UniProt Accession

 MCM10_YEAST; P32354; D6VVD7 

Genbank Protein ID

 Z38059; M83540; BK006942 

Genbank Nucleotide ID

 CAA86128.1; AAA34574.1; DAA08403.1 

Protein Name

 Minichromosome maintenance protein 10 

Protein Synonyms/Alias

 Protein DNA43 

Gene Name

 MCM10 

Gene Synonyms/Alias

 DNA43; YIL150C 

Created Date

 July 27, 2013 

Organism

 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 

NCBI Taxa ID

 559292 

Lysine Modification

Position	Peptide	Type	References
491	GFDPTHGKISQVLKS	acetylation	[1]

Reference

 [1] Proteome-wide analysis of lysine acetylation suggests its broad regulatory scope in Saccharomyces cerevisiae.
 Henriksen P, Wagner SA, Weinert BT, Sharma S, Bacinskaja G, Rehman M, Juffer AH, Walther TC, Lisby M, Choudhary C.
 Mol Cell Proteomics. 2012 Nov;11(11):1510-22. [PMID: 22865919] 

Functional Description

 Required for DNA synthesis. Required for entry into or completion of S phase. Involved in DNA replication and seems to participate in the activation of the pre-replication complex (pre- RC) and in transcription elongation. May play a role as a key coordinator in assembling the replication fork. Proposed to function at replication origins following the binding of the MCM2- 7 complex prior to the recruitment of CDC45. Probably is required to stimulate phosphorylation of the MCM2-7 complex by the CDC7- DBF4 kinase complex. May recruit the DNA polymerase alpha:primase complex to replication origins and is required to maintain it on chromatin independently of CDC45. May also play a role in transcriptional silencing. 

Sequence Annotation

 REGION 309 335 Zinc finger-like.
 REGION 435 512 Sufficient for nuclear localization.
 REGION 453 553 Sufficient for nuclear localization.
 MOTIF 435 451 Bipartite nuclear localization signal (By
 MOTIF 512 527 Bipartite nuclear localization signal (By
 MOD_RES 17 17 Phosphothreonine.
 MOD_RES 18 18 Phosphoserine.
 MOD_RES 453 453 Phosphoserine.
 MOD_RES 454 454 Phosphoserine.  

Keyword

 Cell cycle; Complete proteome; DNA replication; Metal-binding; Nucleus; Phosphoprotein; Reference proteome; Ubl conjugation; Zinc; Zinc-finger. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 571 AA 

Protein Sequence

Gene Ontology

 GO:0031298; C:replication fork protection complex; IDA:SGD.
 GO:0003688; F:DNA replication origin binding; IDA:SGD.
 GO:0003690; F:double-stranded DNA binding; IDA:SGD.
 GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
 GO:0003697; F:single-stranded DNA binding; IDA:SGD.
 GO:0007049; P:cell cycle; IEA:UniProtKB-KW.
 GO:0030466; P:chromatin silencing at silent mating-type cassette; IMP:SGD.
 GO:0006348; P:chromatin silencing at telomere; IMP:SGD.
 GO:0006270; P:DNA replication initiation; IMP:SGD.
 GO:0006271; P:DNA strand elongation involved in DNA replication; IMP:SGD.
 GO:0000727; P:double-strand break repair via break-induced replication; IMP:SGD. 

Interpro

 IPR012340; NA-bd_OB-fold.
 IPR015408; Znf_Mcm10/DnaG. 

Pfam

 PF09329; zf-primase 

SMART

  

PROSITE

  

PRINTS