CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-011144
UniProt Accession
Genbank Protein ID
 M62324 
Genbank Nucleotide ID
Protein Name
 AT-rich interactive domain-containing protein 5A 
Protein Synonyms/Alias
 ARID domain-containing protein 5A; Modulator recognition factor 1; MRF-1 
Gene Name
 ARID5A 
Gene Synonyms/Alias
 MRF1 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
85RVPHLGFKQINLWKIubiquitination[1, 2, 3, 4]
91FKQINLWKIYKAVEKubiquitination[3]
269KLLAQVSKVEALQCQubiquitination[3]
457EGAAHSGKRLRAVSPubiquitination[3]
Reference
 [1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [2] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
 [3] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [4] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789] 
Functional Description
 Binds to AT-rich stretches in the modulator region upstream of the human cytomegalovirus major intermediate early gene enhancer. May act as repressor and down-regulate enhancer- dependent gene expressison. 
Sequence Annotation
 DOMAIN 55 147 ARID.
 MOD_RES 23 23 Phosphoserine (By similarity).  
Keyword
 Complete proteome; DNA-binding; Nucleus; Phosphoprotein; Reference proteome; Repressor; Transcription; Transcription regulation. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 594 AA 
Protein Sequence
MAAPVKGNRK QSTEGDALDP PASPKPAGKQ NGIQNPISLE DSPEAGGERE EEQEREEEQA 60
FLVSLYKFMK ERHTPIERVP HLGFKQINLW KIYKAVEKLG AYELVTGRRL WKNVYDELGG 120
SPGSTSAATC TRRHYERLVL PYVRHLKGED DKPLPTSKPR KQYKMAKENR GDDGATERPK 180
KAKEERRMDQ MMPGKTKADA ADPAPLPSQE PPRNSTEQQG LASGSSVSFV GASGCPEAYK 240
RLLSSFYCKG THGIMSPLAK KKLLAQVSKV EALQCQEEGC RHGAEPQASP AVHLPESPQS 300
PKGLTENSRH RLTPQEGLQA PGGSLREEAQ AGPCPAAPIF KGCFYTHPTE VLKPVSQHPR 360
DFFSRLKDGV LLGPPGKEGL SVKEPQLVWG GDANRPSAFH KGGSRKGILY PKPKACWVSP 420
MAKVPAESPT LPPTFPSSPG LGSKRSLEEE GAAHSGKRLR AVSPFLKEAD AKKCGAKPAG 480
SGLVSCLLGP ALGPVPPEAY RGTMLHCPLN FTGTPGPLKG QAALPFSPLV IPAFPAHFLA 540
TAGPSPMAAG LMHFPPTSFD SALRHRLCPA SSAWHAPPVT TYAAPHFFHL NTKL 594 
Gene Ontology
 GO:0005730; C:nucleolus; IDA:HPA.
 GO:0005667; C:transcription factor complex; IEA:Compara.
 GO:0003682; F:chromatin binding; IEA:Compara.
 GO:0003677; F:DNA binding; TAS:GDB.
 GO:0002062; P:chondrocyte differentiation; IEA:Compara.
 GO:0045892; P:negative regulation of transcription, DNA-dependent; TAS:GDB.
 GO:0035066; P:positive regulation of histone acetylation; IEA:Compara.
 GO:0045944; P:positive regulation of transcription from RNA polymerase II promoter; IEA:Compara.
 GO:0006351; P:transcription, DNA-dependent; IEA:UniProtKB-KW. 
Interpro
 IPR001606; ARID/BRIGHT_DNA-bd. 
Pfam
 PF01388; ARID 
SMART
 SM00501; BRIGHT 
PROSITE
 PS51011; ARID 
PRINTS