UniProt Accession

 NUDJ_ECOLI; P0AEI6; P75965 

Genbank Protein ID

 U00096; AP009048 

Genbank Nucleotide ID

 AAC74218.1; BAA35956.1 

Protein Name

 Phosphatase NudJ 

Protein Synonyms/Alias

  

Gene Name

 nudJ 

Gene Synonyms/Alias

 ymfB; b1134; JW1120 

Created Date

 July 27, 2013 

Organism

 Escherichia coli (strain K12) 

NCBI Taxa ID

 83333 

Lysine Modification

Position	Peptide	Type	References
76	HQWIAPDKTPFLRFL	acetylation	[1]

Reference

 [1] Acetyl-Phosphate Is a Critical Determinant of Lysine Acetylation in E. coli.
 Weinert BT, Iesmantavicius V, Wagner SA, Schölz C, Gummesson B, Beli P, Nyström T, Choudhary C.
 Mol Cell. 2013 Jul 25;51(2):265-72. [PMID: 23830618] 

Functional Description

 Catalyzes the hydrolysis of 4-amino-2-methyl-5- hydroxymethylpyrimidine pyrophosphate (HMP-PP) to 4-amino-2- methyl-5-hydroxymethylpyrimidine phosphate (HMP-P), and hydrolysis of thiamine pyrophosphate (TPP) to thiamine monophosphate (TMP). Can hydrolyze other substrates such as MeO-HMP-PP, CF(3)-HMP-PP and MeO-TPP. Is also a non-specific nucleoside tri- and diphosphatase that releases inorganic orthophosphate. 

Sequence Annotation

 DOMAIN 3 131 Nudix hydrolase.
 MOTIF 36 57 Nudix box.  

Keyword

 Complete proteome; Hydrolase; Magnesium; Reference proteome. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 153 AA 

Protein Sequence

Gene Ontology

 GO:0016787; F:hydrolase activity; IEA:UniProtKB-KW. 

Interpro

 IPR020476; Nudix_hydrolase.
 IPR020084; NUDIX_hydrolase_CS.
 IPR000086; NUDIX_hydrolase_dom.
 IPR015797; NUDIX_hydrolase_dom-like. 

Pfam

 PF00293; NUDIX 

SMART

  

PROSITE

 PS51462; NUDIX
 PS00893; NUDIX_BOX 

PRINTS

 PR00502; NUDIXFAMILY.