CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-030862
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 M-phase inducer phosphatase 2 
Protein Synonyms/Alias
 cDNA FLJ53333, highly similar to M-phase inducer phosphatase 2 (EC 3.1.3.48) 
Gene Name
 CDC25B 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
7*MHSETPKSQVGTLLubiquitination[1]
113QAPDGRRKSEAGSGAubiquitination[1]
140FVFKMPWKPTHPSSTubiquitination[1, 2]
178MCLSPDRKMEVEELSubiquitination[1, 2, 3, 4]
235LISAPLVKTLEKEEEubiquitination[5]
239PLVKTLEKEEEKDLVubiquitination[1, 2, 3, 4]
243TLEKEEEKDLVMYSKubiquitination[3]
250KDLVMYSKCQRLFRSubiquitination[6]
270SVIRPILKRLERPQDubiquitination[1]
303QQEAEEPKARVLRSKubiquitination[1]
310KARVLRSKSLCHDEIubiquitination[1]
334ELIGDYSKAFLLQTVubiquitination[1, 2, 3, 4, 5, 6]
344LLQTVDGKHQDLKYIubiquitination[1, 3, 4]
349DGKHQDLKYISPETMubiquitination[1, 2, 3]
370KFSNIVDKFVIVDCRubiquitination[1]
388EYEGGHIKTAVNLPLubiquitination[1, 3, 4]
405DAESFLLKSPIAPCSubiquitination[1]
415IAPCSLDKRVILIFHubiquitination[1]
459YPEMYILKGGYKEFFubiquitination[2, 3, 6]
463YILKGGYKEFFPQHPubiquitination[1]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [3] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [4] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [5] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [6] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094
Functional Description
  
Sequence Annotation
  
Keyword
 Complete proteome; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 516 AA 
Protein Sequence
MHSETPKSQV GTLLFRSRSR LTHLSLSRRA SESSLSSESS ESSDAGLCMD SPSPMDPHMA 60
EQTFEQAIQA ASRIIRNEQF AIRRFQSMPV RLLGHSPVLR NITNSQAPDG RRKSEAGSGA 120
ASSSGEDKEN DGFVFKMPWK PTHPSSTHAL AEWASRREAF AQRPSSAPDL MCLSPDRKME 180
VEELSPLALG RFSLTPAEGD TEEDDGFVDI LESDLKDDDA VPPGMESLIS APLVKTLEKE 240
EEKDLVMYSK CQRLFRSPSM PCSVIRPILK RLERPQDRDT PVQNKRRRSV TPPEEQQEAE 300
EPKARVLRSK SLCHDEIENL LDSDHRELIG DYSKAFLLQT VDGKHQDLKY ISPETMVALL 360
TGKFSNIVDK FVIVDCRYPY EYEGGHIKTA VNLPLERDAE SFLLKSPIAP CSLDKRVILI 420
FHCEFSSERG PRMCRFIRER DRAVNDYPSL YYPEMYILKG GYKEFFPQHP NFCEPQDYRP 480
MNHEAFKDEL KTFRLKTRSW AGERSRRELC SRLQDQ 516 
Gene Ontology
 GO:0005622; C:intracellular; IEA:InterPro.
 GO:0004725; F:protein tyrosine phosphatase activity; IEA:InterPro.
 GO:0035335; P:peptidyl-tyrosine dephosphorylation; IEA:GOC. 
Interpro
 IPR000751; MPI_Phosphatase.
 IPR001763; Rhodanese-like_dom. 
Pfam
 PF06617; M-inducer_phosp
 PF00581; Rhodanese 
SMART
 SM00450; RHOD 
PROSITE
 PS50206; RHODANESE_3 
PRINTS
 PR00716; MPIPHPHTASE.