CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-000344
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 TATA-binding protein-associated factor 172 
Protein Synonyms/Alias
 ATP-dependent helicase BTAF1; B-TFIID transcription factor-associated 170 kDa subunit; TAF(II)170; TBP-associated factor 172; TAF-172 
Gene Name
 BTAF1 
Gene Synonyms/Alias
 TAF172 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
23GTTPVTRKAAAQQLGubiquitination[1, 2]
71QAVEAIVKNVPEWNPubiquitination[1]
430VINTLLPKVLTRIIEubiquitination[3, 4]
744AALQKECKAVTLAVQubiquitination[1]
1252EQLLDGKKLENYKIPubiquitination[3, 4]
1268PINAELRKYQQDGVNubiquitination[1]
1282NWLAFLNKYKLHGILubiquitination[1]
1467QFAARYGKPILASRDubiquitination[1]
1769EKIMGLQKFKMNIANubiquitination[1]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [3] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [4] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789] 
Functional Description
 Regulates transcription in association with TATA binding protein (TBP). Removes TBP from the TATA box in an ATP-dependent manner. 
Sequence Annotation
 REPEAT 385 422 HEAT 1.
 REPEAT 426 463 HEAT 2.
 REPEAT 513 550 HEAT 3.
 REPEAT 554 596 HEAT 4.
 REPEAT 818 855 HEAT 5.
 REPEAT 872 910 HEAT 6.
 REPEAT 1102 1139 HEAT 7.
 REPEAT 1182 1219 HEAT 8.
 DOMAIN 1278 1453 Helicase ATP-binding.
 DOMAIN 1636 1790 Helicase C-terminal.
 NP_BIND 1291 1298 ATP (Potential).
 MOTIF 191 207 Nuclear localization signal (Potential).
 MOTIF 1404 1407 DEGH box.  
Keyword
 ATP-binding; Complete proteome; Direct protein sequencing; DNA-binding; Helicase; Hydrolase; Nucleotide-binding; Nucleus; Reference proteome; Repeat. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1849 AA 
Protein Sequence
MAVSRLDRLF ILLDTGTTPV TRKAAAQQLG EVVKLHPHEL NNLLSKVLIY LRSANWDTRI 60
AAGQAVEAIV KNVPEWNPVP RTRQEPTSES SMEDSPTTER LNFDRFDICR LLQHGASLLG 120
SAGAEFEVQD EKSGEVDPKE RIARQRKLLQ KKLGLNMGEA IGMSTEELFN DEDLDYTPTS 180
ASFVNKQPTL QAAELIDSEF RAGMSNRQKN KAKRMAKLFA KQRSRDAVET NEKSNDSTDG 240
EPEEKRRKIA NVVINQSAND SKVLIDNIPD SSSLIEETNE WPLESFCEEL CNDLFNPSWE 300
VRHGAGTGLR EILKAHGKSG GKMGDSTLEE MIQQHQEWLE DLVIRLLCVF ALDRFGDFVS 360
DEVVAPVRET CAQTLGVVLK HMNETGVHKT VDVLLKLLTQ EQWEVRHGGL LGIKYALAVR 420
QDVINTLLPK VLTRIIEGLQ DLDDDVRAVA AASLVPVVES LVYLQTQKVP FIINTLWDAL 480
LELDDLTAST NSIMTLLSSL LTYPQVQQCS IQQSLTVLVP RVWPFLHHTI SSVRRAALET 540
LFTLLSTQDQ NSSSWLIPIL PDMLRHIFQF CVLESSQEIL DLIHKVWMEL LSKASVQYVV 600
AAACPWMGAW LCLMMQPSHL PIDLNMLLEV KARAKEKTGG KVRQGQSQNK EVLQEYIAGA 660
DTIMEDPATR DFVVMRARMM AAKLLGALCC CICDPGVNVV TQEIKPAESL GQLLLFHLNS 720
KSALQRISVA LVICEWAALQ KECKAVTLAV QPRLLDILSE HLYYDEIAVP FTRMQNECKQ 780
LISSLADVHI EVGNRVNNNV LTIDQASDLV TTVFNEATSS FDLNPQVLQQ LDSKRQQVQM 840
TVTETNQEWQ VLQLRVHTFA ACAVVSLQQL PEKLNPIIKP LMETIKKEEN TLVQNYAAQC 900
IAKLLQQCTT RTPCPNSKII KNLCSSLCVD PYLTPCVTCP VPTQSGQENS KGSTSEKDGM 960
HHTVTKHRGI ITLYRHQKAA FAITSRRGPT PKAVKAQIAD LPAGSSGNIL VELDEAQKPY 1020
LVQRRGAEFA LTTIVKHFGG EMAVKLPHLW DAMVGPLRNT IDINNFDGKS LLDKGDSPAQ 1080
ELVNSLQVFE TAAASMDSEL HPLLVQHLPH LYMCLQYPST AVRHMAARCV GVMSKIATME 1140
TMNIFLEKVL PWLGAIDDSV KQEGAIEALA CVMEQLDVGI VPYIVLLVVP VLGRMSDQTD 1200
SVRFMATQCF ATLIRLMPLE AGIPDPPNMS AELIQLKAKE RHFLEQLLDG KKLENYKIPV 1260
PINAELRKYQ QDGVNWLAFL NKYKLHGILC DDMGLGKTLQ SICILAGDHC HRAQEYARSK 1320
LAECMPLPSL VVCPPTLTGH WVDEVGKFCS REYLNPLHYT GPPTERIRLQ HQVKRHNLIV 1380
ASYDVVRNDI DFFRNIKFNY CILDEGHVIK NGKTKLSKAV KQLTANYRII LSGTPIQNNV 1440
LELWSLFDFL MPGFLGTERQ FAARYGKPIL ASRDARSSSR EQEAGVLAMD ALHRQVLPFL 1500
LRRMKEDVLQ DLPPKIIQDY YCTLSPLQVQ LYEDFAKSRA KCDVDETVSS ATLSEETEKP 1560
KLKATGHVFQ ALQYLRKLCN HPALVLTPQH PEFKTTAEKL AVQNSSLHDI QHAPKLSALK 1620
QLLLDCGLGN GSTSESGTES VVAQHRILIF CQLKSMLDIV EHDLLKPHLP SVTYLRLDGS 1680
IPPGQRHSIV SRFNNDPSID VLLLTTHVGG LGLNLTGADT VVFVEHDWNP MRDLQAMDRA 1740
HRIGQKRVVN VYRLITRGTL EEKIMGLQKF KMNIANTVIS QENSSLQSMG TDQLLDLFTL 1800
DKDGKAEKAD TSTSGKASMK SILENLSDLW DQEQYDSEYS LENFMHSLK 1849 
Gene Ontology
 GO:0005634; C:nucleus; NAS:UniProtKB.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
 GO:0004386; F:helicase activity; IEA:UniProtKB-KW.
 GO:0003700; F:sequence-specific DNA binding transcription factor activity; NAS:UniProtKB.
 GO:0045892; P:negative regulation of transcription, DNA-dependent; NAS:UniProtKB. 
Interpro
 IPR011989; ARM-like.
 IPR016024; ARM-type_fold.
 IPR022707; DUF3535.
 IPR014001; Helicase_ATP-bd.
 IPR001650; Helicase_C.
 IPR027417; P-loop_NTPase.
 IPR000330; SNF2_N. 
Pfam
 PF12054; DUF3535
 PF00271; Helicase_C
 PF00176; SNF2_N 
SMART
 SM00487; DEXDc
 SM00490; HELICc 
PROSITE
 PS50077; HEAT_REPEAT
 PS51192; HELICASE_ATP_BIND_1
 PS51194; HELICASE_CTER 
PRINTS