CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-042460
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
  
Protein Name
 Mediator of RNA polymerase II transcription subunit 12 
Protein Synonyms/Alias
  
Gene Name
 MED12 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
43WVGERCLKSLCEDSNubiquitination[1, 2, 3, 4]
159MPSSSKTKPVLSSLEubiquitination[3, 4]
179LVAPLIAKLPTSVQGubiquitination[3]
190SVQGHVLKAAGEELEubiquitination[3, 4]
198AAGEELEKGQHLGSSubiquitination[3, 4]
363RAYMNLAKKLQKELGubiquitination[3]
364AYMNLAKKLQKELGEubiquitination[3]
367NLAKKLQKELGERQSubiquitination[3, 4]
379RQSDSLEKVRQLLPLubiquitination[3]
388RQLLPLPKQTRDVITubiquitination[3, 4]
406QGSLIDTKGNKIAGFubiquitination[2, 3, 4]
409LIDTKGNKIAGFDSIubiquitination[3, 4]
418AGFDSIFKKEGLQVSubiquitination[2, 3, 5]
419GFDSIFKKEGLQVSTubiquitination[3, 4]
427EGLQVSTKQKISPWDubiquitination[3]
429LQVSTKQKISPWDLFubiquitination[3]
543KRSQPATKTEDYGMGubiquitination[3, 4]
Reference
 [1] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [2] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [3] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [4] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [5] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473
Functional Description
  
Sequence Annotation
  
Keyword
 Complete proteome; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 925 AA 
Protein Sequence
GGSGGRRQGG RNISVETASL DVYAKYVLRS ICQQEWVGER CLKSLCEDSN DLQDPVLSSA 60
QAQRLMQLIC YPHRLLDNED GENPQRQRIK RILQNLDQWT MRQSSLELQL MIKQTPNNEM 120
NSLLENIAKA TIEVFQQSAE TGSSSGSTAS NMPSSSKTKP VLSSLERSGV WLVAPLIAKL 180
PTSVQGHVLK AAGEELEKGQ HLGSSSRKER DRQKQKSMSL LSQQPFLSLV LTCLKGQDEQ 240
REGLLTSLYS QVHQIVNNWR DDQYLDDCKP KQLMHEALKL RLNLVGGMFD TVQRSTQQTT 300
EWAMLLLEII ISGTVDMQSN NELFTTVLDM LSVLINGTLA ADMSSISQGS MEENKRAYMN 360
LAKKLQKELG ERQSDSLEKV RQLLPLPKQT RDVITCEPQG SLIDTKGNKI AGFDSIFKKE 420
GLQVSTKQKI SPWDLFEGLK PSAPLSWGWF GTVRVDRRVA RGEEQQRLLL YHTHLRPRPR 480
AYYLEPLPLP PEDEEPPAPT LLEPEKKAPE PPKTDKPGAA PPSTEERKKK STKGKKRSQP 540
ATKTEDYGMG PGRSGPYGVT VPPDLLHHPN PGSITHLNYR QGSIGLYTQN QPLPAGGPRV 600
DPYRPVRLPM QKLPTRPTYP GVLPTTMTGV MGLEPSSYKT SVYRQQQPAV PQGQRLRQQL 660
QAKIQSQGML GQSSVHQMTP SSSYGLQTSQ GYTPYVSHVG LQQHTGPAGT MVPPSYSSQP 720
YQSTHPSTNP TLVDPTRHLQ QRPSGYVHQQ APTYGHGLTS TQRFSHQTLQ QTPMISTMTP 780
MSAQGVQAGV RSTAILPEQQ QQQQQQQQQQ QQQQQQQQQQ QQQQYHIRQQ QQQQILRQQQ 840
QQQQQQQQQQ QQQQQQQQQQ QQQHQQQQQQ QAAPPQPQPQ SQPQFQRQGL QQTQQQQQTA 900
ALVRQLQQQL SNTQPQPSTN IFGRY 925 
Gene Ontology
 GO:0016592; C:mediator complex; IEA:InterPro. 
Interpro
 IPR021989; Mediator_Med12_catenin-bd. 
Pfam
 PF12144; Med12-PQL 
SMART
  
PROSITE
  
PRINTS