UniProt Accession

 HSP7C_RAT; P63018; P08109; P12225; Q4FZY7; Q62373; Q62374; Q62375 

Genbank Protein ID

 Y00054; M11942; BC061547; BC098914 

Genbank Nucleotide ID

 CAA68265.1; AAA41354.1; AAH61547.1; AAH98914.1 

Protein Name

 Heat shock cognate 71 kDa protein 

Protein Synonyms/Alias

 Heat shock 70 kDa protein 8 

Gene Name

 Hspa8 

Gene Synonyms/Alias

 Hsc70; Hsc73 

Created Date

 July 27, 2013 

Organism

 Rattus norvegicus (Rat) 

NCBI Taxa ID

 10116 

Lysine Modification

Position	Peptide	Type	References
56	RLIGDAAKNQVAMNP	ubiquitination	[1]
71	TNTVFDAKRLIGRRF	ubiquitination	[1]
108	PKVQVEYKGETKSFY	ubiquitination	[1]
159	DSQRQATKDAGTIAG	ubiquitination	[1]
187	AIAYGLDKKVGAERN	ubiquitination	[1]
319	GTLDPVEKALRDAKL	ubiquitination	[1]
325	EKALRDAKLDKSQIH	ubiquitination	[1]
451	EGERAMTKDNNLLGK	ubiquitination	[1]
500	KSTGKENKITITNDK	ubiquitination	[1]
507	KITITNDKGRLSKED	ubiquitination	[1]
512	NDKGRLSKEDIERMV	ubiquitination	[1]
524	RMVQEAEKYKAEDEK	ubiquitination	[1]
539	QRDKVSSKNSLESYA	ubiquitination	[1]
597	EEFEHQQKELEKVCN	ubiquitination	[1]

Reference

 [1] Synaptic protein ubiquitination in rat brain revealed by antibody-based ubiquitome analysis.
 Na CH, Jones DR, Yang Y, Wang X, Xu Y, Peng J.
 J Proteome Res. 2012 Sep 7;11(9):4722-32. [PMID: 22871113] 

Functional Description

 Acts as a repressor of transcriptional activation. Inhibits the transcriptional coactivator activity of CITED1 on Smad-mediated transcription. Chaperone. Component of the PRP19- CDC5L complex that forms an integral part of the spliceosome and is required for activating pre-mRNA splicing. May have a scaffolding role in the spliceosome assembly as it contacts all other components of the core complex (By similarity). 

Sequence Annotation

 REGION 186 377 Interaction with BAG1 (By similarity).
 MOD_RES 2 2 N-acetylserine (By similarity).
 MOD_RES 15 15 Phosphotyrosine (By similarity).
 MOD_RES 41 41 Phosphotyrosine (By similarity).
 MOD_RES 153 153 Phosphoserine (By similarity).
 MOD_RES 246 246 N6-acetyllysine (By similarity).
 MOD_RES 319 319 N6-acetyllysine (By similarity).
 MOD_RES 362 362 Phosphoserine (By similarity).
 MOD_RES 561 561 N6,N6,N6-trimethyllysine; by METTL21A; in
 MOD_RES 589 589 N6-acetyllysine (By similarity).
 MOD_RES 597 597 N6-acetyllysine (By similarity).
 MOD_RES 601 601 N6-acetyllysine (By similarity).  

Keyword

 3D-structure; Acetylation; ATP-binding; Chaperone; Complete proteome; Cytoplasm; Direct protein sequencing; Methylation; mRNA processing; mRNA splicing; Nucleotide-binding; Nucleus; Phosphoprotein; Reference proteome; Repressor; Spliceosome; Stress response; Transcription; Transcription regulation; Ubl conjugation. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 646 AA 

Protein Sequence

Gene Ontology

 GO:0070062; C:extracellular vesicular exosome; IDA:RGD.
 GO:0042470; C:melanosome; IEA:UniProtKB-SubCell.
 GO:0043005; C:neuron projection; IDA:RGD.
 GO:0043025; C:neuronal cell body; IDA:RGD.
 GO:0005730; C:nucleolus; ISS:UniProtKB.
 GO:0000974; C:Prp19 complex; ISS:UniProtKB.
 GO:0030529; C:ribonucleoprotein complex; ISS:UniProtKB.
 GO:0005681; C:spliceosomal complex; IEA:UniProtKB-KW.
 GO:0008021; C:synaptic vesicle; IDA:RGD.
 GO:0043531; F:ADP binding; IDA:RGD.
 GO:0005524; F:ATP binding; IDA:RGD.
 GO:0042623; F:ATPase activity, coupled; IDA:RGD.
 GO:0051082; F:unfolded protein binding; IMP:RGD.
 GO:0051085; P:chaperone mediated protein folding requiring cofactor; IDA:RGD.
 GO:0006397; P:mRNA processing; IEA:UniProtKB-KW.
 GO:0045892; P:negative regulation of transcription, DNA-dependent; ISS:UniProtKB.
 GO:0006950; P:response to stress; IEA:UniProtKB-KW.
 GO:0008380; P:RNA splicing; IEA:UniProtKB-KW.
 GO:0006351; P:transcription, DNA-dependent; IEA:UniProtKB-KW. 

Interpro

 IPR018181; Heat_shock_70_CS.
 IPR013126; Hsp_70_fam. 

Pfam

 PF00012; HSP70 

SMART

  

PROSITE

 PS00297; HSP70_1
 PS00329; HSP70_2
 PS01036; HSP70_3 

PRINTS

 PR00301; HEATSHOCK70.