UniProt Accession

 IMA5_HUMAN; P52294; D3DN93; Q6IBQ9; Q9BQ56 

Genbank Protein ID

 S75295; BT006959; CR456743; AC083798; AC096861; CH471052; CH471052; BC002374; BC003009 

Genbank Nucleotide ID

 AAC60648.1; AAP35605.1; CAG33024.1; EAW79482.1; EAW79483.1; AAH02374.1; AAH03009.1 

Protein Name

 Importin subunit alpha-5 

Protein Synonyms/Alias

 Karyopherin subunit alpha-1; Nucleoprotein interactor 1; NPI-1; RAG cohort protein 2; SRP1-beta 

Gene Name

 KPNA1 

Gene Synonyms/Alias

 RCH2 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
6	**MTTPGKENFRLKS	ubiquitination	[1]
46	KREEQLFKRRNVATA	ubiquitination	[2]
105	QQLSATQKFRKLLSK	ubiquitination	[1, 2, 3]
112	KFRKLLSKEPNPPID	ubiquitination	[1]
136	ARFVEFLKRKENCTL	ubiquitination	[2, 3]
355	SSPKESIKKEACWTI	ubiquitination	[1]
356	SPKESIKKEACWTIS	ubiquitination	[1, 2, 4]
398	TAEFRTRKEAAWAIT	ubiquitination	[1, 3]
459	RLGEQEAKRNGTGIN	ubiquitination	[3]

Reference

 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [3] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [4] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094] 

Functional Description

 Functions in nuclear protein import as an adapter protein for nuclear receptor KPNB1. Binds specifically and directly to substrates containing either a simple or bipartite NLS motif. Docking of the importin/substrate complex to the nuclear pore complex (NPC) is mediated by KPNB1 through binding to nucleoporin FxFG repeats and the complex is subsequently translocated through the pore by an energy requiring, Ran- dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to importin-beta and the three components separate and importin-alpha and -beta are re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran from importin. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. In vitro, mediates the nuclear import of human cytomegalovirus UL84 by recognizing a non- classical NLS. 

Sequence Annotation

 DOMAIN 1 57 IBB.
 REPEAT 77 117 ARM 1; truncated.
 REPEAT 118 161 ARM 2.
 REPEAT 162 206 ARM 3.
 REPEAT 207 245 ARM 4.
 REPEAT 246 290 ARM 5.
 REPEAT 291 330 ARM 6.
 REPEAT 331 372 ARM 7.
 REPEAT 373 412 ARM 8.
 REPEAT 413 457 ARM 9.
 REPEAT 460 504 ARM 10; atypical.
 REGION 149 241 NLS binding site (major) (By similarity).
 REGION 245 437 Binding to RAG1.
 REGION 318 406 NLS binding site (minor) (By similarity).
 MOTIF 42 51 Nuclear localization signal (By
 MOD_RES 3 3 Phosphothreonine (By similarity).  

Keyword

 3D-structure; Complete proteome; Cytoplasm; Direct protein sequencing; Host-virus interaction; Nucleus; Phosphoprotein; Polymorphism; Protein transport; Reference proteome; Repeat; Transport; Ubl conjugation. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 538 AA 

Protein Sequence

Gene Ontology

 GO:0005829; C:cytosol; TAS:Reactome.
 GO:0030425; C:dendrite; ISS:UniProtKB.
 GO:0005643; C:nuclear pore; TAS:ProtInc.
 GO:0005654; C:nucleoplasm; TAS:Reactome.
 GO:0008139; F:nuclear localization sequence binding; TAS:ProtInc.
 GO:0008565; F:protein transporter activity; IEA:InterPro.
 GO:0006309; P:apoptotic DNA fragmentation; TAS:Reactome.
 GO:0019221; P:cytokine-mediated signaling pathway; TAS:Reactome.
 GO:0075733; P:intracellular transport of viral material; TAS:Reactome.
 GO:0006607; P:NLS-bearing substrate import into nucleus; TAS:ProtInc.
 GO:0042307; P:positive regulation of protein import into nucleus; ISS:UniProtKB.
 GO:0000018; P:regulation of DNA recombination; TAS:ProtInc.
 GO:0019058; P:viral infectious cycle; TAS:Reactome. 

Interpro

 IPR011989; ARM-like.
 IPR016024; ARM-type_fold.
 IPR000225; Armadillo.
 IPR002652; Importin-a_IBB.
 IPR024931; Importing_su_alpha. 

Pfam

 PF00514; Arm
 PF01749; IBB 

SMART

 SM00185; ARM 

PROSITE

 PS50176; ARM_REPEAT
 PS51214; IBB 

PRINTS