CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-024708
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Scaffold attachment factor B1 
Protein Synonyms/Alias
 SAF-B1 
Gene Name
 Safb 
Gene Synonyms/Alias
 Safb1 
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
54NLDSSGNKSVLMERLacetylation[1]
316DAPLAVVKREPADAPacetylation[1]
378DSKEDVKKFAFDACNacetylation[1, 2]
445TTRATDLKNLFSRYGubiquitination[3]
458YGKVVGAKVVTNARSacetylation[1]
458YGKVVGAKVVTNARSubiquitination[3]
497HKTELHGKMISVEKAacetylation[1, 2]
629RSVVSFDKVKESRKSacetylation[1, 2]
Reference
 [1] Quantitative acetylome analysis reveals the roles of SIRT1 in regulating diverse substrates and cellular pathways.
 Chen Y, Zhao W, Yang JS, Cheng Z, Luo H, Lu Z, Tan M, Gu W, Zhao Y.
 Mol Cell Proteomics. 2012 Oct;11(10):1048-62. [PMID: 22826441]
 [2] SIRT5-Mediated Lysine Desuccinylation Impacts Diverse Metabolic Pathways.
 Park J, Chen Y, Tishkoff DX, Peng C, Tan M, Dai L, Xie Z, Zhang Y, Zwaans BM, Skinner ME, Lombard DB, Zhao Y.
 Mol Cell. 2013 Jun 27;50(6):919-30. [PMID: 23806337]
 [3] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023] 
Functional Description
 Binds to scaffold/matrix attachment region (S/MAR) DNA and forms a molecular assembly point to allow the formation of a 'transcriptosomal' complex (consisting of SR proteins and RNA polymerase II) coupling transcription and RNA processing. Can function as an estrogen receptor corepressor and can also bind to the HSP27 promoter and decrease its transcription. Can inhibit cell proliferation. When associated with RBMX, binds to and stimulates transcription from the SREBF1 promoter. 
Sequence Annotation
 DOMAIN 31 65 SAP.
 DOMAIN 428 506 RRM.
 REGION 550 814 Interaction with POLR2A (By similarity).
 REGION 621 937 Interaction with SAFB2 (By similarity).
 MOTIF 621 638 Nuclear localization signal (Potential).
 MOD_RES 2 2 N-acetylalanine (By similarity).
 MOD_RES 55 55 Phosphoserine (By similarity).
 MOD_RES 405 405 Phosphoserine (By similarity).
 MOD_RES 406 406 Phosphoserine (By similarity).
 MOD_RES 437 437 Phosphoserine (By similarity).
 MOD_RES 602 602 Phosphoserine (By similarity).
 MOD_RES 604 604 Phosphoserine (By similarity).
 MOD_RES 623 623 Phosphoserine (By similarity).
 MOD_RES 626 626 Phosphoserine (By similarity).
 MOD_RES 629 629 N6-acetyllysine (By similarity).
 CROSSLNK 231 231 Glycyl lysine isopeptide (Lys-Gly)
 CROSSLNK 316 316 Glycyl lysine isopeptide (Lys-Gly)  
Keyword
 Acetylation; Coiled coil; Complete proteome; DNA-binding; Isopeptide bond; Nucleus; Phosphoprotein; Reference proteome; Repressor; RNA-binding; Transcription; Transcription regulation; Ubl conjugation. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 937 AA 
Protein Sequence
MAETLSGLGD ASAAGAAAVS SAASETGTRR LSDLRVIDLR AELKKRNLDS SGNKSVLMER 60
LKKAIEDEGG NPDEIEVTSE CNKKMPKRPS KGRKPEDEGV EDNGLEENSG DGQEDVETSL 120
ENLQDMDMMD ISVLDEADID NGSVADCVEE EEEATLPEGL ADSTELVEGD LKGLPEQLQE 180
HAIDDKDTVN NVDTSSSDFT MLQEMEEASL EPENEKILDI LGETCKSEPV KEEGSELEQP 240
FAQATSSVGP DRKLAEEEDL FESCGHPEEE EEEEEEDQEE EQEEEGDLAL ASSSKSESPS 300
TRCQWSEADA PLAVVKREPA DAPGGGTGMD REPVGLEEPV EQSSTAAQLP EATSQELVRA 360
PTAALSPEPQ DSKEDVKKFA FDACNDVPAP PKESSASEGA DQKMSSVEED SDTKRLSREE 420
KGRSSCGRNF WVSGLSSTTR ATDLKNLFSR YGKVVGAKVV TNARSPGARC YGFVTMSTAE 480
EATKCISHLH KTELHGKMIS VEKAKSEPTG KRVPDRRDGD SKKEKASTSD RSANLKREEK 540
GERKDDAKKT DDGSTEKSKD ADDQKPGPSE RSRTTKSGSR GTERTVVMDK SKGVPVISVK 600
TSGSKERASK SQDRKSASRE KRSVVSFDKV KESRKSRDSE SRRERERSER EQRLQAQWER 660
EERERLEIAR ERLAFHRHRL ERERMERERL ERERMHVEQE RRREQERIHR EREELRRQQE 720
LRYEQERRPA VRRPYEVDGR RDDAYWPEAK RAALDDRYHS DFSRQDRFHD FDHRDRGRYP 780
NHSVDRREGS RSMMGDREGQ HYPERHGGPE RHGRDSRDGW GYGSNKRLSE GRGLPPPPRR 840
DWGEHGRRLE DDRAWQGTAD GGMMERDHKR WQGGERSMSG HSGPGHMMNR GGMSGRGSFA 900
PGGASRGHVI PRGGMQAGFG GQSRGSRPSD ARFTRRY 937 
Gene Ontology
 GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
 GO:0003682; F:chromatin binding; IDA:UniProtKB.
 GO:0001047; F:core promoter binding; IDA:UniProtKB.
 GO:0000166; F:nucleotide binding; IEA:InterPro.
 GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
 GO:0040007; P:growth; IMP:MGI.
 GO:0042445; P:hormone metabolic process; IMP:MGI.
 GO:0030520; P:intracellular estrogen receptor signaling pathway; IMP:MGI.
 GO:0045944; P:positive regulation of transcription from RNA polymerase II promoter; IMP:UniProtKB.
 GO:0006351; P:transcription, DNA-dependent; IEA:UniProtKB-KW. 
Interpro
 IPR012677; Nucleotide-bd_a/b_plait.
 IPR000504; RRM_dom.
 IPR003034; SAP_dom. 
Pfam
 PF02037; SAP 
SMART
 SM00360; RRM
 SM00513; SAP 
PROSITE
 PS50102; RRM
 PS50800; SAP 
PRINTS