CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-039479
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
  
Protein Name
 tRNA (uracil-5-)-methyltransferase homolog A 
Protein Synonyms/Alias
  
Gene Name
 TRMT2A 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
104GLQPHKTKLFGQPPCubiquitination[1]
135VLHGALWKGRPLSVRubiquitination[1]
147SVRLARPKADPMARRubiquitination[1]
187YAEQLERKQLECEQVubiquitination[1, 2]
197ECEQVLQKLAKEIGSubiquitination[1]
200QVLQKLAKEIGSTNRubiquitination[1]
242QQTEYRNKCEFLVGVubiquitination[1]
266GCRLGKYKGGTCAVAubiquitination[1, 2]
290EATKQVVKAFQEFIRubiquitination[3, 4]
314ETYTGHWKQLTVRTSubiquitination[1, 3, 4]
346PEELAELKTSLAQHFubiquitination[1, 2, 3, 4]
375FVEEGQRKTPSQEGLubiquitination[1, 2]
577RAPSNRVKGIPFRPVubiquitination[1, 2]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [3] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [4] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789] 
Functional Description
  
Sequence Annotation
  
Keyword
 Complete proteome; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 643 AA 
Protein Sequence
MSENLDNEGP KPMESCGQES SSALSCPTVS VPPAAPAALE EVEKEGAGAA TGPGPQPGLY 60
SYIRDDLFTS EIFKLELQNV PRHASFSDVR RFLGRFGLQP HKTKLFGQPP CAFVTFRSAA 120
ERDKALRVLH GALWKGRPLS VRLARPKADP MARRRRQEGE SEPPVTRVAD VVTPLWTVPY 180
AEQLERKQLE CEQVLQKLAK EIGSTNRALL PWLLEQRHKH NKACCPLEGV RPSPQQTEYR 240
NKCEFLVGVG VDGEDNTVGC RLGKYKGGTC AVAAPFDTVH IPEATKQVVK AFQEFIRSTP 300
YSAYDPETYT GHWKQLTVRT SRRHQAMAIA YFHPQKLSPE ELAELKTSLA QHFTAGPGRA 360
SGVTCLYFVE EGQRKTPSQE GLPLEHVAGD RCIHEDLLGL TFRISPHAFF QVNTPAAEVL 420
YTVIQDWAQL DAGSMVLDVC CGTGTIGLAL ARGPMYSPPW VGRHHAFLFQ KVKRVIGVEL 480
CPEAVEDARV NAQDNELSNV EFHCGRAEDL VPTLVSRLAS QHLVAILDPP RAGLHSKVIL 540
AIRRAKNLRR LLYVSCNPRA AMGNFVDLCR APSNRVKGIP FRPVKAVAVD LFPQTPHCEM 600
LILFERVEHP NGTGVLGPHS PPAQPTPGPP DNTLQETGTF PSS 643 
Gene Ontology
 GO:0003676; F:nucleic acid binding; IEA:InterPro.
 GO:0000166; F:nucleotide binding; IEA:InterPro. 
Interpro
 IPR025714; Methyltranfer_dom.
 IPR012677; Nucleotide-bd_a/b_plait.
 IPR000504; RRM_dom. 
Pfam
 PF13847; Methyltransf_31 
SMART
 SM00360; RRM 
PROSITE
 PS50102; RRM 
PRINTS