CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-005507
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Mitotic check point protein BUB2 
Protein Synonyms/Alias
 Cell cycle arrest protein BUB2 
Gene Name
 BUB2 
Gene Synonyms/Alias
 YMR055C; YM9796.08C 
Created Date
 July 27, 2013 
Organism
 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 
NCBI Taxa ID
 559292 
Lysine Modification
Position
Peptide
Type
References
37GLPISEDKQQQRTRCubiquitination[1]
Reference
 [1] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301] 
Functional Description
 Part of a checkpoint which monitors spindle integrity and prevents premature exit from mitosis. This cell-cycle arrest depends upon inhibition of the GTP-binding protein TEM1 by the BFA1/BUB2 complex. 
Sequence Annotation
 DOMAIN 37 235 Rab-GAP TBC.  
Keyword
 Cell cycle; Cell division; Complete proteome; Cytoplasm; Cytoskeleton; Mitosis; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 306 AA 
Protein Sequence
MTSIEDLISN PPLLLHSSLS QLRYLILSEG LPISEDKQQQ RTRCYVWTVL SQTSMEASTQ 60
RYLALLKLGP PSTTIYQKIK NDTSRTFQTD PNFRNRVSED ALIRCLSCFA WQTQQRRQKT 120
RFGRIPVSTY VQGMNVLLAP LLYSCPSEPM AYQLFTKLCY EMIPTYLTKN LNGAQNGAKL 180
LDISLRIIDP KLSKFLSDNL LTAEIYGMPS ILTLSSCNKP LDQVIKLWDF MFAYGFHMNI 240
LFVVAFLVKM RSKVFKSDSP VNLLRQFPDF DADEIIRLGV GFIAKIPAQI YDLLVDHLTD 300
PDIYIP 306 
Gene Ontology
 GO:0005737; C:cytoplasm; IEA:UniProtKB-KW.
 GO:0005816; C:spindle pole body; IDA:SGD.
 GO:0005096; F:GTPase activator activity; TAS:SGD.
 GO:0005097; F:Rab GTPase activator activity; IEA:InterPro.
 GO:0051301; P:cell division; IEA:UniProtKB-KW.
 GO:0007067; P:mitosis; IEA:UniProtKB-KW.
 GO:0031578; P:mitotic spindle orientation checkpoint; IGI:SGD.
 GO:0032851; P:positive regulation of Rab GTPase activity; IEA:GOC. 
Interpro
 IPR000195; Rab-GTPase-TBC_dom. 
Pfam
 PF00566; RabGAP-TBC 
SMART
 SM00164; TBC 
PROSITE
 PS50086; TBC_RABGAP 
PRINTS