CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-001570
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 RasGAP-activating-like protein 1 
Protein Synonyms/Alias
  
Gene Name
 RASAL1 
Gene Synonyms/Alias
 RASAL 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
174LETSTIKKTRFPHWDubiquitination[1]
349RSNSLASKSMEQFMKubiquitination[1]
399RTRRISFKGALSEEQubiquitination[1]
482APAILTPKLFDLRDQubiquitination[1]
504RSLLLLAKAVQSIGNubiquitination[2]
574VREGYLLKRKEEPAGubiquitination[1]
576EGYLLKRKEEPAGLAubiquitination[1]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983] 
Functional Description
 Probable inhibitory regulator of the Ras-cyclic AMP pathway. 
Sequence Annotation
 DOMAIN 1 88 C2 1.
 DOMAIN 120 216 C2 2.
 DOMAIN 301 511 Ras-GAP.
 DOMAIN 565 672 PH.
 ZN_FING 674 710 Btk-type.  
Keyword
 Alternative splicing; Complete proteome; GTPase activation; Metal-binding; Polymorphism; Reference proteome; Repeat; Zinc; Zinc-finger. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 804 AA 
Protein Sequence
MAKSSSLNVR VVEGRALPAK DVSGSSDPYC LVKVDDEVVA RTATVWRSLG PFWGEEYTVH 60
LPLDFHQLAF YVLDEDTVGH DDIIGKISLS REAITADPRG IDSWINLSRV DPDAEVQGEI 120
CLSVQMLEDG QGRCLRCHVL QARDLAPRDI SGTSDPFARV FWGSQSLETS TIKKTRFPHW 180
DEVLELREMP GAPSPLRVEL WDWDMVGKND FLGMVEFSPK TLQQKPPKGW FRLLPFPRAE 240
EDSGGNLGAL RVKVRLIEDR VLPSQCYQPL MELLMESVQG PAEEDTASPL ALLEELTLGD 300
CRQDLATKLV KLFLGRGLAG RFLDYLTRRE VARTMDPNTL FRSNSLASKS MEQFMKLVGM 360
PYLHEVLKPV ISRVFEEKKY MELDPCKMDL GRTRRISFKG ALSEEQMRET SLGLLTGYLG 420
PIVDAIVGSV GRCPPAMRLA FKQLHRRVEE RFPQAEHQDV KYLAISGFLF LRFFAPAILT 480
PKLFDLRDQH ADPQTSRSLL LLAKAVQSIG NLGQQLGQGK ELWMAPLHPF LLQCVSRVRD 540
FLDRLVDVDG DEAGVPARAL FPPSAIVREG YLLKRKEEPA GLATRFAFKK RYVWLSGETL 600
SFSKSPEWQM CHSIPVSHIR AVERVDEGAF QLPHVMQVVT QDGTGALHTT YLQCKNVNEL 660
NQWLSALRKA SAPNPNKLAA CHPGAFRSAR WTCCLQAERS AAGCSRTHSA VTLGDWSDPL 720
DPDAEAQTVY RQLLLGRDQL RLKLLEDSNM DTTLEADTGA CPEVLARQRA ATARLLEVLA 780
DLDRAHEEFQ QQERGKAALG PLGP 804 
Gene Ontology
 GO:0005737; C:cytoplasm; IBA:RefGenome.
 GO:0031235; C:intrinsic to internal side of plasma membrane; IBA:RefGenome.
 GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
 GO:0005543; F:phospholipid binding; TAS:ProtInc.
 GO:0005099; F:Ras GTPase activator activity; IBA:RefGenome.
 GO:0035556; P:intracellular signal transduction; IEA:InterPro.
 GO:0046580; P:negative regulation of Ras protein signal transduction; IBA:RefGenome.
 GO:0007165; P:signal transduction; TAS:ProtInc. 
Interpro
 IPR000008; C2_Ca-dep.
 IPR008973; C2_Ca/lipid-bd_dom_CaLB.
 IPR018029; C2_membr_targeting.
 IPR011993; PH_like_dom.
 IPR001849; Pleckstrin_homology.
 IPR001936; RasGAP.
 IPR023152; RasGAP_CS.
 IPR008936; Rho_GTPase_activation_prot.
 IPR001562; Znf_Btk_motif. 
Pfam
 PF00779; BTK
 PF00168; C2
 PF00169; PH
 PF00616; RasGAP 
SMART
 SM00107; BTK
 SM00239; C2
 SM00233; PH
 SM00323; RasGAP 
PROSITE
 PS50004; C2
 PS50003; PH_DOMAIN
 PS00509; RAS_GTPASE_ACTIV_1
 PS50018; RAS_GTPASE_ACTIV_2
 PS51113; ZF_BTK 
PRINTS