UniProt Accession

 RAB8A_HUMAN; P61006; P24407; Q6FHV5 

Genbank Protein ID

 X56741; S53268; AF498943; BT007184; CR536583; CR542274; CH471106; BC002977 

Genbank Nucleotide ID

 CAA40065.1; AAB19681.1; AAM21091.1; AAP35848.1; CAG38820.1; CAG47070.1; EAW84526.1; AAH02977.1 

Protein Name

 Ras-related protein Rab-8A 

Protein Synonyms/Alias

 Oncogene c-mel 

Gene Name

 RAB8A 

Gene Synonyms/Alias

 MEL; RAB8 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
3	*****MAKTYDYLFK	acetylation	[1]
3	*****MAKTYDYLFK	ubiquitination	[2, 3]
116	HASADVEKMILGNKC	ubiquitination	[4]
138	VSKERGEKLALDYGI	ubiquitination	[2, 4]
190	QGSNQGVKITPDQQK	ubiquitination	[4, 5]

Reference

 [1] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
 Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
 Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861]
 [2] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
 [3] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [4] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [5] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094] 

Functional Description

 May be involved in vesicular trafficking and neurotransmitter release. Together with RAB11A, RAB3IP, the exocyst complex, PARD3, PRKCI, ANXA2, CDC42 and DNMBP promotes transcytosis of PODXL to the apical membrane initiation sites (AMIS), apical surface formation and lumenogenesis. Together with MYO5B and RAB11A participates in epithelial cell polarization. Plays an important role in ciliogenesis. 

Sequence Annotation

 NP_BIND 15 22 GTP (By similarity).
 NP_BIND 63 67 GTP (By similarity).
 NP_BIND 121 124 GTP (By similarity).
 MOTIF 37 45 Effector region (By similarity).
 MOD_RES 181 181 Phosphoserine.
 MOD_RES 185 185 Phosphoserine.
 MOD_RES 204 204 Cysteine methyl ester (Potential).
 LIPID 204 204 S-geranylgeranyl cysteine.  

Keyword

 3D-structure; Cell membrane; Cell projection; Cilium biogenesis/degradation; Complete proteome; Cytoplasm; Cytoplasmic vesicle; Golgi apparatus; GTP-binding; Lipoprotein; Membrane; Methylation; Nucleotide-binding; Phosphoprotein; Prenylation; Protein transport; Proto-oncogene; Reference proteome; Transport. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 207 AA 

Protein Sequence

Gene Ontology

 GO:0030659; C:cytoplasmic vesicle membrane; TAS:Reactome.
 GO:0000139; C:Golgi membrane; TAS:Reactome.
 GO:0005932; C:microtubule basal body; IEA:Compara.
 GO:0043025; C:neuronal cell body; IEA:Compara.
 GO:0031513; C:nonmotile primary cilium; IDA:BHF-UCL.
 GO:0048471; C:perinuclear region of cytoplasm; IEA:UniProtKB-SubCell.
 GO:0045335; C:phagocytic vesicle; IDA:UniProtKB.
 GO:0030670; C:phagocytic vesicle membrane; IEA:UniProtKB-SubCell.
 GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
 GO:0014069; C:postsynaptic density; IEA:Compara.
 GO:0055037; C:recycling endosome; IEA:Compara.
 GO:0019003; F:GDP binding; IDA:UniProtKB.
 GO:0005525; F:GTP binding; IDA:UniProtKB.
 GO:0003924; F:GTPase activity; EXP:Reactome.
 GO:0017137; F:Rab GTPase binding; ISS:UniProtKB.
 GO:0007409; P:axonogenesis; ISS:UniProtKB.
 GO:0042384; P:cilium assembly; IMP:UniProtKB.
 GO:0000086; P:G2/M transition of mitotic cell cycle; TAS:Reactome.
 GO:0048210; P:Golgi vesicle fusion to target membrane; IDA:BHF-UCL.
 GO:0015031; P:protein transport; IEA:UniProtKB-KW.
 GO:0048169; P:regulation of long-term neuronal synaptic plasticity; IEA:Compara.
 GO:0051223; P:regulation of protein transport; IEA:Compara.
 GO:0007264; P:small GTPase mediated signal transduction; IEA:InterPro.
 GO:0006904; P:vesicle docking involved in exocytosis; IDA:BHF-UCL. 

Interpro

 IPR027417; P-loop_NTPase.
 IPR005225; Small_GTP-bd_dom.
 IPR001806; Small_GTPase.
 IPR003579; Small_GTPase_Rab_type. 

Pfam

 PF00071; Ras 

SMART

 SM00175; RAB 

PROSITE

 PS51419; RAB 

PRINTS

 PR00449; RASTRNSFRMNG.