UniProt Accession

 NS1_I72A2; P03495; Q1K9D5 

Genbank Protein ID

 V01102; DQ508933 

Genbank Nucleotide ID

 CAA24288.1; ABF21224.1 

Protein Name

 Non-structural protein 1 

Protein Synonyms/Alias

 NS1; NS1A 

Gene Name

 NS 

Gene Synonyms/Alias

  

Created Date

 July 27, 2013 

Organism

 Influenza A virus (strain A/Udorn/307/1972 H3N2) 

NCBI Taxa ID

 381517 

Lysine Modification

Position	Peptide	Type	References
219	PPLTPKQKRKMARTA	sumoylation	[1]
221	LTPKQKRKMARTARS	sumoylation	[1]

Reference

 [1] Modification of nonstructural protein 1 of influenza A virus by SUMO1.
 Xu K, Klenk C, Liu B, Keiner B, Cheng J, Zheng BJ, Li L, Han Q, Wang C, Li T, Chen Z, Shu Y, Liu J, Klenk HD, Sun B.
 J Virol. 2011 Jan;85(2):1086-98. [PMID: 21047957] 

Functional Description

 Inhibits post-transcriptional processing of cellular pre-mRNA, by binding and inhibiting two cellular proteins that are required for the 3'-end processing of cellular pre-mRNAs: the 30 kDa cleavage and polyadenylation specificity factor (CPSF4) and the poly(A)-binding protein 2 (PABPN1). This results in the accumulation of unprocessed 3' end pre-mRNAs which can't be exported from the nucleus. Cellular protein synthesis is thereby shut off very early after virus infection. Viral protein synthesis is not affected by the inhibition of the cellular 3' end processing machinery because the poly(A) tails of viral mRNAs are produced by the viral polymerase through a stuttering mechanism. 

Sequence Annotation

 REGION 1 73 RNA-binding and homodimerization.
 REGION 180 215 CPSF4-binding.
 REGION 223 237 PABPN1-binding.
 MOTIF 34 38 Nuclear localization signal 1.
 MOTIF 137 146 Nuclear export signal.
 MOTIF 216 221 Nuclear localization signal 2.  

Keyword

 3D-structure; Alternative splicing; Complete proteome; Host cytoplasm; Host gene expression shutoff by virus; Host mRNA suppression by virus; Host nucleus; Host-virus interaction; Inhibition of host IFN-mediated response initiation by virus; Inhibition of host innate immune response by virus; Inhibition of host interferon signaling pathway by virus; Inhibition of host PKR by virus; Inhibition of host pre-mRNA processing by virus; Inhibition of host RIG-I by virus; Interferon antiviral system evasion; RNA-binding; Suppressor of RNA silencing; Viral immunoevasion. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 237 AA 

Protein Sequence

Gene Ontology

 GO:0030430; C:host cell cytoplasm; IEA:UniProtKB-SubCell.
 GO:0042025; C:host cell nucleus; IEA:UniProtKB-SubCell.
 GO:0003725; F:double-stranded RNA binding; TAS:BHF-UCL.
 GO:0052038; P:modulation by symbiont of host intracellular transport; IDA:BHF-UCL.
 GO:0019056; P:modulation by virus of host transcription; IDA:BHF-UCL.
 GO:0052170; P:negative regulation by symbiont of host innate immune response; IDA:BHF-UCL.
 GO:0046775; P:suppression by virus of host cytokine production; IDA:BHF-UCL.
 GO:0039524; P:suppression by virus of host mRNA processing; IEA:UniProtKB-KW.
 GO:0039580; P:suppression by virus of host PKR activity; IEA:UniProtKB-KW.
 GO:0039540; P:suppression by virus of host RIG-I activity; IEA:UniProtKB-KW.
 GO:0039501; P:suppression by virus of host type I interferon production; IEA:UniProtKB-KW.
 GO:0039502; P:suppression by virus of host type I interferon-mediated signaling pathway; IEA:UniProtKB-KW. 

Interpro

 IPR000256; Flu_NS1.
 IPR009068; S15_NS1_RNA-bd. 

Pfam

 PF00600; Flu_NS1 

SMART

  

PROSITE

  

PRINTS