CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-008451
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 CDK-activating kinase assembly factor MAT1 
Protein Synonyms/Alias
 CDK7/cyclin-H assembly factor; Cyclin-G1-interacting protein; Menage a trois; RING finger protein 66; RING finger protein MAT1; p35; p36 
Gene Name
 MNAT1 
Gene Synonyms/Alias
 CAP35; MAT1; RNF66 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
167QRRLFIQKEEQLQQIubiquitination[1, 2, 3, 4]
180QILKRKNKQAFLDELubiquitination[3]
Reference
 [1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [2] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
 [3] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [4] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789] 
Functional Description
 Stabilizes the cyclin H-CDK7 complex to form a functional CDK-activating kinase (CAK) enzymatic complex. CAK activates the cyclin-associated kinases CDK1, CDK2, CDK4 and CDK6 by threonine phosphorylation. CAK complexed to the core-TFIIH basal transcription factor activates RNA polymerase II by serine phosphorylation of the repetitive C-terminus domain (CTD) of its large subunit (POLR2A), allowing its escape from the promoter and elongation of the transcripts. Involved in cell cycle control and in RNA transcription by RNA polymerase II. 
Sequence Annotation
 REPEAT 142 161 UIM.
 ZN_FING 6 50 RING-type.
 MOD_RES 1 1 N-acetylmethionine.
 MOD_RES 51 51 Phosphothreonine.
 MOD_RES 279 279 Phosphoserine.  
Keyword
 3D-structure; Acetylation; Alternative splicing; Cell cycle; Complete proteome; Direct protein sequencing; Metal-binding; Nucleus; Phosphoprotein; Polymorphism; Reference proteome; Transcription; Transcription regulation; Zinc; Zinc-finger. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 309 AA 
Protein Sequence
MDDQGCPRCK TTKYRNPSLK LMVNVCGHTL CESCVDLLFV RGAGNCPECG TPLRKSNFRV 60
QLFEDPTVDK EVEIRKKVLK IYNKREEDFP SLREYNDFLE EVEEIVFNLT NNVDLDNTKK 120
KMEIYQKENK DVIQKNKLKL TREQEELEEA LEVERQENEQ RRLFIQKEEQ LQQILKRKNK 180
QAFLDELESS DLPVALLLAQ HKDRSTQLEM QLEKPKPVKP VTFSTGIKMG QHISLAPIHK 240
LEEALYEYQP LQIETYGPHV PELEMLGRLG YLNHVRAASP QDLAGGYTSS LACHRALQDA 300
FSGLFWQPS 309 
Gene Ontology
 GO:0005737; C:cytoplasm; IDA:HPA.
 GO:0005675; C:holo TFIIH complex; IDA:UniProtKB.
 GO:0008270; F:zinc ion binding; TAS:ProtInc.
 GO:0006370; P:7-methylguanosine mRNA capping; TAS:Reactome.
 GO:0007512; P:adult heart development; IEA:Compara.
 GO:0006915; P:apoptotic process; IEA:Compara.
 GO:0008283; P:cell proliferation; TAS:ProtInc.
 GO:0000082; P:G1/S transition of mitotic cell cycle; TAS:Reactome.
 GO:0000086; P:G2/M transition of mitotic cell cycle; TAS:Reactome.
 GO:0044236; P:multicellular organismal metabolic process; IEA:Compara.
 GO:0043066; P:negative regulation of apoptotic process; IEA:Compara.
 GO:0000718; P:nucleotide-excision repair, DNA damage removal; TAS:Reactome.
 GO:0048661; P:positive regulation of smooth muscle cell proliferation; IEA:Compara.
 GO:0045944; P:positive regulation of transcription from RNA polymerase II promoter; IDA:UniProtKB.
 GO:0050434; P:positive regulation of viral transcription; TAS:Reactome.
 GO:0006461; P:protein complex assembly; TAS:ProtInc.
 GO:0000079; P:regulation of cyclin-dependent protein serine/threonine kinase activity; TAS:ProtInc.
 GO:0051592; P:response to calcium ion; IEA:Compara.
 GO:0006363; P:termination of RNA polymerase I transcription; TAS:Reactome.
 GO:0006362; P:transcription elongation from RNA polymerase I promoter; TAS:Reactome.
 GO:0006368; P:transcription elongation from RNA polymerase II promoter; TAS:Reactome.
 GO:0006361; P:transcription initiation from RNA polymerase I promoter; TAS:Reactome.
 GO:0006367; P:transcription initiation from RNA polymerase II promoter; TAS:Reactome.
 GO:0006283; P:transcription-coupled nucleotide-excision repair; TAS:Reactome.
 GO:0021591; P:ventricular system development; IEA:Compara.
 GO:0022415; P:viral reproductive process; TAS:Reactome. 
Interpro
 IPR015877; Cdk-activating_kinase_MAT1_cen.
 IPR004575; MAT1/Tfb3.
 IPR003903; Ubiquitin-int_motif.
 IPR001841; Znf_RING.
 IPR013083; Znf_RING/FYVE/PHD.
 IPR017907; Znf_RING_CS. 
Pfam
 PF06391; MAT1 
SMART
 SM00184; RING 
PROSITE
 PS50330; UIM
 PS00518; ZF_RING_1
 PS50089; ZF_RING_2 
PRINTS