CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-022421
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 ADP-ribosylation factor-binding protein GGA3 
Protein Synonyms/Alias
 Golgi-localized, gamma ear-containing, ARF-binding protein 3 
Gene Name
 GGA3 
Gene Synonyms/Alias
 KIAA0154 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
15SLESWLNKATNPSNRubiquitination[1, 2, 3]
53AVRLLAHKIQSPQEWubiquitination[2]
100LIKVVSPKYLGDRVSubiquitination[1, 2, 3, 4, 5]
139KDAYHMLKRQGIVQSubiquitination[4]
165PSPPPRPKNPVFDDEubiquitination[1, 2, 3]
174PVFDDEEKSKLLAKLubiquitination[2, 4]
176FDDEEKSKLLAKLLKubiquitination[2]
180EKSKLLAKLLKSKNPubiquitination[1, 2, 3, 4, 5]
183KLLAKLLKSKNPDDLubiquitination[2]
185LAKLLKSKNPDDLQEubiquitination[2, 4]
195DDLQEANKLIKSMVKubiquitination[1, 2, 3, 4]
198QEANKLIKSMVKEDEubiquitination[1, 2, 3]
202KLIKSMVKEDEARIQubiquitination[2, 4]
258LFDQCENKRRTLFKLubiquitination[2]
264NKRRTLFKLASETEDubiquitination[2, 4, 6]
Reference
 [1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [3] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [4] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [5] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [6] Proteome-wide identification of ubiquitylation sites by conjugation of engineered lysine-less ubiquitin.
 Oshikawa K, Matsumoto M, Oyamada K, Nakayama KI.
 J Proteome Res. 2012 Feb 3;11(2):796-807. [PMID: 22053931
Functional Description
 Plays a role in protein sorting and trafficking between the trans-Golgi network (TGN) and endosomes. Mediates the ARF- dependent recruitment of clathrin to the TGN and binds ubiquitinated proteins and membrane cargo molecules with a cytosolic acidic cluster-dileucine (AC-LL) motif. 
Sequence Annotation
 DOMAIN 16 146 VHS.
 DOMAIN 171 298 GAT.
 DOMAIN 594 715 GAE.
 REGION 1 313 Binds to ARF1 (in long isoform).
 REGION 299 593 Unstructured hinge.
 MOTIF 391 395 Autoinhibitory.  
Keyword
 3D-structure; Alternative splicing; Complete proteome; Endosome; Golgi apparatus; Membrane; Phosphoprotein; Polymorphism; Protein transport; Reference proteome; Transport; Ubl conjugation. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 723 AA 
Protein Sequence
MAEAEGESLE SWLNKATNPS NRQEDWEYII GFCDQINKEL EGPQIAVRLL AHKIQSPQEW 60
EALQALTVLE ACMKNCGRRF HNEVGKFRFL NELIKVVSPK YLGDRVSEKV KTKVIELLYS 120
WTMALPEEAK IKDAYHMLKR QGIVQSDPPI PVDRTLIPSP PPRPKNPVFD DEEKSKLLAK 180
LLKSKNPDDL QEANKLIKSM VKEDEARIQK VTKRLHTLEE VNNNVRLLSE MLLHYSQEDS 240
SDGDRELMKE LFDQCENKRR TLFKLASETE DNDNSLGDIL QASDNLSRVI NSYKTIIEGQ 300
VINGEVATLT LPDSEGNSQC SNQGTLIDLA ELDTTNSLSS VLAPAPTPPS SGIPILPPPP 360
QASGPPRSRS SSQAEATLGP SSTSNALSWL DEELLCLGLA DPAPNVPPKE SAGNSQWHLL 420
QREQSDLDFF SPRPGTAACG ASDAPLLQPS APSSSSSQAP LPPPFPAPVV PASVPAPSAG 480
SSLFSTGVAP ALAPKVEPAV PGHHGLALGN SALHHLDALD QLLEEAKVTS GLVKPTTSPL 540
IPTTTPARPL LPFSTGPGSP LFQPLSFQSQ GSPPKGPELS LASIHVPLES IKPSSALPVT 600
AYDKNGFRIL FHFAKECPPG RPDVLVVVVS MLNTAPLPVK SIVLQAAVPK SMKVKLQPPS 660
GTELSPFSPI QPPAAITQVM LLANPLKEKV RLRYKLTFAL GEQLSTEVGE VDQFPPVEQW 720
GNL 723 
Gene Ontology
 GO:0030131; C:clathrin adaptor complex; IEA:InterPro.
 GO:0010008; C:endosome membrane; IEA:UniProtKB-SubCell.
 GO:0005794; C:Golgi apparatus; IEA:UniProtKB-SubCell.
 GO:0005802; C:trans-Golgi network; IDA:UniProtKB.
 GO:0030306; F:ADP-ribosylation factor binding; IDA:UniProtKB.
 GO:0006886; P:intracellular protein transport; NAS:UniProtKB.
 GO:0016192; P:vesicle-mediated transport; IEA:InterPro. 
Interpro
 IPR008152; Clathrin_a/b/g-adaptin_app_Ig.
 IPR008153; Clathrin_g-adaptin_app.
 IPR013041; Coatomer/clathrin_app_Ig-like.
 IPR008942; ENTH_VHS.
 IPR004152; GAT.
 IPR027422; GGA3.
 IPR002014; VHS.
 IPR018205; VHS_subgr. 
Pfam
 PF02883; Alpha_adaptinC2
 PF03127; GAT
 PF00790; VHS 
SMART
 SM00809; Alpha_adaptinC2
 SM00288; VHS 
PROSITE
 PS50180; GAE
 PS50909; GAT
 PS50179; VHS 
PRINTS