CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-000464
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Synaptobrevin homolog YKT6 
Protein Synonyms/Alias
  
Gene Name
 YKT6 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
103KVLDEFSKQVDRIDWubiquitination[1, 2]
141READPMTKVQAELDEubiquitination[2]
150QAELDETKIILHNTMubiquitination[2]
166SLLERGEKLDDLVSKacetylation[3]
173KLDDLVSKSEVLGTQubiquitination[2]
182EVLGTQSKAFYKTARubiquitination[1]
186TQSKAFYKTARKQNSubiquitination[4]
Reference
 [1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [3] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
 Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
 PLoS One. 2012;7(12):e50545. [PMID: 23236377]
 [4] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961] 
Functional Description
 Vesicular soluble NSF attachment protein receptor (v- SNARE) mediating vesicle docking and fusion to a specific acceptor cellular compartment. Functions in endoplasmic reticulum to Golgi transport; as part of a SNARE complex composed of GOSR1, GOSR2 and STX5. Functions in early/recycling endosome to TGN transport; as part of a SNARE complex composed of BET1L, GOSR1 and STX5. Has a S-palmitoyl transferase activity. 
Sequence Annotation
 DOMAIN 8 126 Longin.
 DOMAIN 138 198 v-SNARE coiled-coil homology.
 MOD_RES 195 195 Cysteine methyl ester (Probable).
 LIPID 194 194 S-palmitoyl cysteine.
 LIPID 195 195 S-farnesyl cysteine.  
Keyword
 Coiled coil; Complete proteome; Cytoplasm; Cytoplasmic vesicle; ER-Golgi transport; Golgi apparatus; Lipoprotein; Membrane; Methylation; Palmitate; Prenylation; Protein transport; Reference proteome; Transferase; Transport. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 198 AA 
Protein Sequence
MKLYSLSVLY KGEAKVVLLK AAYDVSSFSF FQRSSVQEFM TFTSQLIVER SSKGTRASVK 60
EQDYLCHVYV RNDSLAGVVI ADNEYPSRVA FTLLEKVLDE FSKQVDRIDW PVGSPATIHY 120
PALDGHLSRY QNPREADPMT KVQAELDETK IILHNTMESL LERGEKLDDL VSKSEVLGTQ 180
SKAFYKTARK QNSCCAIM 198 
Gene Ontology
 GO:0030659; C:cytoplasmic vesicle membrane; IEA:UniProtKB-SubCell.
 GO:0005829; C:cytosol; IEA:UniProtKB-SubCell.
 GO:0005783; C:endoplasmic reticulum; IDA:HGNC.
 GO:0005768; C:endosome; IDA:HGNC.
 GO:0005794; C:Golgi apparatus; IDA:HGNC.
 GO:0000139; C:Golgi membrane; IEA:UniProtKB-SubCell.
 GO:0005887; C:integral to plasma membrane; IDA:HGNC.
 GO:0005739; C:mitochondrion; IDA:HPA.
 GO:0031201; C:SNARE complex; ISS:HGNC.
 GO:0019706; F:protein-cysteine S-palmitoleyltransferase activity; IDA:HGNC.
 GO:0005484; F:SNAP receptor activity; IDA:HGNC.
 GO:0006888; P:ER to Golgi vesicle-mediated transport; IDA:HGNC.
 GO:0015031; P:protein transport; IEA:UniProtKB-KW.
 GO:0042147; P:retrograde transport, endosome to Golgi; IDA:HGNC.
 GO:0006904; P:vesicle docking involved in exocytosis; IDA:HGNC.
 GO:0006903; P:vesicle targeting; IDA:HGNC. 
Interpro
 IPR011012; Longin-like_dom.
 IPR010908; Longin_dom.
 IPR001388; Synaptobrevin. 
Pfam
 PF13774; Longin
 PF00957; Synaptobrevin 
SMART
  
PROSITE
 PS50859; LONGIN
 PS00417; SYNAPTOBREVIN
 PS50892; V_SNARE 
PRINTS