CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-002034
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 DNA polymerase III subunit epsilon 
Protein Synonyms/Alias
  
Gene Name
 dnaQ 
Gene Synonyms/Alias
 mutD; b0215; JW0205 
Created Date
 July 27, 2013 
Organism
 Escherichia coli (strain K12) 
NCBI Taxa ID
 83333 
Lysine Modification
Position
Peptide
Type
References
53NNFHVYLKPDRLVDPacetylation[1]
Reference
 [1] Acetyl-Phosphate Is a Critical Determinant of Lysine Acetylation in E. coli.
 Weinert BT, Iesmantavicius V, Wagner SA, Schölz C, Gummesson B, Beli P, Nyström T, Choudhary C.
 Mol Cell. 2013 Jul 25;51(2):265-72. [PMID: 23830618] 
Functional Description
 DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. The epsilon subunit contain the editing function and is a proofreading 3'-5' exonuclease. 
Sequence Annotation
 ACT_SITE 162 162 Proton acceptor.
 METAL 12 12 Divalent metal cation 1; catalytic.
 METAL 12 12 Divalent metal cation 2; catalytic.
 METAL 14 14 Divalent metal cation 1; catalytic.
 METAL 167 167 Divalent metal cation 1; catalytic.
 BINDING 12 12 Substrate.
 BINDING 14 14 Substrate.
 BINDING 61 61 Substrate.
 BINDING 66 66 Substrate.
 BINDING 167 167 Substrate.  
Keyword
 3D-structure; Complete proteome; DNA replication; DNA-directed DNA polymerase; Exonuclease; Hydrolase; Magnesium; Manganese; Metal-binding; Nuclease; Nucleotidyltransferase; Reference proteome; Transferase. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 243 AA 
Protein Sequence
MSTAITRQIV LDTETTGMNQ IGAHYEGHKI IEIGAVEVVN RRLTGNNFHV YLKPDRLVDP 60
EAFGVHGIAD EFLLDKPTFA EVADEFMDYI RGAELVIHNA AFDIGFMDYE FSLLKRDIPK 120
TNTFCKVTDS LAVARKMFPG KRNSLDALCA RYEIDNSKRT LHGALLDAQI LAEVYLAMTG 180
GQTSMAFAME GETQQQQGEA TIQRIVRQAS KLRVVFATDE EIAAHEARLD LVQKKGGSCL 240
WRA 243 
Gene Ontology
 GO:0005622; C:intracellular; IEA:InterPro.
 GO:0003677; F:DNA binding; IEA:InterPro.
 GO:0003887; F:DNA-directed DNA polymerase activity; IEA:UniProtKB-KW.
 GO:0004527; F:exonuclease activity; IDA:EcoCyc.
 GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
 GO:0045004; P:DNA replication proofreading; IMP:EcoCyc. 
Interpro
 IPR006054; DnaQ.
 IPR006309; DnaQ_proteo.
 IPR006055; Exonuclease.
 IPR013520; Exonuclease_RNaseT/DNA_pol3.
 IPR012337; RNaseH-like_dom. 
Pfam
 PF00929; RNase_T 
SMART
 SM00479; EXOIII 
PROSITE
  
PRINTS