UniProt Accession

 TIM9_HUMAN; Q9Y5J7; B2R584 

Genbank Protein ID

 AF150100; AF152353; AK312095; CH471061; BC020213; BC054875 

Genbank Nucleotide ID

 AAD40006.1; AAF15103.1; BAG35031.1; EAW80735.1; AAH20213.1; AAH54875.1 

Protein Name

 Mitochondrial import inner membrane translocase subunit Tim9 

Protein Synonyms/Alias

  

Gene Name

 TIMM9 

Gene Synonyms/Alias

 TIM9; TIM9A; TIMM9A 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
34	TCFLDCVKDFTTREV	ubiquitination	[1]
81	QNEALAAKAGLLGQP	ubiquitination	[1, 2, 3, 4]

Reference

 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [3] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [4] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789] 

Functional Description

 Mitochondrial intermembrane chaperone that participates in the import and insertion of multi-pass transmembrane proteins into the mitochondrial inner membrane. May also be required for the transfer of beta-barrel precursors from the TOM complex to the sorting and assembly machinery (SAM complex) of the outer membrane. Acts as a chaperone-like protein that protects the hydrophobic precursors from aggregation and guide them through the mitochondrial intermembrane space. 

Sequence Annotation

 MOTIF 28 52 Twin CX3C motif.
 MOD_RES 2 2 N-acetylalanine.
 DISULFID 28 52
 DISULFID 32 48  

Keyword

 3D-structure; Acetylation; Chaperone; Complete proteome; Disulfide bond; Membrane; Metal-binding; Mitochondrion; Mitochondrion inner membrane; Protein transport; Reference proteome; Translocation; Transport; Zinc. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 89 AA 

Protein Sequence

Gene Ontology

 GO:0005743; C:mitochondrial inner membrane; IDA:BHF-UCL.
 GO:0042719; C:mitochondrial intermembrane space protein transporter complex; IDA:BHF-UCL.
 GO:0005215; F:transporter activity; IDA:BHF-UCL.
 GO:0008270; F:zinc ion binding; TAS:ProtInc.
 GO:0044267; P:cellular protein metabolic process; TAS:Reactome.
 GO:0072321; P:chaperone-mediated protein transport; IDA:BHF-UCL.
 GO:0045039; P:protein import into mitochondrial inner membrane; IDA:BHF-UCL.
 GO:0007605; P:sensory perception of sound; TAS:ProtInc. 

Interpro

 IPR004217; Tim10/DDP_fam_Znf. 

Pfam

 PF02953; zf-Tim10_DDP 

SMART

  

PROSITE

  

PRINTS