CPLM-000788

Genbank Nucleotide ID

Isocitrate dehydrogenase [NAD] subunit beta, mitochondrial

Protein Synonyms/Alias

Isocitric dehydrogenase subunit beta; NAD(+)-specific ICDH subunit beta

Homo sapiens (Human)

Position	Peptide	Type	References
96	QNMASEEKLEQVLSS	acetylation	[1, 2]
105	EQVLSSMKENKVAII	ubiquitination	[3]
135	YDMRLRRKLDLFANV	ubiquitination	[4, 5]
146	FANVVHVKSLPGYMT	acetylation	[1, 2]
199	AKSQRIAKFAFDYAT	acetylation	[1, 5]
199	AKSQRIAKFAFDYAT	ubiquitination	[4, 6, 7]
207	FAFDYATKKGRGKVT	acetylation	[2]
207	FAFDYATKKGRGKVT	ubiquitination	[6]
374	STTTDFIKSVIGHLQ	acetylation	[1]

[1] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861]
[2] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
PLoS One. 2012;7(12):e50545. [PMID: 23236377]
[3] Systematic and quantitative assessment of the ubiquitin-modified proteome.
Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
[4] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
[5] Integrated proteomic analysis of post-translational modifications by serial enrichment.
Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
[6] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
[7] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
Chen Z, Zhou Y, Song J, Zhang Z.
Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]

Functional Description

METAL 251 251 Magnesium or manganese (By similarity).
BINDING 133 133 Substrate (By similarity).
BINDING 164 164 Substrate (By similarity).
BINDING 251 251 Substrate (By similarity).
MOD_RES 199 199 N6-acetyllysine.

Acetylation; Alternative splicing; Complete proteome; Disease mutation; Magnesium; Manganese; Metal-binding; Mitochondrion; NAD; Oxidoreductase; Polymorphism; Reference proteome; Retinitis pigmentosa; Transit peptide; Tricarboxylic acid cycle.

UniProt (SWISSPROT/TrEMBL); GenBank; EMBL

GO:0005759; C:mitochondrial matrix; TAS:Reactome.
GO:0009055; F:electron carrier activity; TAS:UniProtKB.
GO:0004449; F:isocitrate dehydrogenase (NAD+) activity; TAS:ProtInc.
GO:0000287; F:magnesium ion binding; IEA:InterPro.
GO:0051287; F:NAD binding; IEA:InterPro.
GO:0006103; P:2-oxoglutarate metabolic process; IEA:Compara.
GO:0006102; P:isocitrate metabolic process; TAS:ProtInc.
GO:0006734; P:NADH metabolic process; IEA:Compara.
GO:0006099; P:tricarboxylic acid cycle; TAS:Reactome.

IPR019818; IsoCit/isopropylmalate_DH_CS.
IPR001804; Isocitrate/isopropylmalate_DH.
IPR004434; Isocitrate_DH_NAD.
IPR024084; IsoPropMal-DH-like_dom.