CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-006852
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 DNA replication licensing factor MCM7 
Protein Synonyms/Alias
 Cell division control protein 47; Minichromosome maintenance protein 7 
Gene Name
 MCM7 
Gene Synonyms/Alias
 CDC47; YBR202W; YBR1441 
Created Date
 July 27, 2013 
Organism
 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 
NCBI Taxa ID
 559292 
Lysine Modification
Position
Peptide
Type
References
724LRLVRVSKESLYQETubiquitination[1]
Reference
 [1] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301] 
Functional Description
 Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. Once loaded onto DNA, double hexamers can slide on dsDNA in the absence of ATPase activity. 
Sequence Annotation
 DOMAIN 410 617 MCM.
 NP_BIND 460 467 ATP (Potential).
 MOTIF 592 595 Arginine finger.
 MOD_RES 811 811 Phosphothreonine.
 MOD_RES 819 819 Phosphoserine.
 MOD_RES 838 838 Phosphoserine.  
Keyword
 ATP-binding; Cell cycle; Complete proteome; Cytoplasm; DNA replication; DNA-binding; Helicase; Hydrolase; Nucleotide-binding; Nucleus; Phosphoprotein; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 845 AA 
Protein Sequence
MSAALPSIQL PVDYNNLFNE ITDFLVTFKQ DTLSSDATRN ENEDENLDAE NIEQHLLEKG 60
PKYMAMLQKV ANRELNSVII DLDDILQYQN EKFLQGTQAD DLVSAIQQNA NHFTELFCRA 120
IDNNMPLPTK EIDYKDDVLD VILNQRRLRN ERMLSDRTNE IRSENLMDTT MDPPSSMNDA 180
LREVVEDETE LFPPNLTRRY FLYFKPLSQN CARRYRKKAI SSKPLSVRQI KGDFLGQLIT 240
VRGIITRVSD VKPAVEVIAY TCDQCGYEVF QEVNSRTFTP LSECTSEECS QNQTKGQLFM 300
STRASKFSAF QECKIQELSQ QVPVGHIPRS LNIHVNGTLV RSLSPGDIVD VTGIFLPAPY 360
TGFKALKAGL LTETYLEAQF VRQHKKKFAS FSLTSDVEER VMELITSGDV YNRLAKSIAP 420
EIYGNLDVKK ALLLLLVGGV DKRVGDGMKI RGDINVCLMG DPGVAKSQLL KAICKISPRG 480
VYTTGKGSSG VGLTAAVMKD PVTDEMILEG GALVLADNGI CCIDEFDKMD ESDRTAIHEV 540
MEQQTISISK AGINTTLNAR TSILAAANPL YGRYNPRLSP LDNINLPAAL LSRFDILFLM 600
LDIPSRDDDE KLAEHVTYVH MHNKQPDLDF TPVEPSKMRE YIAYAKTKRP VMSEAVNDYV 660
VQAYIRLRQD SKREMDSKFS FGQATPRTLL GIIRLSQALA KLRLADMVDI DDVEEALRLV 720
RVSKESLYQE TNKSKEDESP TTKIFTIIKK MLQETGKNTL SYENIVKTVR LRGFTMLQLS 780
NCIQEYSYLN VWHLINEGNT LKFVDDGTMD TDQEDSLVST PKLAPQTTAS ANVSAQDSDI 840
DLQDA 845 
Gene Ontology
 GO:0005737; C:cytoplasm; IDA:SGD.
 GO:0031261; C:DNA replication preinitiation complex; IDA:SGD.
 GO:0042555; C:MCM complex; IDA:SGD.
 GO:0005656; C:pre-replicative complex; IDA:SGD.
 GO:0031298; C:replication fork protection complex; IDA:SGD.
 GO:0005524; F:ATP binding; IDA:SGD.
 GO:0003682; F:chromatin binding; IDA:SGD.
 GO:0003688; F:DNA replication origin binding; IDA:SGD.
 GO:0009378; F:four-way junction helicase activity; IDA:SGD.
 GO:0043142; F:single-stranded DNA-dependent ATPase activity; IDA:SGD.
 GO:0003712; F:transcription cofactor activity; IDA:SGD.
 GO:0007049; P:cell cycle; IEA:UniProtKB-KW.
 GO:0030466; P:chromatin silencing at silent mating-type cassette; IMP:SGD.
 GO:0006348; P:chromatin silencing at telomere; IMP:SGD.
 GO:0006270; P:DNA replication initiation; IMP:SGD.
 GO:0006271; P:DNA strand elongation involved in DNA replication; IMP:SGD.
 GO:0006268; P:DNA unwinding involved in replication; IDA:SGD.
 GO:0000727; P:double-strand break repair via break-induced replication; IMP:SGD.
 GO:0006267; P:pre-replicative complex assembly; IDA:SGD.
 GO:0006357; P:regulation of transcription from RNA polymerase II promoter; IDA:SGD. 
Interpro
 IPR003593; AAA+_ATPase.
 IPR008050; MCM7.
 IPR018525; MCM_CS.
 IPR001208; MCM_DNA-dep_ATPase.
 IPR012340; NA-bd_OB-fold.
 IPR027417; P-loop_NTPase. 
Pfam
 PF00493; MCM 
SMART
 SM00382; AAA
 SM00350; MCM 
PROSITE
 PS00847; MCM_1
 PS50051; MCM_2 
PRINTS
 PR01657; MCMFAMILY.
 PR01663; MCMPROTEIN7.