UniProt Accession

 VPS24_YEAST; P36095; D6VXP5 

Genbank Protein ID

 X71621; Z28041; AY557901; BK006944 

Genbank Nucleotide ID

 CAA50631.1; CAA81876.1; AAS56227.1; DAA09115.1 

Protein Name

 Vacuolar protein-sorting-associated protein 24 

Protein Synonyms/Alias

 DOA4-independent degradation protein 3; ESCRT-III complex subunit VPS24 

Gene Name

 VPS24 

Gene Synonyms/Alias

 DID3; YKL041W; YKL254 

Created Date

 July 27, 2013 

Organism

 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 

NCBI Taxa ID

 559292 

Lysine Modification

Position	Peptide	Type	References
203	EQEIPDEKVDEEADR	ubiquitination	[1, 2]

Reference

 [1] Sites of ubiquitin attachment in Saccharomyces cerevisiae.
 Starita LM, Lo RS, Eng JK, von Haller PD, Fields S.
 Proteomics. 2012 Jan;12(2):236-40. [PMID: 22106047]
 [2] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301] 

Functional Description

 Class E VPS protein implicated in concentration and sorting of cargo proteins of the multivesicular body (MVB) for incorporation into intralumenal vesicles. The lumenal sequestrated membrane proteins will be targeted into the vacuole after fusion of the endosome with the vacuole. Acts a component of the ESCRT- III complex, which appears to be critical for late steps in MVB sorting, such as membrane invagination and final cargo sorting and recruitment oflate-acting components of the sorting machinery. The MVB pathway requires the sequential function of ESCRT-O, -I,-II and -III complex assemblies. The DID4/VPS2-VPS24 subcomplex is required for the VPS4-dependent dissociation of ESCRT-III. 

Sequence Annotation

  

Keyword

 Complete proteome; Endosome; Membrane; Protein transport; Reference proteome; Transport. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 224 AA 

Protein Sequence

Gene Ontology

 GO:0012505; C:endomembrane system; IEA:UniProtKB-SubCell.
 GO:0000815; C:ESCRT III complex; IDA:SGD.
 GO:0070676; P:intralumenal vesicle formation; IMP:SGD.
 GO:0045324; P:late endosome to vacuole transport; IMP:SGD.
 GO:0015031; P:protein transport; IEA:UniProtKB-KW.
 GO:0043162; P:ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway; IC:SGD. 

Interpro

 IPR005024; Snf7. 

Pfam

 PF03357; Snf7 

SMART

  

PROSITE

  

PRINTS