CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-008967
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Caspase-9 
Protein Synonyms/Alias
 CASP-9; Apoptotic protease Mch-6; Apoptotic protease-activating factor 3; APAF-3; ICE-like apoptotic protease 6; ICE-LAP6; Caspase-9 subunit p35; Caspase-9 subunit p10 
Gene Name
 CASP9 
Gene Synonyms/Alias
 MCH6 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
189GSNIDCEKLRRRFSSubiquitination[1]
394VANAVSVKGIYKQMPubiquitination[1]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961] 
Functional Description
 Involved in the activation cascade of caspases responsible for apoptosis execution. Binding of caspase-9 to Apaf- 1 leads to activation of the protease which then cleaves and activates caspase-3. Promotes DNA damage-induced apoptosis in a ABL1/c-Abl-dependent manner. Proteolytically cleaves poly(ADP- ribose) polymerase (PARP). 
Sequence Annotation
 DOMAIN 1 92 CARD.
 ACT_SITE 237 237 By similarity.
 ACT_SITE 287 287 By similarity.
 MOD_RES 125 125 Phosphothreonine; by MAPK1.
 MOD_RES 153 153 Phosphotyrosine; by ABL1.
 MOD_RES 302 302 Phosphoserine.
 MOD_RES 307 307 Phosphoserine.  
Keyword
 3D-structure; Alternative splicing; Apoptosis; Complete proteome; Hydrolase; Phosphoprotein; Polymorphism; Protease; Reference proteome; Thiol protease; Zymogen. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 416 AA 
Protein Sequence
MDEADRRLLR RCRLRLVEEL QVDQLWDALL SRELFRPHMI EDIQRAGSGS RRDQARQLII 60
DLETRGSQAL PLFISCLEDT GQDMLASFLR TNRQAAKLSK PTLENLTPVV LRPEIRKPEV 120
LRPETPRPVD IGSGGFGDVG ALESLRGNAD LAYILSMEPC GHCLIINNVN FCRESGLRTR 180
TGSNIDCEKL RRRFSSLHFM VEVKGDLTAK KMVLALLELA QQDHGALDCC VVVILSHGCQ 240
ASHLQFPGAV YGTDGCPVSV EKIVNIFNGT SCPSLGGKPK LFFIQACGGE QKDHGFEVAS 300
TSPEDESPGS NPEPDATPFQ EGLRTFDQLD AISSLPTPSD IFVSYSTFPG FVSWRDPKSG 360
SWYVETLDDI FEQWAHSEDL QSLLLRVANA VSVKGIYKQM PGCFNFLRKK LFFKTS 416 
Gene Ontology
 GO:0043293; C:apoptosome; IDA:UniProtKB.
 GO:0005634; C:nucleus; IEA:Compara.
 GO:0004197; F:cysteine-type endopeptidase activity; TAS:ProtInc.
 GO:0008047; F:enzyme activator activity; TAS:ProtInc.
 GO:0019901; F:protein kinase binding; IDA:UniProtKB.
 GO:0017124; F:SH3 domain binding; IDA:UniProtKB.
 GO:0008635; P:activation of cysteine-type endopeptidase activity involved in apoptotic process by cytochrome c; TAS:Reactome.
 GO:0071549; P:cellular response to dexamethasone stimulus; IEA:Compara.
 GO:0034644; P:cellular response to UV; IDA:UniProtKB.
 GO:0007173; P:epidermal growth factor receptor signaling pathway; TAS:Reactome.
 GO:0008543; P:fibroblast growth factor receptor signaling pathway; TAS:Reactome.
 GO:0045087; P:innate immune response; TAS:Reactome.
 GO:0008630; P:intrinsic apoptotic signaling pathway in response to DNA damage; IMP:UniProtKB.
 GO:0048011; P:neurotrophin TRK receptor signaling pathway; TAS:Reactome.
 GO:0048015; P:phosphatidylinositol-mediated signaling; TAS:Reactome.
 GO:0043525; P:positive regulation of neuron apoptotic process; IEA:Compara.
 GO:0006508; P:proteolysis; IEA:UniProtKB-KW.
 GO:2001020; P:regulation of response to DNA damage stimulus; IMP:UniProtKB.
 GO:0042770; P:signal transduction in response to DNA damage; IDA:UniProtKB. 
Interpro
 IPR001315; CARD.
 IPR017350; Caspase_IL-1_beta.
 IPR011029; DEATH-like_dom.
 IPR011600; Pept_C14_cat.
 IPR001309; Pept_C14_ICE_p20.
 IPR016129; Pept_C14_ICE_p20_AS.
 IPR002138; Pept_C14_p10.
 IPR002398; Pept_C14_p45.
 IPR015917; Pept_C14_p45_core. 
Pfam
 PF00619; CARD
 PF00656; Peptidase_C14 
SMART
 SM00114; CARD
 SM00115; CASc 
PROSITE
 PS50209; CARD
 PS01122; CASPASE_CYS
 PS01121; CASPASE_HIS
 PS50207; CASPASE_P10
 PS50208; CASPASE_P20 
PRINTS
 PR00376; IL1BCENZYME.