CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-021295
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Gigaxonin 
Protein Synonyms/Alias
 Kelch-like protein 16 
Gene Name
 GAN 
Gene Synonyms/Alias
 GAN1; KLHL16 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
177FLELSPQKLKEVISLubiquitination[1]
246PLVREIVKECSNIPLubiquitination[2]
346QTLSSGEKYDPDANTubiquitination[1]
500EFYHDEFKRWIYLNDubiquitination[2]
579ALRIANCKLFRLQLQubiquitination[2]
Reference
 [1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961] 
Functional Description
 Probable cytoskeletal component that directly or indirectly plays an important role in neurofilament architecture. May act as a substrate-specific adapter of an E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Controls degradation of TBCB. Controls degradation of MAP1B and MAP1S, and is critical for neuronal maintenance and survival. 
Sequence Annotation
 DOMAIN 30 99 BTB.
 DOMAIN 134 236 BACK.
 REPEAT 274 326 Kelch 1.
 REPEAT 327 374 Kelch 2.
 REPEAT 376 421 Kelch 3.
 REPEAT 422 468 Kelch 4.
 REPEAT 470 522 Kelch 5.
 REPEAT 528 574 Kelch 6.
 MOD_RES 8 8 Phosphoserine (By similarity).
 MOD_RES 471 471 Phosphotyrosine (By similarity).  
Keyword
 3D-structure; Complete proteome; Cytoplasm; Cytoskeleton; Disease mutation; Kelch repeat; Neurodegeneration; Phosphoprotein; Polymorphism; Reference proteome; Repeat; Ubl conjugation; Ubl conjugation pathway. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 597 AA 
Protein Sequence
MAEGSAVSDP QHAARLLRAL SSFREESRFC DAHLVLDGEE IPVQKNILAA ASPYIRTKLN 60
YNPPKDDGST YKIELEGISV MVMREILDYI FSGQIRLNED TIQDVVQAAD LLLLTDLKTL 120
CCEFLEGCIA AENCIGIRDF ALHYCLHHVH YLATEYLETH FRDVSSTEEF LELSPQKLKE 180
VISLEKLNVG NERYVFEAVI RWIAHDTEIR KVHMKDVMSA LWVSGLDSSY LREQMLNEPL 240
VREIVKECSN IPLSQPQQGE AMLANFKPRG YSECIVTVGG EERVSRKPTA AMRCMCPLYD 300
PNRQLWIELA PLSMPRINHG VLSAEGFLFV FGGQDENKQT LSSGEKYDPD ANTWTALPPM 360
NEARHNFGIV EIDGMLYILG GEDGEKELIS MECYDIYSKT WTKQPDLTMV RKIGCYAAMK 420
KKIYAMGGGS YGKLFESVEC YDPRTQQWTA ICPLKERRFG AVACGVAMEL YVFGGVRSRE 480
DAQGSEMVTC KSEFYHDEFK RWIYLNDQNL CIPASSSFVY GAVPIGASIY VIGDLDTGTN 540
YDYVREFKRS TGTWHHTKPL LPSDLRRTGC AALRIANCKL FRLQLQQGLF RIRVHSP 597 
Gene Ontology
 GO:0031463; C:Cul3-RING ubiquitin ligase complex; IDA:UniProtKB.
 GO:0005737; C:cytoplasm; IDA:UniProtKB.
 GO:0005883; C:neurofilament; NAS:UniProtKB.
 GO:0008219; P:cell death; IEA:UniProtKB-KW.
 GO:0016567; P:protein ubiquitination; IDA:UniProtKB. 
Interpro
 IPR011705; BACK.
 IPR000210; BTB/POZ-like.
 IPR011333; BTB/POZ_fold.
 IPR013069; BTB_POZ.
 IPR017096; Kelch-like_gigaxonin.
 IPR015915; Kelch-typ_b-propeller.
 IPR006652; Kelch_1. 
Pfam
 PF07707; BACK
 PF00651; BTB
 PF01344; Kelch_1 
SMART
 SM00875; BACK
 SM00225; BTB
 SM00612; Kelch 
PROSITE
 PS50097; BTB 
PRINTS