UniProt Accession

 APN1_YEAST; P22936; D6VXH4 

Genbank Protein ID

 M33667; S93804; Z28114; BK006944 

Genbank Nucleotide ID

 AAA34429.1; AAB22003.1; CAA81954.1; DAA09044.1 

Protein Name

 DNA-(apurinic or apyrimidinic site) lyase 1 

Protein Synonyms/Alias

 Apurinic-apyrimidinic endonuclease 1; AP endonuclease 1 

Gene Name

 APN1 

Gene Synonyms/Alias

 YKL114C; YKL513 

Created Date

 July 27, 2013 

Organism

 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 

NCBI Taxa ID

 559292 

Lysine Modification

Position	Peptide	Type	References
316	EKNDTLQKLGAKSRK	acetylation	[1]
328	SRKEQLDKFEVKQKK	acetylation	[1]

Reference

 [1] Proteome-wide analysis of lysine acetylation suggests its broad regulatory scope in Saccharomyces cerevisiae.
 Henriksen P, Wagner SA, Weinert BT, Sharma S, Bacinskaja G, Rehman M, Juffer AH, Walther TC, Lisby M, Choudhary C.
 Mol Cell Proteomics. 2012 Nov;11(11):1510-22. [PMID: 22865919] 

Functional Description

 DNA repair enzyme that cleaves apurinic/apyrimidinic (AP) sites and removes 3'-blocking groups present at single strand breaks of damaged DNA. APN1 accounts for > 97% of both apurinic/ apyrimidinic (AP) lyase and DNA 3'-repair diesterase activities. 

Sequence Annotation

 METAL 83 83 Zinc 1 (By similarity).
 METAL 123 123 Zinc 1 (By similarity).
 METAL 158 158 Zinc 1 (By similarity).
 METAL 158 158 Zinc 2 (By similarity).
 METAL 192 192 Zinc 2 (By similarity).
 METAL 195 195 Zinc 3 (By similarity).
 METAL 229 229 Zinc 2 (By similarity).
 METAL 242 242 Zinc 3 (By similarity).
 METAL 244 244 Zinc 3 (By similarity).
 METAL 274 274 Zinc 2 (By similarity).
 MOD_RES 356 356 Phosphoserine.  

Keyword

 Complete proteome; Direct protein sequencing; DNA damage; DNA repair; Lyase; Metal-binding; Nucleus; Phosphoprotein; Reference proteome; Zinc. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 367 AA 

Protein Sequence

Gene Ontology

 GO:0005739; C:mitochondrion; IDA:SGD.
 GO:0005634; C:nucleus; IDA:SGD.
 GO:0017005; F:3'-tyrosyl-DNA phosphodiesterase activity; IDA:SGD.
 GO:0003677; F:DNA binding; IEA:InterPro.
 GO:0003906; F:DNA-(apurinic or apyrimidinic site) lyase activity; IDA:SGD.
 GO:0008311; F:double-stranded DNA specific 3'-5' exodeoxyribonuclease activity; IDA:SGD.
 GO:0004519; F:endonuclease activity; IEA:InterPro.
 GO:0008270; F:zinc ion binding; IEA:InterPro.
 GO:0006284; P:base-excision repair; IDA:SGD. 

Interpro

 IPR018246; AP_endonuc_F2_Zn_BS.
 IPR001719; Endodeoxyribonuclease_IV.
 IPR013022; Xyl_isomerase-like_TIM-brl. 

Pfam

 PF01261; AP_endonuc_2 

SMART

 SM00518; AP2Ec 

PROSITE

 PS00729; AP_NUCLEASE_F2_1
 PS00730; AP_NUCLEASE_F2_2
 PS00731; AP_NUCLEASE_F2_3
 PS51432; AP_NUCLEASE_F2_4 

PRINTS