UniProt Accession

 ASF1_YEAST; P32447; D6VW69 

Genbank Protein ID

 L07593; Z49390; AY557874; BK006943 

Genbank Nucleotide ID

 AAC37512.1; CAA89410.1; AAS56200.1; DAA08685.1 

Protein Name

 Histone chaperone ASF1 

Protein Synonyms/Alias

 Anti-silencing function protein 1; yASF1 

Gene Name

 ASF1 

Gene Synonyms/Alias

 CIA1; YJL115W; J0755 

Created Date

 July 27, 2013 

Organism

 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 

NCBI Taxa ID

 559292 

Lysine Modification

Position	Peptide	Type	References
259	GNDKKRRKIEGGSTD	acetylation	[1]

Reference

 [1] Proteome-wide analysis of lysine acetylation suggests its broad regulatory scope in Saccharomyces cerevisiae.
 Henriksen P, Wagner SA, Weinert BT, Sharma S, Bacinskaja G, Rehman M, Juffer AH, Walther TC, Lisby M, Choudhary C.
 Mol Cell Proteomics. 2012 Nov;11(11):1510-22. [PMID: 22865919] 

Functional Description

 Histone chaperone that facilitates histone deposition and histone exchange and removal during nucleosome assembly and disassembly. Facilitates histone deposition through both replication-dependent and replication-independent chromatin assembly pathways. Cooperates with chromatin assembly factor 1 (CAF-1) to promote replication-dependent chromatin assembly and with the HIR complex to promote replication-independent chromatin assembly, which may occur during transcription and DNA repair. May be required for the maintenance of a subset of replication elongation factors, including DNA polymerase epsilon, the RFC complex and PCNA, at stalled replication forks. Also required for acetylation of histone H3 on 'Lys-9' and 'Lys-56'. 

Sequence Annotation

 REGION 1 155 Interaction with histone H3, histone H4,
 REGION 1 143 Interaction with HIR1.  

Keyword

 3D-structure; Chaperone; Chromatin regulator; Coiled coil; Complete proteome; Nucleus; Reference proteome; Transcription; Transcription regulation. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 279 AA 

Protein Sequence

Gene Ontology

 GO:0005829; C:cytosol; IDA:SGD.
 GO:0005634; C:nucleus; IDA:SGD.
 GO:0042393; F:histone binding; IMP:SGD.
 GO:0030466; P:chromatin silencing at silent mating-type cassette; IGI:SGD.
 GO:0006348; P:chromatin silencing at telomere; IGI:SGD.
 GO:0006335; P:DNA replication-dependent nucleosome assembly; IDA:SGD.
 GO:0006336; P:DNA replication-independent nucleosome assembly; IDA:SGD.
 GO:0016573; P:histone acetylation; IMP:SGD.
 GO:0043486; P:histone exchange; IMP:SGD.
 GO:0006337; P:nucleosome disassembly; IMP:SGD.
 GO:0035066; P:positive regulation of histone acetylation; IDA:SGD.
 GO:0032968; P:positive regulation of transcription elongation from RNA polymerase II promoter; IDA:SGD.
 GO:0043618; P:regulation of transcription from RNA polymerase II promoter in response to stress; IMP:SGD.
 GO:0006351; P:transcription, DNA-dependent; IEA:UniProtKB-KW. 

Interpro

 IPR017282; Hist_deposition_Asf1.
 IPR006818; Histone_chaperone_ASF1-like. 

Pfam

 PF04729; ASF1_hist_chap 

SMART

  

PROSITE

  

PRINTS