveals an improved strategy for identifying ubiquitinated proteins by mass spectrometry.
 Denis NJ, Vasilescu J, Lambert JP, Smith JC, Figeys D.
 Proteomics. 2007 Mar;7(6):868-74. [PMID: 17370265]
 [7] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [8] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
 Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
 Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861]
 [9] Mass spectrometric analysis of lysine ubiquitylation reveals promiscuity at site level.
 Danielsen JM, Sylvestersen KB, Bekker-Jensen S, Szklarczyk D, Poulsen JW, Horn H, Jensen LJ, Mailand N, Nielsen ML.
 Mol Cell Proteomics. 2011 Mar;10(3):M110.003590. [PMID: 21139048]
 [10] Large-scale global identification of protein lysine methylation in vivo.
 Cao XJ, Arnaudo AM, Garcia BA.
 Epigenetics. 2013 May 1;8(5):477-85. [PMID: 23644510] 
Functional Description
 Plays a role as a regulator of Rho activity during cytokinesis. May play a role in junctional plaques. 
Sequence Annotation
 REPEAT 415 455 ARM 1.
 REPEAT 518 557 ARM 2.
 REPEAT 560 599 ARM 3.
 REPEAT 604 644 ARM 4.
 REPEAT 660 702 ARM 5.
 REPEAT 706 751 ARM 6.
 REPEAT 815 855 ARM 7.
 REPEAT 862 901 ARM 8.
 REPEAT 950 993 ARM 9.
 MOD_RES 132 132 Phosphoserine (By similarity).
 MOD_RES 221 221 Phosphoserine.
 MOD_RES 231 231 Phosphoserine.
 MOD_RES 233 233 Phosphoserine (By similarity).
 MOD_RES 273 273 Phosphoserine.
 MOD_RES 275 275 Phosphotyrosine (By similarity).
 MOD_RES 281 281 Phosphoserine.
 MOD_RES 290 290 Phosphoserine (By similarity).
 MOD_RES 314 314 Phosphoserine.
 MOD_RES 327 327 Phosphoserine.
 MOD_RES 336 336 Phosphoserine (By similarity).
 MOD_RES 337 337 Phosphoserine (By similarity).
 MOD_RES 372 372 Phosphotyrosine (By similarity).
 MOD_RES 403 403 Phosphoserine.
 MOD_RES 406 406 Phosphoserine.
 MOD_RES 415 415 Phosphotyrosine.
 MOD_RES 422 422 Phosphoserine (By similarity).
 MOD_RES 447 447 Phosphoserine (By similarity).
 MOD_RES 478 478 Phosphotyrosine (By similarity).
 MOD_RES 510 510 Phosphoserine (By similarity).
 MOD_RES 512 512 Phosphoserine (By similarity).
 MOD_RES 515 515 Phosphoserine (By similarity).
 MOD_RES 776 776 Phosphoserine.
 MOD_RES 1048 1048 Phosphoserine (By similarity).
 MOD_RES 1049 1049 Phosphoserine (By similarity).
 MOD_RES 1135 1135 Phosphoserine (By similarity).
 MOD_RES 1138 1138 Phosphoserine (By similarity).
 CROSSLNK 611 611 Glycyl lysine isopeptide (Lys-Gly)  
Keyword
 Alternative splicing; Cell adhesion; Cell junction; Cell membrane; Coiled coil; Complete proteome; Cytoplasm; Cytoskeleton; Isopeptide bond; Membrane; Phosphoprotein; Reference proteome; Repeat; Ubl conjugation. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1192 AA 
Protein Sequence
MPAPEQASLV EEGQPQTRQE AASTGPGMEP ETTATTILAS VKEQELQFQR LTRELEVERQ 60
IVASQLERCR LGAESPSIAS TSSTEKSFPW RSTDVPNTGV SKPRVSDAVQ PNNYLIRTEP 120
EQGTLYSPEQ TSLHESEGSL GNSRSSTQMN SYSDSGYQEA GSFHNSQNVS KADNRQQHSF 180
IGSTNNHVVR NSRAEGQTLV QPSVANRAMR RVSSVPSRAQ SPSYVISTGV SPSRGSLRTS 240
LGSGFGSPSV TDPRPLNPSA YSSTTLPAAR AASPYSQRPA SPTAIRRIGS VTSRQTSNPN 300
GPTPQYQTTA RVGSPLTLTD AQTRVASPSQ GQVGSSSPKR SGMTAVPQHL GPSLQRTVHD 360
MEQFGQQQYD IYERMVPPRP DSLTGLRSSY ASQHSQLGQD LRSAVSPDLH ITPIYEGRTY 420
YSPVYRSPNH GTVELQGSQT ALYRTGSVGI GNLQRTSSQR STLTYQRNNY ALNTTATYAE 480
PYRPIQYRVQ ECNYNRLQHA VPADDGTTRS PSIDSIQKDP REFAWRDPEL PEVIHMLQHQ 540
FPSVQANAAA YLQHLCFGDN KVKMEVCRLG GIKHLVDLLD HRVLEVQKNA CGALRNLVFG 600
KSTDENKIAM KNVGGIPALL RLLRKSIDAE VRELVTGVLW NLSSCDAVKM TIIRDALSTL 660
TNTVIVPHSG WNNSSFDDDH KIKFQTSLVL RNTTGCLRNL SSAGEEARKQ MRSCEGLVDS 720
LLYVIHTCVN TSDYDSKTVE NCVCTLRNLS YRLELEVPQA RLLGLNELDD LLGKESPSKD 780
SEPSCWGKKK KKKKRTPQED QWDGVGPIPG LSKSPKGVEM LWHPSVVKPY LTLLAESSNP 840
ATLEGSAGSL QNLSAGNWKF AAYIRAAVRK EKGLPILVEL LRMDNDRVVS SVATALRNMA 900
LDVRNKELIG KYAMRDLVNR LPGGNGPSVL SDETMAAICC ALHEVTSKNM ENAKALADSG 960
GIEKLVNITK GRGDRSSLKV VKAAAQVLNT LWQYRDLRSI YKKDGWNQNH FITPVSTLER 1020
DRFKSHPSLS TTNQQMSPII QSVGSTSSSP ALLGIRDPRS EYDRTQPPMQ YYNSQGDATH 1080
KGLYPGSSKP SPIYISSYSS PAREQNRRLQ HQQLYYSQDD SNRKNFDAYR LYLQSPHSYE 1140
DPYFDDRVHF PASTDYSTQY GLKSTTNYVD FYSTKRPSYR AEQYPGSPDS WV 1192 
Gene Ontology
 GO:0030057; C:desmosome; IDA:UniProtKB.
 GO:0009898; C:internal side of plasma membrane; IDA:UniProtKB.
 GO:0030496; C:midbody; IDA:UniProtKB.
 GO:0072686; C:mitotic spindle; IDA:UniProtKB.
 GO:0048471; C:perinuclear region of cytoplasm; IDA:UniProtKB.
 GO:0051233; C:spindle midzone; IDA:UniProtKB.
 GO:0000922; C:spindle pole; IDA:UniProtKB.
 GO:0007155; P:cell adhesion; IEA:UniProtKB-KW.
 GO:0007043; P:cell-cell junction assembly; ISS:UniProtKB.
 GO:0007267; P:cell-cell signaling; NAS:UniProtKB.
 GO:0032467; P:positive regulation of cytokinesis; IMP:UniProtKB.
 GO:0010628; P:positive regulation of gene expression; ISS:UniProtKB.
 GO:0032321; P:positive regulation of Rho GTPase activity; IDA:UniProtKB.
 GO:0030155; P:regulation of cell adhesion; NAS:UniProtKB. 
Interpro
 IPR011989; ARM-like.
 IPR016024; ARM-type_fold.
 IPR000225; Armadillo. 
Pfam
 PF00514; Arm 
SMART
 SM00185; ARM 
PROSITE
 PS50176; ARM_REPEAT 
PRINTS