CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-026397
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Abnormal spindle-like microcephaly-associated protein 
Protein Synonyms/Alias
 Asp (Abnormal spindle)-like, microcephaly associated (Drosophila), isoform CRA_d 
Gene Name
 ASPM 
Gene Synonyms/Alias
 hCG_2039667 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
27CRLFTSEKMVKAIKKubiquitination[1]
54RKDRHLWKDVGERQKubiquitination[1, 2]
165HDPCLFCKDAEFKASubiquitination[1, 2]
173DAEFKASKEILLAFSubiquitination[1]
244QNWDLSKKLRIPAISubiquitination[1]
267DIVLQVLKSRGIELSubiquitination[1, 2]
331KHTKSIKKTISLLSCubiquitination[1]
347SDDLINKKKGKRDSGubiquitination[1]
465ELLENEKKNFHLVRSubiquitination[1]
584RRVLAQRKLLMLKKEubiquitination[1]
625CKVKAACKIQAWYRCubiquitination[1]
637YRCWRAHKEYLAILKubiquitination[2]
656IQGCFYTKLERTRFLubiquitination[2, 3]
683WRAILPAKIAHEHFLubiquitination[1, 3]
693HEHFLMIKRHRAACLubiquitination[1, 2]
718RQVFLRQKSAALIIQubiquitination[1]
726SAALIIQKYIRAREAubiquitination[1, 2, 4]
800YKLYLAVKNANKQVNubiquitination[1]
804LAVKNANKQVNSVICubiquitination[1, 2]
828QEKRFIQKYHSIKKIubiquitination[1]
834QKYHSIKKIEHEGQEubiquitination[1, 2]
855RAASVIQKAVRHFLLubiquitination[1]
1029PGNKVADKGGSIFTKubiquitination[1, 2]
1069DRIYSLYKLTAHKHKubiquitination[1, 4]
1087ERILYKQKKNSSISIubiquitination[1]
1088RILYKQKKNSSISIPubiquitination[1]
1111TRIVSRLKPDWVLRRubiquitination[1, 2]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [3] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [4] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473
Functional Description
  
Sequence Annotation
  
Keyword
 Complete proteome; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1142 AA 
Protein Sequence
MSLRAYTARC RLNRLRRAAC RLFTSEKMVK AIKKLEIEIE ARRLIVRKDR HLWKDVGERQ 60
KVLNWLLSYN PLWLRIGLET TYGELISLED NSDVTGLAMF ILNRLLWNPD IAAEYRHPTV 120
PHLYRDGHEE ALSKFTLKKL LLLVCFLDYA KISRLIDHDP CLFCKDAEFK ASKEILLAFS 180
RDFLSGEGDL SRHLGLLGLP VNHVQTPFDE FDFAVTNLAV DLQCGVRLVR TMELLTQNWD 240
LSKKLRIPAI SRLQKMHNVD IVLQVLKSRG IELSDEHGNT ILSKDIVDRH REKTLRLLWK 300
IAFAFQVDIS LNLDQLKEEI AFLKHTKSIK KTISLLSCHS DDLINKKKGK RDSGSFEQYS 360
ENIKLLMDWV NAVCAFYNKK VENFTVSFSD GRVLCYLIHH YHPCYVPFDA ICQRTTQTVE 420
CTQTGSVVLN SSSESDDSSL DMSLKAFDHE NTSELYKELL ENEKKNFHLV RSAVRDLGGI 480
PAMINHSDMS NTIPDEKVVI TYLSFLCARL LDLRKEIRAA RLIQTTWRKY KLKTDLKRHQ 540
EREKAARIIQ LAVINFLAKQ RLRKRVNAAL VIQKYWRRVL AQRKLLMLKK EKLEKVQNKA 600
ASLIQAMWRR YRAKKYLCKV KAACKIQAWY RCWRAHKEYL AILKAVKIIQ GCFYTKLERT 660
RFLNVRASAI IIQRKWRAIL PAKIAHEHFL MIKRHRAACL IQAHYRGYKG RQVFLRQKSA 720
ALIIQKYIRA REAGKHERIK YIEFKKSTVI LQALVRGWLV RKRFLEQRAK IRLLHFTAAA 780
YYHLNAVRIQ RAYKLYLAVK NANKQVNSVI CIQRWFRARL QEKRFIQKYH SIKKIEHEGQ 840
ECLSQRNRAA SVIQKAVRHF LLRKKQEKFT SGIIKIQALW RGYSWRKKND CTKIKAIRLS 900
LQVVNREIRE ENKLYKRTAL ALHYLLTYKH LSAILEALKH LEVVTRLSPL CCENMAQSGA 960
ISKIFVLIRS CNRSIPCMEV IRYAVQVLLN VSKYEKTTSA VYDVENCIDI LLELLQIYRE 1020
KPGNKVADKG GSIFTKTCCL LAILLKTTNR ASDVRSRSKV VDRIYSLYKL TAHKHKMNTE 1080
RILYKQKKNS SISIPFIPET PVRTRIVSRL KPDWVLRRDN MEEITNPLQA IQMVMDTLGI 1140
PY 1142 
Gene Ontology
  
Interpro
 IPR011989; ARM-like.
 IPR022613; CAMSAP_CH.
 IPR001715; CH-domain.
 IPR000048; IQ_motif_EF-hand-BS.
 IPR027417; P-loop_NTPase. 
Pfam
 PF11971; CAMSAP_CH
 PF00612; IQ 
SMART
 SM00033; CH
 SM00015; IQ 
PROSITE
 PS50021; CH
 PS50096; IQ 
PRINTS