CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-001383
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Ubiquitin carboxyl-terminal hydrolase 2 
Protein Synonyms/Alias
 41 kDa ubiquitin-specific protease; Deubiquitinating enzyme 2; Ubiquitin thioesterase 2; Ubiquitin-specific-processing protease 2 
Gene Name
 Usp2 
Gene Synonyms/Alias
 Ubp41; MNCb-0190 
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
528ESRIRTSKLTTFVNFubiquitination[1]
Reference
 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023] 
Functional Description
 Hydrolase that deubiquitinates polyubiquitinated target proteins such as MDM2, MDM4 and CCND1. Isoform 1 and isoform 2 possess both ubiquitin-specific peptidase and isopeptidase activities. Deubiquitinates MDM2 without reversing MDM2-mediated p53/TP53 ubiquitination and thus indirectly promotes p53/TP53 degradation and limits p53 activity. Has no deubiquitinase activity against p53/TP53. Prevents MDM2-mediated degradation of MDM4. Plays a role in the G1/S cell-cycle progression in normal and cancer cells. Plays a role in the regulation of myogenic differentiation of embryonic muscle cells (By similarity). 
Sequence Annotation
 REGION 1 207 Necessary for interaction with MDM4 (By
 REGION 411 511 Necessary for interaction with MDM4 (By
 ACT_SITE 284 284 Nucleophile (By similarity).
 ACT_SITE 565 565 Proton acceptor (By similarity).
 METAL 433 433 Zinc (By similarity).
 METAL 436 436 Zinc (By similarity).
 METAL 484 484 Zinc (By similarity).
 METAL 487 487 Zinc (By similarity).  
Keyword
 Alternative splicing; Cell cycle; Complete proteome; Cytoplasm; Hydrolase; Metal-binding; Myogenesis; Nucleus; Protease; Reference proteome; Thiol protease; Ubl conjugation pathway; Zinc. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 613 AA 
Protein Sequence
MSQLSSTLKR YTESSRYTDA PYAKPGYGTY TPSSYGANLA ASFLEKEKLG FKPVSPTSFL 60
PRPRTYGPSS ILDCDRGRPL LRSDIIGSSK RSESQTRGNE RPSGSGLNGG SGFSYGVSSN 120
SLSYLPMNAR DQGVTLSQKK SNSQSDLARD FSSLRTSDGY RTSDGYRTSE GFRIDPGNLG 180
RSPMLARTRK ELCALQGLYQ AASRSEYLTD YLENYGRKGS APQVLTQAPP PSRVPEVLSP 240
TYRPSGRYTL WEKSKGQASG PSRSSSPGRD TMNSKSAQGL AGLRNLGNTC FMNSILQCLS 300
NTRELRDYCL QRLYMRDLGH TSSAHTALME EFAKLIQTIW TSSPNDVVSP SEFKTQIQRY 360
APRFMGYNQQ DAQEFLRFLL DGLHNEVNRV AARPKASPET LDHLPDEEKG RQMWRKYLER 420
EDSRIGDLFV GQLKSSLTCT DCGYCSTVFD PFWDLSLPIA KRGYPEVTLM DCMRLFTKED 480
ILDGDEKPTC CRCRARKRCI KKFSVQRFPK ILVLHLKRFS ESRIRTSKLT TFVNFPLRDL 540
DLREFASENT NHAVYNLYAV SNHSGTTMGG HYTAYCRSPV TGEWHTFNDS SVTPMSSSQV 600
RTSDAYLLFY ELASPPSRM 619 
Gene Ontology
 GO:0005938; C:cell cortex; IDA:MGI.
 GO:0005813; C:centrosome; IEA:Compara.
 GO:0005737; C:cytoplasm; IDA:MGI.
 GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
 GO:0048471; C:perinuclear region of cytoplasm; IDA:MGI.
 GO:0008234; F:cysteine-type peptidase activity; IEA:UniProtKB-KW.
 GO:0042802; F:identical protein binding; IDA:MGI.
 GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
 GO:0004221; F:ubiquitin thiolesterase activity; ISS:UniProtKB.
 GO:0007049; P:cell cycle; IEA:UniProtKB-KW.
 GO:0007517; P:muscle organ development; IEA:UniProtKB-KW.
 GO:0048642; P:negative regulation of skeletal muscle tissue development; IEA:Compara.
 GO:0000122; P:negative regulation of transcription from RNA polymerase II promoter; ISS:UniProtKB.
 GO:0045931; P:positive regulation of mitotic cell cycle; ISS:UniProtKB.
 GO:0048643; P:positive regulation of skeletal muscle tissue development; IEA:Compara.
 GO:0016579; P:protein deubiquitination; ISS:UniProtKB.
 GO:0050821; P:protein stabilization; ISS:UniProtKB.
 GO:0006511; P:ubiquitin-dependent protein catabolic process; IEA:InterPro. 
Interpro
 IPR018200; Pept_C19ubi-hydrolase_C_CS.
 IPR001394; Peptidase_C19. 
Pfam
 PF00443; UCH 
SMART
  
PROSITE
 PS00972; UCH_2_1
 PS00973; UCH_2_2
 PS50235; UCH_2_3 
PRINTS